SASDF62: Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase d...

Basic information

Entry

Database: SASBDB / ID: SASDF62

Sample

Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment, at 1.0 mg/ml

• Dosage compensation regulator (protein), MLE, Drosophila melanogaster
• roX2 stem-loop 7, 18-mer fragment (RNA), SL7-18mer, synthetic construct

Function / homology

Function:

RIP-mediated NFkB activation via ZBP1 / X chromosome located dosage compensation complex, transcription activating / 3'-5' DNA/RNA helicase activity / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / male courtship behavior, veined wing generated song production / regulation of cytoplasmic translation / regulatory region RNA binding / sex-chromosome dosage compensation / PKR-mediated signaling / MSL complex / dosage compensation by hyperactivation of X chromosome / positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity / polytene chromosome / 3'-5' RNA helicase activity / regulation of mRNA processing / axon extension / lncRNA binding / 3'-5' DNA helicase activity / DNA duplex unwinding / nuclear chromosome / positive regulation of heterochromatin formation / X chromosome / DNA helicase activity / helicase activity / determination of adult lifespan / double-stranded RNA binding / chromosome / double-stranded DNA binding / RNA helicase activity / RNA helicase / ribonucleoprotein complex / chromatin binding / chromatin / nucleolus / ATP hydrolysis activity / positive regulation of transcription by RNA polymerase II / RNA binding / ATP binding / nucleus / cytosol

Domain/homology:

DHX9, first double-stranded RNA binding domain / DHX9, second double-stranded RNA binding domain / DHX9, DEXH-box helicase domain / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase-associated domain / Helicase associated domain (HA2), winged-helix / Helicase associated domain (HA2) Add an annotation / Double-stranded RNA binding motif / Double-stranded RNA binding motif / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase

Component:

Dosage compensation regulator

• Biological species: Drosophila melanogaster (fruit fly); synthetic construct (others)

Contact author

• Po-chia Chen (EMBL, European Molecular Biology Laboratory (EMBL) - Heidelberg, Heidelberg, Germany)

Models

Sample

• Sample: Name: Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment, at 1.0 mg/ml; Specimen concentration: 1 mg/ml / Entity id: 1422 / 1424
• Buffer: Name: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT / pH: 6.5

Entity #1422

Name: MLE / Type: protein / Description: Dosage compensation regulator / Formula weight: 28.549 / Num. of mol.: 1 / Source: Drosophila melanogaster / References: UniProt: P24785
Sequence:

GAMDIKSFLY QFCAKSQIEP KFDIRQTGPK NRQRFLCEVR VEPNTYIGVG NSTNKKDAEK NACRDFVNYL VRVGKLNTND VPADAGASGG GPRTGLEGAG MAGGSGQQKR VFDGQSGPQD LGEAYRPLNH DGGDGGNRYS VIDRIQEQRD MNEAEAFDVN AAIHGNWTIE NAKERLNIYK QTNNIRDDYK YTPVGPEHAR SFLAELSIYV PALNRTVTAR ESGSNKKSAS KSCALSLVRQ LFHLNVIEPF SGTLKKKKD

Entity #1424

Name: SL7-18mer / Type: RNA / Description: roX2 stem-loop 7, 18-mer fragment / Formula weight: 11.592 / Num. of mol.: 1 / Source: synthetic construct
Sequence:

AGACGUGUAA AAUGUUGCUC CGCGCAUUUU GCAAUG

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.867 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment, at 1.0 mg/ml
Measurement date: Nov 29, 2016 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /

	Min	Max
Q	0.0348	4.9439

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 406 /

	Min	Max
Q	0.0960405	2.0041
P(R) point	1	406
R	0	13.3

Result

Type of curve: single_conc /

	Experimental	Porod
MW	7.3 kDa	-
Volume	-	25 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	41.25	40.73	0.084
Radius of gyration, Rg	3.34 nm	3.11 nm	0.31

	Min	Max
D	-	13.3
Guinier point	14	73