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- SASDFA7: Receptor binding BinB protein of mosquito-larvicidal Binary toxi... -

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Basic information

Entry
Database: SASBDB / ID: SASDFA7
SampleReceptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O
  • Lysinibacillus Mosquito-larvicidal receptor binding component (protein), BinB, synthetic construct
Function / homologyInsecticidal crystal toxin / Insecticidal Crystal Toxin, P42 / : / toxin activity / Larvicidal toxin 51 kDa protein
Function and homology information
Biological speciessynthetic construct (others)
Contact author
  • Vinay Kumar (Bhabha Atomic Research Centre, Mumbai 400085, INDIA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3077
Type: dummy / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 0.385 / P-value: 0.086659
Model #3078
Type: atomic / Chi-square value: 0.523 / P-value: 0.010821

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Sample

SampleName: Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O
Specimen concentration: 3.3 mg/ml
BufferName: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O / pH: 7.5 / Comment: Protein maintained in 100% D2O
Entity #1690Name: BinB / Type: protein
Description: Lysinibacillus Mosquito-larvicidal receptor binding component
Formula weight: 53.457 / Num. of mol.: 1 / Source: synthetic construct / References: UniProt: B1HQ59
Sequence: MGSSHHHHHH SSGLVPRGSM CDSKDNSGVS EKCGKKFTNY PLNTTPTSLN YNLPEISKKF YNLKNKYSRN GYGLSKTEFP SSIENCPSNE YSIMYDNKDP RFLIRFLLDD GRYIIADRDD GEVFDEAPTY LDNNNHPIIS RHYTGEERQK FEQVGSGDYI TGEQFFQFYT ...Sequence:
MGSSHHHHHH SSGLVPRGSM CDSKDNSGVS EKCGKKFTNY PLNTTPTSLN YNLPEISKKF YNLKNKYSRN GYGLSKTEFP SSIENCPSNE YSIMYDNKDP RFLIRFLLDD GRYIIADRDD GEVFDEAPTY LDNNNHPIIS RHYTGEERQK FEQVGSGDYI TGEQFFQFYT QNKTRVLSNC RALDSRTILL STAKIFPIYP PASETQLTAF VNSSFYAAAI PQLPQTSLLE NIPEPTSLDD SGVLPKDAVR AVKGSALLPC IIVHDPNLNN SDKMKFNTYY LLEYKEYWHQ LWSQIIPAHQ TVKIQERTGI SEVVQNSMIE DLNMYIGADF GMLFYFRSSG FKEQITRGLN RPLSQTTTQL GERVEEMEYY NSNDLDVRYV KYALAREFTL KRVNGEIVKN WVAVDYRLAG IQSYPNAPIT NPLTLTKHTI IRCENSYDGH IFKTPLIFKN GEVIVKTNEE LIPKINQ

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Experimental information

BeamInstrument name: Dhruva Reactor, Bhabha Atomic Research Centre SANS-I facility
City: Mumbai / : India / Type of source: neutron source / Wavelength: 0.52 Å / Dist. spec. to detc.: 2 mm
DetectorName: Ingenuously built / Type: 1 m long He3 position sensitive detector / Pixsize x: 12 mm
Scan
Title: Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O
Measurement date: Apr 24, 2019 / Storage temperature: 25 °C / Cell temperature: 25 °C / Unit: 1/A /
MinMax
Q0.0162 0.1991
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 100 /
MinMax
Q0.01619 0.1991
P(R) point1 100
R0 99.2
Result
Type of curve: single_conc
Comments: Experimental Molecular weight Value estimated using I(0) values from GNOM analysis. Intensity has been normalized to protein concentration of 1 mg/ml. Actual sample used was 3.3 mg/ml.
ExperimentalStandard
MW61.9 kDa62.3 kDa

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.04353 0.002346 0.045 0.002
Radius of gyration, Rg3.16 nm0.283 3.28 nm0.55

MinMax
D-9.92
Guinier point1 13

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