SASDF22: Ternary complex of FcRn ectodomain, FcRn binding optimised human ...

Basic information

Entry

Database: SASBDB / ID: SASDF22

Sample

Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

• somapacitan (protein), Homo sapiens
• FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A (protein), HSAopt, Homo sapiens
• neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag (protein), FcRn ECD beta, Homo sapiens
• neonatal Fc receptor ectodomain alpha-chain (protein), FcRn ECD alpha, Homo sapiens

Function / homology

Function:

growth hormone activity / growth hormone receptor complex / bone maturation / prolactin receptor binding / IgG immunoglobulin transcytosis in epithelial cells mediated by FcRn immunoglobulin receptor / positive regulation of growth / animal organ development / cell surface receptor signaling pathway via STAT / regulation of glutamate receptor signaling pathway / regulation of calcium ion import across plasma membrane / aspartic-type endopeptidase inhibitor activity / glycosaminoglycan binding / exogenous protein binding / positive regulation of multicellular organism growth / Ciprofloxacin ADME / cellular response to calcium ion starvation / positive regulation of D-glucose transmembrane transport / negative regulation of interleukin-17 production / type 5 metabotropic glutamate receptor binding / negative regulation of dendritic spine maintenance / cupric ion binding / regulation of potassium ion transmembrane transport / enterobactin binding / positive regulation of insulin-like growth factor receptor signaling pathway / growth hormone receptor binding / Heme biosynthesis / HDL remodeling / IgG binding / negative regulation of mitochondrial depolarization / Prednisone ADME / growth hormone receptor signaling pathway / negative regulation of calcineurin-NFAT signaling cascade / Heme degradation / negative regulation of T cell receptor signaling pathway / negative regulation of interleukin-2 production / Aspirin ADME / Prolactin receptor signaling / negative regulation of amyloid-beta formation / negative regulation of activated T cell proliferation / cuprous ion binding / antioxidant activity / cell surface receptor signaling pathway via JAK-STAT / negative regulation of type II interferon production / positive regulation of protein targeting to membrane / Synthesis, secretion, and deacylation of Ghrelin / toxic substance binding / growth hormone receptor signaling pathway via JAK-STAT / beta-2-microglobulin binding / Scavenging of heme from plasma / Growth hormone receptor signaling / Recycling of bile acids and salts / side of membrane / positive regulation of tyrosine phosphorylation of STAT protein / inclusion body / cellular response to copper ion / neuron projection maintenance / cellular response to starvation / response to nutrient levels / platelet alpha granule lumen / negative regulation of protein phosphorylation / molecular function activator activity / cytokine activity / endosome lumen / fatty acid binding / positive regulation of protein localization to plasma membrane / Post-translational protein phosphorylation / positive regulation of receptor signaling pathway via JAK-STAT / growth factor activity / positive regulation of MAP kinase activity / molecular condensate scaffold activity / protein destabilization / protein homooligomerization / Cytoprotection by HMOX1 / hormone activity / terminal bouton / cytokine-mediated signaling pathway / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / cellular response to amyloid-beta / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of neuron apoptotic process / cellular response to xenobiotic stimulus / pyridoxal phosphate binding / Platelet degranulation / response to estradiol / signaling receptor activity / amyloid-beta binding / protein-folding chaperone binding / microtubule binding / protease binding / nuclear membrane / blood microparticle / response to oxidative stress / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / learning or memory / endosome membrane / immune response / membrane raft / copper ion binding / endoplasmic reticulum lumen / external side of plasma membrane

Domain/homology:

Somatotropin / Somatotropin/prolactin / Somatotropin hormone, conserved site / Somatotropin hormone family / Somatotropin, prolactin and related hormones signature 1. / Somatotropin, prolactin and related hormones signature 2. / Major prion protein N-terminal domain / Major prion protein bPrPp - N terminal / Prion protein signature 1. / Prion protein signature 2. / Prion protein / Major prion protein / Prion/Doppel protein, beta-ribbon domain / Prion/Doppel beta-ribbon domain superfamily / Prion/Doppel alpha-helical domain / Serum albumin/Alpha-fetoprotein/Afamin / ALB/AFP/VDB / Serum albumin, N-terminal / Serum albumin, conserved site / Serum albumin-like / Serum albumin family / Albumin domain signature. / Albumin domain profile. / serum albumin / Four-helical cytokine-like, core / Class I Histocompatibility antigen, domains alpha 1 and 2 / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold

