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- SASDFE5: Tetracycline repressor (class D), TetR(D), in complex with 5a,6-a... -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFE5
SampleTetracycline repressor (class D), TetR(D), in complex with 5a,6-anhydrotetracycline (ATc)
  • Tetracycline repressor (class D) (protein), TetR(D), Escherichia coli
  • 5a,6-anhydrotetracycline (other), ATc
Function / homology
Function and homology information


transcription cis-regulatory region binding / DNA-binding transcription factor activity / response to antibiotic / negative regulation of DNA-templated transcription / metal ion binding
Similarity search - Function
Tetracycline transcriptional regulator, TetR / Tetracycline repressor TetR, C-terminal / Tetracyclin repressor-like, C-terminal domain / DNA-binding HTH domain, TetR-type, conserved site / TetR-type HTH domain signature. / : / Tetracyclin repressor-like, C-terminal domain superfamily / Bacterial regulatory proteins, tetR family / DNA-binding HTH domain, TetR-type / TetR-type HTH domain profile. / Homeobox-like domain superfamily
Similarity search - Domain/homology
Tetracycline repressor protein class D
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Contact author
  • Sebastiaan Werten (Medical University of Innsbruck, Innsbruck, Austria)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2948
Type: atomic / Symmetry: p2
Comment: A dimer was generated from the PDB entry by applying crystallographic symmetry
Chi-square value: 1.760

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Sample

SampleName: Tetracycline repressor (class D), TetR(D), in complex with 5a,6-anhydrotetracycline (ATc)
Specimen concentration: 1.35-1.35 / Entity id: 1614 / 1615
BufferName: 50 mM Tris/HCl 150 mM NaCl 10 mM MgCl2 / pH: 8
Entity #1614Name: TetR(D) / Type: protein / Description: Tetracycline repressor (class D) / Formula weight: 23.259 / Num. of mol.: 2 / Source: Escherichia coli / References: UniProt: P0ACT4
Sequence: SRLNRESVID AALELLNETG IDGLTTRKLA QKLGIEQPTL YWHVKNKRAL LDALAVEILA RHHDYSLPAA GESWQSFLRN NAMSFRRALL RYRDGAKVHL GTRPDEKQYD TVETQLRFMT ENGFSLRDGL YAISAVSHFT LGAVLEQQEH TAALTDRPAA PDENLPPLLR ...Sequence:
SRLNRESVID AALELLNETG IDGLTTRKLA QKLGIEQPTL YWHVKNKRAL LDALAVEILA RHHDYSLPAA GESWQSFLRN NAMSFRRALL RYRDGAKVHL GTRPDEKQYD TVETQLRFMT ENGFSLRDGL YAISAVSHFT LGAVLEQQEH TAALTDRPAA PDENLPPLLR EALQIMDSDD GEQAFLHGLE SLIRGFEVQL TALLQIV
Entity #1615Name: ATc / Type: other / Description: 5a,6-anhydrotetracycline / Formula weight: 0.426 / Num. of mol.: 2
Sequence:
C22H22N2O7

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: Tetracycline repressor (class D), TetR(D), in complex with 5a,6-anhydrotetracycline (ATc)
Measurement date: Sep 23, 2013 / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0596 4.4578
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1157 /
MinMax
Q0.0938219 3.13464
P(R) point1 1157
R0 6.76
Result
Type of curve: merged
ExperimentalStandardPorod
MW42.6 kDa42.6 kDa48.2 kDa
Volume--77.16 nm3

P(R)GuinierGuinier error
Forward scattering, I07510 7682.32 15.74
Radius of gyration, Rg2.435 nm2.55 nm0.06

MinMax
D-6.76
Guinier point14 172

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