SASDFB4: Insulin glargine (Lantus ®), oligomeric mixture (Insulin glargine...

Basic information

Entry

Database: SASBDB / ID: SASDFB4

Sample

Insulin glargine (Lantus ®), oligomeric mixture

• Insulin glargine (Lantus ®) (protein)

• Citation: Date: 2019 Jul; Title: The quaternary structure of insulin glargine and glulisine under formulation conditions; Authors: Nagel N / Graewert M / Gao M / Heyse W / Jeffries C / Svergun D

Contact author

• Melissa Graewert (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

Sample

• Sample: Name: Insulin glargine (Lantus ®), oligomeric mixture / Specimen concentration: 3.64 mg/ml
• Buffer: Name: Lantus Formulation (30 µg Zinc cloride, 2.7 mg m-Cresol, 20 mg glycerol 85%); pH: 4

Entity #1569

Type: protein / Description: Insulin glargine (Lantus ®) / Formula weight: 6.051 / Num. of mol.: 6
Sequence:

GIVEQCCTSI CSLYQLENYC GFVNQHLCGS HLVEALYLVC GERGFFYTPK TRR

Experimental information

• Beam: Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 1.241 Å / Dist. spec. to detc.: 3.1 mm
• Detector: Name: Pilatus 2M

Scan

Title: Insulin glargine (Lantus ®), oligomeric mixture / Measurement date: May 23, 2017 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.1684	3.0281

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1014 /

	Min	Max
Q	0.242621	3.02813
P(R) point	1	1014
R	0	5.27

Result

Type of curve: single_conc /

	Experimental	Standard	Standard error
MW	35 kDa	35 kDa	3

	P(R)	Guinier	Guinier error
Forward scattering, I0	655.4	645.24	4.63
Radius of gyration, Rg	1.832 nm	1.78 nm	0.06

	Min	Max
D	-	5.27
Guinier point	28	206