SASDF66: DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 4.5 ...

Basic information

Entry

Database: SASBDB / ID: SASDF66

Sample

DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 300 mM NaCl

• 80bp_DNA Forward (DNA), Escherichia coli
• 80bp_DNA Reverse (DNA), Escherichia coli K-12
• DNA-binding protein HU-alpha (protein), Escherichia coli (strain K12)

Function / homology

Function:

HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol

Domain/homology:

Histone-like DNA-binding protein, conserved site / Bacterial histone-like DNA-binding proteins signature. / Histone-like DNA-binding protein / Bacterial DNA-binding protein / bacterial (prokaryotic) histone like domain / Integration host factor (IHF)-like DNA-binding domain superfamily

Component:

DNA-binding protein HU-alpha

• Biological species: Escherichia coli (E. coli); Escherichia coli K-12 (bacteria); Escherichia coli (strain K12) (bacteria)

Contact author

• Soumya G Remesh (Lawrence Berkeley National laboratory)

Models

Sample

• Sample: Name: DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 300 mM NaCl; Entity id: 1622 / 1623 / 1624
• Buffer: Name: 10 mM sodium acetate, 300 mM NaCl / pH: 4.5

Entity #1622

Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli
Sequence:

AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT

Entity #1623

Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12
Sequence:

TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA

Entity #1624

Type: protein / Description: DNA-binding protein HU-alpha / Formula weight: 9.535 / Source: Escherichia coli (strain K12) / References: UniProt: P0ACF0
Sequence:

MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKEGD AVQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK

Experimental information

• Beam: Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS); City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
• Detector: Name: Pilatus3 X 2M / Pixsize x: 172 mm

Scan

Title: DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 300 mM NaCl
Measurement date: Jun 1, 2018 / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 300 / Unit: 1/A /

	Min	Max
Q	0.012	0.3503

• Result: Experimental MW: 300 kDa / Rg from PR: 2 nm / Type of curve: single_conc; Comments: SAXS profile corresponds to a protein-DNA complex between bacterial nucleoid associated protein HUalpha and 80bp DNA. Under these conditions, the complex forms lamellar structures visible as Bragg Diffraction peaks.