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- SASDFF3: Filamin A Ig-like domains 4-6, V711D mutant (FLNa4-6 V711D) at 2 mg/ml -

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Basic information

Entry
Database: SASBDB / ID: SASDFF3
SampleFilamin A Ig-like domains 4-6, V711D mutant (FLNa4-6 V711D) at 2 mg/ml
  • Filamin A Ig-like domains 4-6, V711D mutation (protein), FLNa4-6 V711D, Homo sapiens
Function / homology
Function and homology information


regulation of membrane repolarization during atrial cardiac muscle cell action potential / regulation of membrane repolarization during cardiac muscle cell action potential / establishment of Sertoli cell barrier / Myb complex / glycoprotein Ib-IX-V complex / adenylate cyclase-inhibiting dopamine receptor signaling pathway / formation of radial glial scaffolds / positive regulation of integrin-mediated signaling pathway / : / tubulin deacetylation ...regulation of membrane repolarization during atrial cardiac muscle cell action potential / regulation of membrane repolarization during cardiac muscle cell action potential / establishment of Sertoli cell barrier / Myb complex / glycoprotein Ib-IX-V complex / adenylate cyclase-inhibiting dopamine receptor signaling pathway / formation of radial glial scaffolds / positive regulation of integrin-mediated signaling pathway / : / tubulin deacetylation / actin crosslink formation / blood coagulation, intrinsic pathway / protein localization to bicellular tight junction / OAS antiviral response / positive regulation of actin filament bundle assembly / positive regulation of neuron migration / positive regulation of potassium ion transmembrane transport / Cell-extracellular matrix interactions / early endosome to late endosome transport / Fc-gamma receptor I complex binding / apical dendrite / cell-cell junction organization / positive regulation of neural precursor cell proliferation / positive regulation of platelet activation / protein localization to cell surface / wound healing, spreading of cells / podosome / negative regulation of transcription by RNA polymerase I / megakaryocyte development / GP1b-IX-V activation signalling / positive regulation of axon regeneration / cortical cytoskeleton / receptor clustering / SMAD binding / RHO GTPases activate PAKs / actin filament bundle / brush border / semaphorin-plexin signaling pathway / blood vessel remodeling / cilium assembly / mitotic spindle assembly / epithelial to mesenchymal transition / potassium channel regulator activity / heart morphogenesis / axonal growth cone / release of sequestered calcium ion into cytosol / positive regulation of substrate adhesion-dependent cell spreading / regulation of cell migration / dendritic shaft / protein kinase C binding / protein localization to plasma membrane / actin filament / negative regulation of DNA-binding transcription factor activity / trans-Golgi network / mRNA transcription by RNA polymerase II / G protein-coupled receptor binding / synapse organization / establishment of protein localization / negative regulation of protein catabolic process / cerebral cortex development / small GTPase binding / kinase binding / platelet aggregation / Z disc / positive regulation of protein import into nucleus / actin filament binding / actin cytoskeleton / cell-cell junction / Platelet degranulation / negative regulation of neuron projection development / GTPase binding / actin cytoskeleton organization / angiogenesis / DNA-binding transcription factor binding / positive regulation of canonical NF-kappaB signal transduction / perikaryon / transmembrane transporter binding / postsynapse / protein stabilization / cadherin binding / focal adhesion / negative regulation of apoptotic process / nucleolus / perinuclear region of cytoplasm / glutamatergic synapse / protein homodimerization activity / RNA binding / extracellular exosome / extracellular region / nucleus / membrane / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Filamin family / Filamin/ABP280 repeat / Filamin-type immunoglobulin domains / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. / Actinin-type actin-binding domain, conserved site / Calponin homology domain ...Filamin family / Filamin/ABP280 repeat / Filamin-type immunoglobulin domains / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. / Actinin-type actin-binding domain, conserved site / Calponin homology domain / Calponin homology (CH) domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Immunoglobulin E-set / Immunoglobulin-like fold
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
CitationDate: 2019 Aug
Title: Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia
Authors: Haataja T / Capoulade R / Lecointe S / Hellman M / Merot J / Permi P
Contact author
  • Tatu Haataja (University of Jyväskylä, Jyväskylän, Finland)

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Structure visualization

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Models

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Sample

SampleName: Filamin A Ig-like domains 4-6, V711D mutant (FLNa4-6 V711D) at 2 mg/ml
Specimen concentration: 2 mg/ml
BufferName: 20 mM Tris, 100 mM NaCl, 1 mM DTT / pH: 8
Entity #1500Name: FLNa4-6 V711D / Type: protein / Description: Filamin A Ig-like domains 4-6, V711D mutation / Formula weight: 31.536 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P21333
Sequence: MSCGNQKVRA WGPGLEGGVV GKSADFVVEA IGDDVGTLGF SVEGPSQAKI ECDDKGDGSC DVRYWPQEAG EYAVHVLCNS EDIRLSPFMA DIRDAPQDFH PDRVKARGPG LEKTGVAVNK PAEFTVDAKH GGKAPLRVQD QDNEGCPVEA LVKDNGNGTY SCSYVPRKPV ...Sequence:
MSCGNQKVRA WGPGLEGGVV GKSADFVVEA IGDDVGTLGF SVEGPSQAKI ECDDKGDGSC DVRYWPQEAG EYAVHVLCNS EDIRLSPFMA DIRDAPQDFH PDRVKARGPG LEKTGVAVNK PAEFTVDAKH GGKAPLRVQD QDNEGCPVEA LVKDNGNGTY SCSYVPRKPV KHTAMVSWGG VSIPNSPFRV NVGAGSHPNK VKVYGPGVAK TGLKAHEPTY FTVDCAEAGQ GDVSIGIKCA PGVVGPAEAD IDFDIIRNDN DTFTVKYTPR GAGSYTIMVL FADQATPTSP IRVKVEPS

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 2.9 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Filamin A Ig-like domains 4-6, V711D mutant (FLNa4-6 V711D) at 2 mg/ml
Measurement date: Feb 10, 2017 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0365 4.9417
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 291 /
MinMax
Q0.1283 2.856
P(R) point20 310
R0 14.8
Result
Type of curve: single_conc
Comments: Three-domain fragment of human Filamin A Ig-like domains 4-6 with V711D mutation (FLNa4-6 V711D) . There are two extra amino acids, Met and Ser, in the very N-terminus of the polypeptide ...Comments: Three-domain fragment of human Filamin A Ig-like domains 4-6 with V711D mutation (FLNa4-6 V711D) . There are two extra amino acids, Met and Ser, in the very N-terminus of the polypeptide that have resulted from TEV-cleavage of the GST fusion tag during the protein purification.
ExperimentalPorod
MW26.2 kDa35 kDa
Volume-57 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I026.74 0.05 26.86 0.05
Radius of gyration, Rg4.03 nm0.04 3.95 nm0.14

MinMax
D-14.8
Guinier point5 49

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