SASDF22: Ternary complex of FcRn ectodomain, FcRn binding optimised human ...

Basic information

Entry

Database: SASBDB / ID: SASDF22

Sample

Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

• somapacitan (protein), Homo sapiens
• FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A (protein), HSAopt, Homo sapiens
• neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag (protein), FcRn ECD beta, Homo sapiens
• neonatal Fc receptor ectodomain alpha-chain (protein), FcRn ECD alpha, Homo sapiens

Function / homology

Function:

growth hormone receptor complex / : / bone maturation / prolactin receptor binding / cellular response to xenobiotic stimulus => GO:0071466 / IgG immunoglobulin transcytosis in epithelial cells mediated by FcRn immunoglobulin receptor / : / positive regulation of growth / animal organ development / : / positive regulation of activation of Janus kinase activity / regulation of glutamate receptor signaling pathway / regulation of calcium ion import across plasma membrane / cellular response to calcium ion starvation / exogenous protein binding / positive regulation of multicellular organism growth / aspartic-type endopeptidase inhibitor activity / glycosaminoglycan binding / Ciprofloxacin ADME / negative regulation of interleukin-17 production / regulation of potassium ion transmembrane transport / positive regulation of glucose transmembrane transport / negative regulation of dendritic spine maintenance / type 5 metabotropic glutamate receptor binding / cupric ion binding / enterobactin binding / Heme biosynthesis / IgG binding / positive regulation of insulin-like growth factor receptor signaling pathway / HDL remodeling / growth hormone receptor binding / negative regulation of mitochondrial depolarization / Prednisone ADME / negative regulation of calcineurin-NFAT signaling cascade / Heme degradation / growth hormone receptor signaling pathway / negative regulation of interleukin-2 production / negative regulation of T cell receptor signaling pathway / Aspirin ADME / Prolactin receptor signaling / activation of protein kinase activity / negative regulation of amyloid-beta formation / negative regulation of activated T cell proliferation / antioxidant activity / cell surface receptor signaling pathway via JAK-STAT / : / negative regulation of type II interferon production / positive regulation of protein targeting to membrane / toxic substance binding / Synthesis, secretion, and deacylation of Ghrelin / Scavenging of heme from plasma / beta-2-microglobulin binding / Growth hormone receptor signaling / Recycling of bile acids and salts / positive regulation of tyrosine phosphorylation of STAT protein / cellular response to starvation / neuron projection maintenance / response to nutrient levels / negative regulation of protein phosphorylation / platelet alpha granule lumen / cytokine activity / endosome lumen / fatty acid binding / positive regulation of protein localization to plasma membrane / Post-translational protein phosphorylation / positive regulation of receptor signaling pathway via JAK-STAT / growth factor activity / positive regulation of MAP kinase activity / protein homooligomerization / negative regulation of DNA-binding transcription factor activity / terminal bouton / hormone activity / Cytoprotection by HMOX1 / cytokine-mediated signaling pathway / cellular response to amyloid-beta / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / positive regulation of peptidyl-tyrosine phosphorylation / pyridoxal phosphate binding / response to estradiol / Platelet degranulation / signaling receptor activity / amyloid-beta binding / protein-folding chaperone binding / nuclear membrane / blood microparticle / response to oxidative stress / protease binding / learning or memory / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / endosome membrane / immune response / membrane raft / copper ion binding / external side of plasma membrane / endoplasmic reticulum lumen / dendrite / calcium ion binding / protein-containing complex binding / negative regulation of apoptotic process / Golgi apparatus

Domain/homology:

Somatotropin / Somatotropin/prolactin / Somatotropin hormone, conserved site / Somatotropin hormone family / Somatotropin, prolactin and related hormones signature 1. / Somatotropin, prolactin and related hormones signature 2. / Prion protein signature 1. / Prion protein signature 2. / Major prion protein N-terminal domain / Major prion protein bPrPp - N terminal / Prion protein / Major prion protein / Prion/Doppel protein, beta-ribbon domain / Prion/Doppel beta-ribbon domain superfamily / Prion/Doppel alpha-helical domain / Serum albumin/Alpha-fetoprotein/Afamin / ALB/AFP/VDB / Serum albumin, N-terminal / Serum albumin, conserved site / Serum albumin-like / Serum albumin family / Albumin domain signature. / Albumin domain profile. / serum albumin / Four-helical cytokine-like, core / Class I Histocompatibility antigen, domains alpha 1 and 2 / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold

