SASDFE6: Sliding beta clamp (Beta sliding clamp)

Basic information

• Entry: Database: SASBDB / ID: SASDFE6

Sample

Sliding beta clamp

• Beta sliding clamp (protein), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Function / homology

Function:

DNA polymerase III complex / DNA strand elongation involved in DNA replication / 3'-5' exonuclease activity / peptidoglycan-based cell wall / DNA-directed DNA polymerase activity / response to antibiotic / DNA binding / extracellular region / identical protein binding / cytoplasm

Domain/homology:

DNA polymerase III, beta sliding clamp / DNA polymerase III, beta sliding clamp, N-terminal / DNA polymerase III, beta sliding clamp, C-terminal / DNA polymerase III, beta sliding clamp, central / DNA polymerase III beta subunit, N-terminal domain / DNA polymerase III beta subunit, central domain / DNA polymerase III beta subunit, C-terminal domain / DNA polymerase III beta subunit / :

Component:

Beta sliding clamp

• Biological species: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)

Contact author

• Ravishankar Ramachandran (CSIR-Central Drug Research Institute)

Models

• Model #3014: ; Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 1.06

Sample

• Sample: Name: Sliding beta clamp / Specimen concentration: 2 mg/ml
• Buffer: Name: 50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol; pH: 8

Entity #1652

Type: protein / Description: Beta sliding clamp / Formula weight: 42.935 / Num. of mol.: 2
Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
References: UniProt: P9WNU1
Sequence:

MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVDV HVEGNRVALT CGNARFSLPT MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ILGETVVLAA TDRFRLAVRE LKWSASSPDI EAAVLVPAKT LAEAAKAGIG GSDVRLSLGT GPGVGKDGLL GISGNGKRST TRLLDAEFPK FRQLLPTEHT AVATMDVAEL IEAIKLVALV ADRGAQVRME FADGSVRLSA GADDVGRAEE DLVVDYAGEP LTIAFNPTYL TDGLSSLRSE RVSFGFTTAG KPALLRPVSG DDRPVAGLNG NGPFPAVSTD YVYLLMPVRL PGHHHHHH

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.0992 Å / Dist. spec. to detc.: 2.867 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Sliding beta clamp / Measurement date: May 15, 2017 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /

	Min	Max
Q	0.0308	4.9444

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 508 /

	Min	Max
Q	0.187343	2.59198
P(R) point	1	508
R	0	9.16

Result

Type of curve: single_conc / Comments: The sample shows signs of severe aggregation. /

	Experimental	Porod
MW	90 kDa	99 kDa
Volume	-	158 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	9.325	9.37	0.32
Radius of gyration, Rg	3.611 nm	3.71 nm	0.14

	Min	Max
D	-	9.16
Guinier point	55	67