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Yorodumi- SASDFH6: Probable exodeoxyribonuclease III protein XthA, sliding beta clam... -
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-Basic information
Entry | Database: SASBDB / ID: SASDFH6 |
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Sample | Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate
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Biological species | Mycobacterium tuberculosis (bacteria) Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria) |
-Structure visualization
-Downloads & links
-Data source
SASBDB page | SASDFH6 |
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-Models
-Sample
Sample | Name: Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate Specimen concentration: 2.5 mg/ml / Entity id: 1670 / 1671 / 1672 / 1673 |
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Buffer | Name: 50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol pH: 8 |
Entity #1670 | Name: MtbXthA / Type: protein / Description: Probable exodeoxyribonuclease III protein XthA / Formula weight: 30.699 / Num. of mol.: 1 / Source: Mycobacterium tuberculosis Sequence: MRLATWNVNS VKARLPRLLA WLDETKPDVV CLQETKSAAD GFPSGDVAEL GYTTAAYGEG RWNGVAILSR VGIEDVSKSF PGEPVYQGEA PAGPGVQTQL TEPVLLAPQA RAIGATCGGI RVWSLYAPNG RTPESAHYLY KLEWFAALRD ALAAELSAGT PLVATGDYNV ...Sequence: MRLATWNVNS VKARLPRLLA WLDETKPDVV CLQETKSAAD GFPSGDVAEL GYTTAAYGEG RWNGVAILSR VGIEDVSKSF PGEPVYQGEA PAGPGVQTQL TEPVLLAPQA RAIGATCGGI RVWSLYAPNG RTPESAHYLY KLEWFAALRD ALAAELSAGT PLVATGDYNV APTDDDVWDP SVFVGSTHVT PAERQALAEL RDLGLHDIVP TPMKGPHPYT YWDYRAGSFH KDLGMRIDLV YGNGAFAERV RSAYVDREAR KGRGPSDHAP VVVDLDHHHH HH |
Entity #1671 | Type: protein / Description: DNA ligase A / Formula weight: 76.111 / Num. of mol.: 1 Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Sequence: MSSPDADQTA PEVLRQWQAL AEEVREHQFR YYVRDAPIIS DAEFDELLRR LEALEEQHPE LRTPDSPTQL VGGAGFATDF EPVDHLERML SLDNAFTADE LAAWAGRIHA EVGDAAHYLC ELKIDGVALS LVYREGRLTR ASTRGDGRTG EDVTLNARTI ADVPERLTPG ...Sequence: MSSPDADQTA PEVLRQWQAL AEEVREHQFR YYVRDAPIIS DAEFDELLRR LEALEEQHPE LRTPDSPTQL VGGAGFATDF EPVDHLERML SLDNAFTADE LAAWAGRIHA EVGDAAHYLC ELKIDGVALS LVYREGRLTR ASTRGDGRTG EDVTLNARTI ADVPERLTPG DDYPVPEVLE VRGEVFFRLD DFQALNASLV EEGKAPFANP RNSAAGSLRQ KDPAVTARRR LRMICHGLGH VEGFRPATLH QAYLALRAWG LPVSEHTTLA TDLAGVRERI DYWGEHRHEV DHEIDGVVVK VDEVALQRRL GSTSRAPRWA IAYKYPPEEA QTKLLDIRVN VGRTGRITPF AFMTPVKVAG STVGQATLHN ASEIKRKGVL IGDTVVIRKA GDVIPEVLGP VVELRDGSER EFIMPTTCPE CGSPLAPEKE GDADIRCPNA RGCPGQLRER VFHVASRNGL DIEVLGYEAG VALLQAKVIA DEGELFALTE RDLLRTDLFR TKAGELSANG KRLLVNLDKA KAAPLWRVLV ALSIRHVGPT AARALATEFG SLDAIAAAST DQLAAVEGVG PTIAAAVTEW FAVDWHREIV DKWRAAGVRM VDERDESVPR TLAGLTIVVT GSLTGFSRDD AKEAIVARGG KAAGSVSKKT NYVVAGDSPG SKYDKAVELG VPILDEDGFR RLLADGPASR THHHHHH |
Entity #1672 | Type: protein / Description: Beta sliding clamp / Formula weight: 42.935 / Num. of mol.: 2 Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Sequence: MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVDV HVEGNRVALT CGNARFSLPT MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ...Sequence: MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVDV HVEGNRVALT CGNARFSLPT MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ILGETVVLAA TDRFRLAVRE LKWSASSPDI EAAVLVPAKT LAEAAKAGIG GSDVRLSLGT GPGVGKDGLL GISGNGKRST TRLLDAEFPK FRQLLPTEHT AVATMDVAEL IEAIKLVALV ADRGAQVRME FADGSVRLSA GADDVGRAEE DLVVDYAGEP LTIAFNPTYL TDGLSSLRSE RVSFGFTTAG KPALLRPVSG DDRPVAGLNG NGPFPAVSTD YVYLLMPVRL PGHHHHHH |
Entity #1673 | Type: DNA / Description: DNA ligase A nicked DNA substrate / Formula weight: 8.1 / Num. of mol.: 2 Sequence: GGTAGATCAG TGTCTATGTA TGTCAG |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.0992 Å / Dist. spec. to detc.: 2.9 mm | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
Scan | Title: Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate Measurement date: May 17, 2017 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
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Result | Type of curve: single_conc Comments: The presented model shows the spatial alignment of several individually calculated models and does not fit the SAXS data. A corresponding individual model fit (individual model not provided ...Comments: The presented model shows the spatial alignment of several individually calculated models and does not fit the SAXS data. A corresponding individual model fit (individual model not provided for this entry) is shown. The sample is severely aggregated.
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