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- SASDFQ4: Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFQ4
SampleDomain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C)
  • Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) (protein), ProMMP-1 (S243C, S31, Homo sapiens
Function / homology
Function and homology information


interstitial collagenase / cellular response to UV-A / Basigin interactions / Activation of Matrix Metalloproteinases / Collagen degradation / collagen catabolic process / extracellular matrix disassembly / Degradation of the extracellular matrix / extracellular matrix organization / extracellular matrix ...interstitial collagenase / cellular response to UV-A / Basigin interactions / Activation of Matrix Metalloproteinases / Collagen degradation / collagen catabolic process / extracellular matrix disassembly / Degradation of the extracellular matrix / extracellular matrix organization / extracellular matrix / positive regulation of protein-containing complex assembly / metalloendopeptidase activity / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / peptidase activity / Interleukin-4 and Interleukin-13 signaling / endopeptidase activity / serine-type endopeptidase activity / proteolysis / extracellular region / zinc ion binding
Similarity search - Function
Hemopexin, conserved site / Hemopexin domain signature. / Hemopexin-like domain / Peptidoglycan binding-like / Peptidase M10A, cysteine switch, zinc binding site / Matrixins cysteine switch. / Hemopexin-like repeats / Hemopexin-like domain superfamily / Hemopexin / Putative peptidoglycan binding domain ...Hemopexin, conserved site / Hemopexin domain signature. / Hemopexin-like domain / Peptidoglycan binding-like / Peptidase M10A, cysteine switch, zinc binding site / Matrixins cysteine switch. / Hemopexin-like repeats / Hemopexin-like domain superfamily / Hemopexin / Putative peptidoglycan binding domain / Hemopexin repeat profile. / Hemopexin-like repeats. / Peptidase M10A / Peptidase M10A, catalytic domain / Peptidase M10, metallopeptidase / Matrixin / PGBD-like superfamily / Peptidase, metallopeptidase / Zinc-dependent metalloprotease / Metallopeptidase, catalytic domain superfamily / Neutral zinc metallopeptidases, zinc-binding region signature.
Similarity search - Domain/homology
Interstitial collagenase
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Rob Holland (University of Portsmouth)

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Structure visualization

Downloads & links

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Models

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Sample

SampleName: Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C)
Specimen concentration: 5.6 mg/ml
BufferName: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride
pH: 7.4
Entity #1573Name: ProMMP-1 (S243C, S31 / Type: protein
Description: Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C)
Formula weight: 52.007 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P03956
Sequence: MFPATLETQE QDVDLVQKYL EKYYNLKNDG RQVEKRRNSG PVVEKLKQMQ EFFGLKVTGK PDAETLKVMK QPRCGVPDVA QFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG ...Sequence:
MFPATLETQE QDVDLVQKYL EKYYNLKNDG RQVEKRRNSG PVVEKLKQMQ EFFGLKVTGK PDAETLKVMK QPRCGVPDVA QFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFCGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIC VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 3.9 mm
DetectorName: Pilatus 2M
Scan
Title: Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C)
Measurement date: May 21, 2015 / Storage temperature: 4 °C / Cell temperature: 25 °C / Exposure time: 3 sec. / Number of frames: 28 / Unit: 1/A /
MinMax
Q0.0033 0.4038
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 866 /
MinMax
Q0.0126242 0.250388
P(R) point1 866
R0 89
Result
Type of curve: sec /
ExperimentalPorod
MW39.35 kDa38 kDa
Volume-61 nm3

P(R)GuinierGuinier error
Forward scattering, I00.01028 0.01029 5.1E-5
Radius of gyration, Rg2.71 nm2.694 nm0.021

MinMax
D-8.9
Guinier point35 164

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