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- SASDF95: Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA... -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDF95
SamplePseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex
  • Uncharacterized protein (protein), Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
  • DNA Duplex (DNA)
Function / homologyToxin-antitoxin system, antidote protein, HigA / Helix-turn-helix / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / Cro/C1-type helix-turn-helix domain / Lambda repressor-like, DNA-binding domain superfamily / DNA binding / HTH cro/C1-type domain-containing protein
Function and homology information
Biological speciesPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Contact author
  • Guangfeng Liu (National Center for Protein Science Shanghai, Shanghai, China)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2944
Type: dummy / Radius of dummy atoms: 3.00 A / Symmetry: p1 / Chi-square value: 1.053 / P-value: 0.019260
Model #2945
Type: atomic / Chi-square value: 6.229

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Sample

SampleName: Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex
Specimen concentration: 1.00-5.00 / Entity id: 1603 / 1605
BufferName: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF
pH: 8
Entity #1603Type: protein / Description: Uncharacterized protein / Formula weight: 11.248 / Num. of mol.: 2
Source: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
References: UniProt: Q9HVC1
Sequence:
MATNGMRPIH PGEILRDEFL MEFDISPAAL ARALKVSAPT VNDIVREQRG ISADMAIRLG RYFDTSAQFW MNLQSEYSLA TAYAANGKQI EHEIEPLLAH G
Entity #1605Type: DNA / Description: DNA Duplex / Formula weight: 10.006 / Num. of mol.: 2
Sequence:
TGAAGTTAAC GCTTAACGTT AAGGGTTAAT GA

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Experimental information

BeamInstrument name: Shanghai Synchrotron Radiation Facility (SSRF) BL19U2
City: Shanghai / 国: China / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex
Measurement date: Sep 19, 2018 / Storage temperature: 10 °C / Cell temperature: 23 °C / Exposure time: 1 sec. / Number of frames: 20 / Unit: 1/A /
MinMax
Q0.007 0.3348
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 651 /
MinMax
Q0.0198541 0.27269
P(R) point1 651
R0 98.3
Result
Type of curve: merged
ExperimentalStandardStandard errorPorod
MW73.8 kDa73.8 kDa1.2 -
Volume---81.1 nm3

P(R)GuinierGuinier error
Forward scattering, I080.36 80.36 0.17
Radius of gyration, Rg2.98 nm2.935 nm0.008

MinMax
D-9.83
Guinier point34 95

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