Component:

Somatotropin / Albumin / IgG receptor FcRn large subunit p51 / Major prion protein

• Biological species: Homo sapiens (human)

Contact author

• Eva Johansson (Novo Nordisk A/S)

Models

Sample

• Sample: Name: Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.; Specimen concentration: 4.5 mg/ml / Entity id: 1462 / 1464 / 1467 / 1475
• Buffer: Name: 100 mM MES, 100 mM NaCl / pH: 6.5

Entity #1462

Type: protein / Description: somapacitan / Formula weight: 22.119 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P01241
Sequence:

FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS CVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

Entity #1464

Name: HSAopt / Type: protein
Description: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A
Formula weight: 66.373 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P02768
Sequence:

DAHKSEVAHR FKDLGEENFK ALVLIAFAQY LQQCPFEDHV KLVNEVTEFA KTCVADESAE NCDKSLHTLF GDKLCTVATL RETYGEMADC CAKQEPERNE CFLQHKDDNP NLPRLVRPEV DVMCTAFHDN EETFLKKYLY EIARRHPYFY APELLFFAKR YKAAFTECCQ AADKAACLLP KLDELRDEGK ASSAKQRLKC ASLQKFGERA FKAWAVARLS QRFPKAEFAE VSKLVTDLTK VHTECCHGDL LECADDRADL AKYICENQDS ISSKLKECCE KPLLEKSHCI AEVENDEMPA DLPSLAADFV ESKDVCKNYA EAKDVFLGMF LYEYARRHPD YSVVLLLRLA KTYETTLEKC CAAADPHECY AKVFDEFKPL VEEPQNLIKQ NCELFEQLGE YKFQNALLVR YTKKVPQMSA PTLVEVSRNL GKVGSKCCKH PEAKRMPCAE DYLSVVLNQL CVLHEKTPVS DRVTKCCTES LVNRRPCFSA LEVDETYVPK EFNAGTFTFH ADICTLSEKE RQIKKQTALV ELVKHKPKAT KEQLKAAMDD FAAFVEKCCK ADDKETCFAE EGKKLVAASQ AALGL

Entity #1467

Name: FcRn ECD beta / Type: protein
Description: neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag
Formula weight: 12.554 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P61768
Sequence:

IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDMH HHHHH

Entity #1475

Name: FcRn ECD alpha / Type: protein / Description: neonatal Fc receptor ectodomain alpha-chain / Formula weight: 30.375 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P55899
Sequence:

AESHLSLLYH LTAVSSPAPG TPAFWVSGWL GPQQYLSYNS LRGEAEPCGA WVWENQVSWY WEKETTDLRI KEKLFLEAFK ALGGKGPYTL QGLLGCELGP DNTSVPTAKF ALNGEEFMNF DLKQGTWGGD WPEALAISQR WQQQDKAANK ELTFLLFSCP HRLREHLERG RGNLEWKEPP SMRLKARPSS PGFSVLTCSA FSFYPPELQL RFLRNGLAAG TGQGDFGPNS DGSFHASSSL TVKSGDEHHY CCIVQHAGLA QPLRVELESP AKSS

Experimental information

• Beam: Instrument name: MAX IV I911-4 / City: Lund / 国: Sweden / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.
Measurement date: May 11, 2015 / Storage temperature: 25 °C / Cell temperature: 25 °C / Exposure time: 120 sec. / Number of frames: 1 / Unit: 1/A /

	Min	Max
Q	0.0094	0.4379

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 306 /

	Min	Max
Q	0.01062	0.1906
P(R) point	3	308
R	0	146.6

Result

Type of curve: single_conc /

	Experimental	Porod
MW	130 kDa	132 kDa
Volume	-	227 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.4737	0.4742	0.001
Radius of gyration, Rg	4.2 nm	4.15 nm	0.01

	Min	Max
D	-	14.66
Guinier point	3	38