Component:

Somatotropin / Albumin / IgG receptor FcRn large subunit p51 / Major prion protein

• Biological species: Homo sapiens (human)

Contact author

• Eva Johansson (Novo Nordisk A/S)

Models

Sample

• Sample: Name: Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.; Specimen concentration: 4.5 mg/ml / Entity id: 1462 / 1464 / 1467 / 1475
• Buffer: Name: 100 mM MES, 100 mM NaCl / pH: 6.5

Entity #1462

Type: protein / Description: somapacitan / Formula weight: 22.119 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P01241
Sequence:

FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS CVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

Entity #1464

Name: HSAopt / Type: protein
Description: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A
Formula weight: 66.373 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P02768
Sequence:

DAHKSEVAHR FKDLGEENFK ALVLIAFAQY LQQCPFEDHV KLVNEVTEFA KTCVADESAE NCDKSLHTLF GDKLCTVATL RETYGEMADC CAKQEPERNE CFLQHKDDNP NLPRLVRPEV DVMCTAFHDN EETFLKKYLY EIARRHPYFY APELLFFAKR YKAAFTECCQ AADKAACLLP KLDELRDEGK ASSAKQRLKC ASLQKFGERA FKAWAVARLS QRFPKAEFAE VSKLVTDLTK VHTECCHGDL LECADDRADL AKYICENQDS ISSKLKECCE KPLLEKSHCI AEVENDEMPA DLPSLAADFV ESKDVCKNYA EAKDVFLGMF LYEYARRHPD YSVVLLLRLA KTYETTLEKC CAAADPHECY AKVFDEFKPL VEEPQNLIKQ NCELFEQLGE YKFQNALLVR YTKKVPQMSA PTLVEVSRNL GKVGSKCCKH PEAKRMPCAE DYLSVVLNQL CVLHEKTPVS DRVTKCCTES LVNRRPCFSA LEVDETYVPK EFNAGTFTFH ADICTLSEKE RQIKKQTALV ELVKHKPKAT KEQLKAAMDD FAAFVEKCCK ADDKETCFAE EGKKLVAASQ AALGL

Entity #1467

Name: FcRn ECD beta / Type: protein
Description: neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag
Formula weight: 12.554 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P61768
Sequence:

IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDMH HHHHH

Entity #1475

Name: FcRn ECD alpha / Type: protein / Description: neonatal Fc receptor ectodomain alpha-chain / Formula weight: 30.375 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P55899
Sequence:

AESHLSLLYH LTAVSSPAPG TPAFWVSGWL GPQQYLSYNS LRGEAEPCGA WVWENQVSWY WEKETTDLRI KEKLFLEAFK ALGGKGPYTL QGLLGCELGP DNTSVPTAKF ALNGEEFMNF DLKQGTWGGD WPEALAISQR WQQQDKAANK ELTFLLFSCP HRLREHLERG RGNLEWKEPP SMRLKARPSS PGFSVLTCSA FSFYPPELQL RFLRNGLAAG TGQGDFGPNS DGSFHASSSL TVKSGDEHHY CCIVQHAGLA QPLRVELESP AKSS

Experimental information

• Beam: Instrument name: MAX IV I911-4 / City: Lund / 国: Sweden / Type of source: X-ray synchrotron / Wavelength: 0.1 Å
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.
Measurement date: May 11, 2015 / Storage temperature: 25 °C / Cell temperature: 25 °C / Exposure time: 120 sec. / Number of frames: 1 / Unit: 1/A /

	Min	Max
Q	0.0094	0.4379

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 306 /

	Min	Max
Q	0.01062	0.1906
P(R) point	3	308
R	0	146.6

Result

Type of curve: single_conc /

	Experimental	Porod
MW	130 kDa	132 kDa
Volume	-	227 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.4737	0.4742	0.001
Radius of gyration, Rg	4.2 nm	4.15 nm	0.01

	Min	Max
D	-	14.66
Guinier point	3	38