SASDFP6: 80 base pair DNA (80bp_DNA Forward + 80bp_DNA Reverse)

Basic information

• Entry: Database: SASBDB / ID: SASDFP6

Sample

80 base pair DNA

• 80bp_DNA Forward (DNA), Escherichia coli
• 80bp_DNA Reverse (DNA), Escherichia coli K-12

• Biological species: Escherichia coli (E. coli); Escherichia coli K-12 (bacteria)

Contact author

• Soumya G Remesh (Lawrence Berkeley National laboratory)

Models

• Model #2975: ; Type: atomic / Chi-square value: 129.829249053513

Sample

• Sample: Name: 80 base pair DNA / Entity id: 1622 / 1623
• Buffer: Name: 10 mM Bis-Tris, 100 mM NaCl / pH: 7.5

Entity #1622

Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli
Sequence:

AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT

Entity #1623

Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12
Sequence:

TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA

Experimental information

• Beam: Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS); City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
• Detector: Name: Pilatus3 X 2M / Pixsize x: 172 mm

Scan

Title: 80 base pair DNA / Measurement date: Jun 1, 2018 / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 300 / Unit: 1/A /

	Min	Max
Q	0.01	0.3238

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 499 /

	Min	Max
Q	0.01064	0.3135
P(R) point	1	499
R	0	288.5

Result

Experimental MW: 59 kDa / Type of curve: single_conc

	P(R)	Guinier	Guinier error
Forward scattering, I0	814.9	779.47	5.13
Radius of gyration, Rg	7.74 nm	6.98 nm	0.211

	Min	Max
D	-	28.85
Guinier point	2	15