SASDF49: Double-stranded RNA-binding protein Staufen homolog 1 - RNA bindi...

Basic information

Entry

Database: SASBDB / ID: SASDF49

Sample

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long

• Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 (protein), STAU1-RBD34, Homo sapiens
• ADP-ribosylation factor1 - long (RNA), ARF1 RNA - long, Homo sapiens

Function / homology

Function:

lncRNA-mediated post-transcriptional gene silencing / anterograde dendritic transport of messenger ribonucleoprotein complex / messenger ribonucleoprotein complex / modification of postsynaptic structure / intracellular mRNA localization / protein localization to synapse / protein phosphatase 1 binding / positive regulation by virus of viral protein levels in host cell / microtubule associated complex / germ cell development / positive regulation of viral genome replication / rough endoplasmic reticulum / dendrite cytoplasm / positive regulation of long-term synaptic potentiation / cytoplasmic stress granule / cytoplasmic ribonucleoprotein granule / double-stranded RNA binding / cellular response to oxidative stress / cell body / neuron projection / mRNA binding / neuronal cell body / glutamatergic synapse / dendrite / endoplasmic reticulum / RNA binding / extracellular exosome / membrane / plasma membrane / cytosol / cytoplasm

Domain/homology:

Staufen 1, third double-stranded RNA binding domain / Staufen, C-terminal / Staufen C-terminal domain / Double-stranded RNA binding motif / Double-stranded RNA binding motif / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain

Component:

Double-stranded RNA-binding protein Staufen homolog 1

• Biological species: Homo sapiens (human)

Contact author

• Tomas Klumpler (CEITEC - Central European Institute of Technology, Masaryk University)

Models

Sample

• Sample: Name: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long; Specimen concentration: 5 mg/ml / Entity id: 1743 / 1745
• Buffer: Name: 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol; pH: 6.8

Entity #1743

Name: STAU1-RBD34 / Type: protein
Description: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4
Formula weight: 19.733 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: O95793
Sequence:

GSHMKSEISQ VFEIALKRNL PVNFEVARES GPPHMKNFVT KVSVGEFVGE GEGKSKKISK KNAAIAVLEE LKKLPPLPAV ERVKPRIKKK TKPIVKPQTS PEYGQGINPI SRLAQIQQAK KEKEPEYTLL TERGLPRRRE FVMQVKVGNH TAEGTGTNKK VAKRNAAENM LEILGFK

Entity #1745

Name: ARF1 RNA - long / Type: RNA / Description: ADP-ribosylation factor1 - long / Formula weight: 16.137 / Num. of mol.: 1 / Source: Homo sapiens
Sequence:

GGCGAGUGCC AGAAGCUGUC UCCUUCGGGA GGCAGUUUCU GGUACUCGCC

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1025 Å
• Detector: Name: Pilatus 2M

Scan

Title: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long
Measurement date: Sep 20, 2018 / Cell temperature: 15 °C / Exposure time: 3 sec. / Number of frames: 1 / Unit: 1/A /

	Min	Max
Q	0.0206	0.3001

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 253 /

	Min	Max
Q	0.0205826	0.300117
P(R) point	1	253
R	0	69.75

Result

Type of curve: single_conc /

	Experimental	Porod
MW	34 kDa	33 kDa
Volume	-	53 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.009973	0.01024	7.8E-5
Radius of gyration, Rg	2.46 nm	2.536 nm	0.063

	Min	Max
D	-	6.97
Guinier point	1	28