+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDF97 |
---|---|
試料 | Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O
|
生物種 | synthetic construct (人工物) |
登録者 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-Data source
SASBDBのページ | SASDF97 |
---|
-モデル
モデル #3082 | タイプ: dummy / ダミー原子の半径: 1.90 A / 対称性: P1 コメント: dBinB component is fully 'contrast matched' with 100% D2O, カイ2乗値: 0.318 / P-value: 0.086659 |
---|---|
モデル #3084 | タイプ: atomic / カイ2乗値: 0.348 / P-value: 0.000320 |
-試料
試料 | 名称: Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O 試料濃度: 1 mg/ml / Entity id: 1691 / 1692 |
---|---|
バッファ | 名称: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O / pH: 7.5 / コメント: SANS experiment were carried out in 100% D2O |
要素 #1691 | 名称: Cqm1 / タイプ: protein / 記述: Binary toxin receptor Cqm1 protein / 分子量: 64.665 / 分子数: 1 / 由来: synthetic construct 配列: MHHHHHHSSG VDLGTENLYF QSMREPDSKD WYQHATFYQI YPRSFLDSNG DGIGDLAGIT SKMKYLADIG IDATWLSPPF KSPLKDFGYD VSDFYAIQPE YGNLTDFDKL VEESHKNGIK LMLDFIPNHS SDQHEWFVKS VVRDPEYSDF YVWRPPATGG GPPNNWISVF ...配列: MHHHHHHSSG VDLGTENLYF QSMREPDSKD WYQHATFYQI YPRSFLDSNG DGIGDLAGIT SKMKYLADIG IDATWLSPPF KSPLKDFGYD VSDFYAIQPE YGNLTDFDKL VEESHKNGIK LMLDFIPNHS SDQHEWFVKS VVRDPEYSDF YVWRPPATGG GPPNNWISVF GGSAWTYNQA RGEYYLHQFT PQQPDLNYRN PKVLAEMTKM LFFWLDRGVD GFRLDAINHM FEDEQFRDEP LSGWGQPGEY DSLDHIYTKD IPDVYDVVYN WRDQMDKYSA EKGRTIILMT EAYSSIEGTM LYYESADRKR QGAHMPFNFQ LIYDFKKEQN AVGLKSSIDW WMNNMPARHT PSWVAGSHDH SRVASRVGLD RVDQVMTLLH TLPGTSITYY GEEVAMQDFK EAQQFDNRDP NRTPMQWDSS TSAGFSTNTN TWLRVHPDYA RYNVDVMQKN PQSTFHHFQH LTKLRRHRTM QSGEYVHKTV GTKVYALLRE LRGEDSFLTV LNMAGAEDTV DLGDFVNLPQ KMRVEVAQPN SKSKAGNEVD ISKLTLGPYD SVVLRATVSS A |
要素 #1692 | 名称: dBinB / タイプ: protein 記述: Deuterated BinB component of mosquito-larvicidal Binary toxin 分子量: 56.4 / 分子数: 1 / 由来: synthetic construct 配列: MGSSHHHHHH SSGLVPRGSM CDSKDNSGVS EKCGKKFTNY PLNTTPTSLN YNLPEISKKF YNLKNKYSRN GYGLSKTEFP SSIENCPSNE YSIMYDNKDP RFLIRFLLDD GRYIIADRDD GEVFDEAPTY LDNNNHPIIS RHYTGEERQK FEQVGSGDYI TGEQFFQFYT ...配列: MGSSHHHHHH SSGLVPRGSM CDSKDNSGVS EKCGKKFTNY PLNTTPTSLN YNLPEISKKF YNLKNKYSRN GYGLSKTEFP SSIENCPSNE YSIMYDNKDP RFLIRFLLDD GRYIIADRDD GEVFDEAPTY LDNNNHPIIS RHYTGEERQK FEQVGSGDYI TGEQFFQFYT QNKTRVLSNC RALDSRTILL STAKIFPIYP PASETQLTAF VNSSFYAAAI PQLPQTSLLE NIPEPTSLDD SGVLPKDAVR AVKGSALLPC IIVHDPNLNN SDKMKFNTYY LLEYKEYWHQ LWSQIIPAHQ TVKIQERTGI SEVVQNSMIE DLNMYIGADF GMLFYFRSSG FKEQITRGLN RPLSQTTTQL GERVEEMEYY NSNDLDVRYV KYALAREFTL KRVNGEIVKN WVAVDYRLAG IQSYPNAPIT NPLTLTKHTI IRCENSYDGH IFKTPLIFKN GEVIVKTNEE LIPKINQ |
-実験情報
ビーム | 設備名称: Dhruva Reactor, Bhabha Atomic Research Centre SANS-I facility 地域: Mumbai / 国: India / 線源: neutron source / 波長: 0.52 Å / スペクトロメータ・検出器間距離: 2 mm | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: Ingenuously built / タイプ: 1 m long He3 position sensitive detector / Pixsize x: 12 mm | ||||||||||||||||||||||||||||||
スキャン | タイトル: Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O 測定日: 2019年4月23日 / 保管温度: 25 °C / セル温度: 25 °C / 単位: 1/A /
| ||||||||||||||||||||||||||||||
距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 100 /
| ||||||||||||||||||||||||||||||
結果 | カーブのタイプ: single_conc コメント: This data is for a stable complex of receptor Cqm1 protein and deuterated BinB component of mosquito-larvicidal Binary (BinAB) toxin. Deuterated BinB (dBinB) protein was synthesized ...コメント: This data is for a stable complex of receptor Cqm1 protein and deuterated BinB component of mosquito-larvicidal Binary (BinAB) toxin. Deuterated BinB (dBinB) protein was synthesized using E. coli based protein expression system grown in M9+ medium in D2O after three step adaption. The extent of deuteration for dBinB was estimated from contrast (p − s)2 values to be ~77%. Theoretical molecular weight of the dBinB protein includes 77% of D atoms. SANS analysis (DAMMIN and CRYSON fit) suggest that deuterated BinB (dBinB), expectedly, is fully matched out against the used solvent (100% D2O) and only monomer of Cqm1 is modelled from SANS experiments
|