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- SASDFQ2: Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294) -
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Open data
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Basic information
Entry | ![]() |
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![]() | Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294)
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Function / homology | ![]() peptidoglycan-based cell wall biogenesis / regulation of growth rate / cell pole / peptidoglycan-based cell wall / regulation of cell shape / protein stabilization / cell division / plasma membrane / cytoplasm Similarity search - Function |
Biological species | ![]() |
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Structure visualization
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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-Models
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Sample
![]() | Name: Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294) Specimen concentration: 2.4 mg/ml |
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Buffer | Name: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME) pH: 7.5 |
Entity #1212 | Type: protein / Description: Cell wall synthesis protein Wag31 / Formula weight: 29.98 Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) References: UniProt: P9WMU1 Sequence: MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAEQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ...Sequence: MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAEQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ADAMLADAQS RSEAQLRQAQ EKADALQADA ERKHSEIMGT INQQRAVLEG RLEQLRTFER EYRTRLKTYL ESQLEELGQR GSAAPVDSNA DAGGFDQFNR GKN |
-Experimental information
Beam | Instrument name: Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2 City: Stanford, CA / 国: USA ![]() | ||||||
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Detector | Name: Rayonix MX225-HE | ||||||
Scan | Measurement date: Jan 29, 2018 / Storage temperature: 23 °C / Cell temperature: 23 °C / Exposure time: 1 sec. / Number of frames: 500 / Unit: 1/A /
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Result | Experimental MW: 29.97 kDa / I0 from Guinier: 11.11 / Rg from Guinier: 8.061 nm / Type of curve: sec Comments: SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: ...Comments: SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 290-294
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