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- SASDF55: Leukocidin (LukGH) from Staphylococcus aureus in complex with a n... -

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Entry
Database: SASBDB / ID: SASDF55
SampleLeukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody
  • Leukocidin G (protein), LukG, Staphylococcus aureus
  • Leukocidin H (protein), LukH, Staphylococcus aureus
  • LukGH neutralizing antibody (protein), Fab, Homo sapiens
Biological speciesStaphylococcus aureus (bacteria)
Homo sapiens (human)

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Sample

SampleName: Leukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody
Specimen concentration: 0.40-4.60 / Entity id: 1577 / 1578 / 1579
BufferName: 20 mM Hepes, 300 mM NaCl, 1 mM MgCl2 / pH: 7.5
Entity #1577Name: LukG / Type: protein / Description: Leukocidin G / Formula weight: 35.573 / Num. of mol.: 1 / Source: Staphylococcus aureus
Sequence: KINSEIKQVS EKNLDGDTKM YTRTATTSDS QKNITQSLQF NFLTEPNYDK ETVFIKAKGT IGSGLRILDP NGYWNSTLRW PGSYSVSIQN VDDNNNTNVT DFAPKNQDES REVKYTYGYK TGGDFSINRG GLTGNITKES NYSETISYQQ PSYRTLLDQS TSHKGVGWKV ...Sequence:
KINSEIKQVS EKNLDGDTKM YTRTATTSDS QKNITQSLQF NFLTEPNYDK ETVFIKAKGT IGSGLRILDP NGYWNSTLRW PGSYSVSIQN VDDNNNTNVT DFAPKNQDES REVKYTYGYK TGGDFSINRG GLTGNITKES NYSETISYQQ PSYRTLLDQS TSHKGVGWKV EAHLINNMGH DHTRQLTNDS DNRTKSEIFS LTRNGNLWAK DNFTPKDKMP VTVSEGFNPE FLAVMSHDKK DKGKSQFVVH YKRSMDEFKI DWNRHGFWGY WSGENHVDKK EEKLSALYEV DWKTHNVKFV KVLNDNEKK
Entity #1578Name: LukH / Type: protein / Description: Leukocidin H / Formula weight: 37.619 / Num. of mol.: 1 / Source: Staphylococcus aureus
Sequence: NSAHKDSQDQ NKKEHVDKSQ QKDKRNVTNK DKNSTAPDDI GKNGKITKRT ETVYDEKTNI LQNLQFDFID DPTYDKNVLL VKKQGSIHSN LKFESHKEEK NSNWLKYPSE YHVDFQVKRN RKTEILDQLP KNKISTAKVD STFSYSSGGK FDSTKGIGRT SSNSYSKTIS ...Sequence:
NSAHKDSQDQ NKKEHVDKSQ QKDKRNVTNK DKNSTAPDDI GKNGKITKRT ETVYDEKTNI LQNLQFDFID DPTYDKNVLL VKKQGSIHSN LKFESHKEEK NSNWLKYPSE YHVDFQVKRN RKTEILDQLP KNKISTAKVD STFSYSSGGK FDSTKGIGRT SSNSYSKTIS YNQQNYDTIA SGKNNNWHVH WSVIANDLKY GGEVKNRNDE LLFYRNTRIA TVENPELSFA SKYRYPALVR SGFNPEFLTY LSNEKSNEKT QFEVTYTRNQ DILKNRPGIH YAPPILEKNK DGQRLIVTYE VDWKNKTVKV VDKYSDDNKP YKEG
Entity #1579Name: Fab / Type: protein / Description: LukGH neutralizing antibody / Formula weight: 47.441 / Num. of mol.: 1 / Source: Homo sapiens
Sequence: ELQLQESGPG LVKPSETLSL TCTVSGGSIS SGSYYWDWIR QPPGKGLEWI GNIYKSGSTY YNPSLKSRVT ISVDTSKNQF SLKLSSVTAA DTAVYYCARE RGMHYMDVWG KGTTVTVSSA STKGPSVFPL APSSKSTSGG TAALGCLVKD YFPEPVTVSW NSGALTSGVH ...Sequence:
ELQLQESGPG LVKPSETLSL TCTVSGGSIS SGSYYWDWIR QPPGKGLEWI GNIYKSGSTY YNPSLKSRVT ISVDTSKNQF SLKLSSVTAA DTAVYYCARE RGMHYMDVWG KGTTVTVSSA STKGPSVFPL APSSKSTSGG TAALGCLVKD YFPEPVTVSW NSGALTSGVH TFPAVLQSSG LYSLSSVVTV PSSSLGTQTY ICNVNHKPSN TKVDKKVEPK SCDKTHTDIQ MTQSPSSLSA SVGDRVTITC RASQSINSYL NWYQQKPGKA PKLLIYAASS LQSGVPSRFS GSGSGTDFTL TISSLQPEDF ATYYCQQQFD PPFTFGGGTK VEIKRTVAAP SVFIFPPSDE QLKSGTASVV CLLNNFYPRE AKVQWKVDNA LQSGNSQESV TEQDSKDSTY SLSSTLTLSK ADYEKHKVYA CEVTHQGLSS PVTKSFNRGE C

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
ScanTitle: Leukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody
Measurement date: Sep 17, 2017 / Storage temperature: 15 °C / Cell temperature: 10 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0468 3.7737
ResultType of curve: merged
ExperimentalStandardStandard errorPorod
MW124 kDa90 kDa15 95 kDa
Volume---152 nm3

P(R)GuinierGuinier error
Forward scattering, I05749 5745.48 16.43
Radius of gyration, Rg4.86 nm4.73 nm0.12

MinMax
D-16
Guinier point9 104

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