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- SASDFL2: Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274) -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFL2
SampleCell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)
  • Cell wall synthesis protein Wag31 (protein), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function / homology
Function and homology information


peptidoglycan-based cell wall biogenesis / regulation of growth rate / cell pole / peptidoglycan-based cell wall / regulation of cell shape / protein stabilization / cell division / plasma membrane / cytoplasm
Similarity search - Function
DivIVA family / DivIVA domain / DivIVA protein
Similarity search - Domain/homology
Cell wall synthesis protein Wag31
Similarity search - Component
Biological speciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Contact author
  • Komal Choukate (CSIR, CSIR-Institute of Microbial Technology, Chandigarh, India)

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Structure visualization

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Models

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Sample

SampleName: Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)
Specimen concentration: 2.4 mg/ml
BufferName: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)
pH: 7.5
Entity #1212Type: protein / Description: Cell wall synthesis protein Wag31 / Formula weight: 29.98
Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
References: UniProt: P9WMU1
Sequence: MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAEQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ...Sequence:
MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAEQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ADAMLADAQS RSEAQLRQAQ EKADALQADA ERKHSEIMGT INQQRAVLEG RLEQLRTFER EYRTRLKTYL ESQLEELGQR GSAAPVDSNA DAGGFDQFNR GKN

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Experimental information

BeamInstrument name: Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2
City: Stanford, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 2.54 mm
DetectorName: Rayonix MX225-HE
Scan
Title: Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)
Measurement date: Jan 29, 2018 / Storage temperature: 23 °C / Cell temperature: 23 °C / Exposure time: 1 sec. / Number of frames: 500 / Unit: 1/A /
MinMax
Q0.0055 0.4023
ResultExperimental MW: 29.97 kDa / I0 from Guinier: 20.04 / Rg from Guinier: 9.009 nm / Type of curve: sec
Comments: SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: ...Comments: SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 270-274
MinMax
Guinier point6 21

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