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- SASDEY6: Arabidopsis thaliana anaphase promoting complex/cyclosome Subunit... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDEY6
SampleArabidopsis thaliana anaphase promoting complex/cyclosome Subunit 4 (APC4-strep)
  • Anaphase Promoting Complex/Cyclosome Subunit 4 (protein), Apc4, Arabidopsis thaliana
Function / homology
Function and homology information


plant ovule development / anaphase-promoting complex / embryo development ending in seed dormancy / anaphase-promoting complex-dependent catabolic process / protein ubiquitination / cell division / nucleus
Similarity search - Function
Anaphase-promoting complex subunit 4 / Anaphase-promoting complex subunit 4 long domain / Anaphase-promoting complex, cyclosome, subunit 4 / Anaphase-promoting complex subunit 4, WD40 domain / Anaphase-promoting complex subunit 4 WD40 domain / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Anaphase-promoting complex subunit 4
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
Contact author
  • Steven De Gieter (VUB, Vrije Universiteit Brussel, Pleinlaan 2 1050 Brussel)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2564
Type: atomic / Symmetry: P2 / Chi-square value: 3.102
Model #2566
Type: dummy / Radius of dummy atoms: 3.90 A / Symmetry: P2 / Chi-square value: 0.788 / P-value: 0.000665

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Sample

SampleName: Arabidopsis thaliana anaphase promoting complex/cyclosome Subunit 4 (APC4-strep)
Specimen concentration: 8.10-8.10
BufferName: 10 mM Tris 150 mM NaCl 1 mM TCEP / pH: 8
Entity #1353Name: Apc4 / Type: protein / Description: Anaphase Promoting Complex/Cyclosome Subunit 4 / Formula weight: 92.09 / Num. of mol.: 2 / Source: Arabidopsis thaliana / References: UniProt: O65418
Sequence: MSEMASDEEE NIIPFQLQFD KPIPFQIKIA EWNPEKDLLA MVTEDSKILL HRFNWQRLWT ISPGKPVTSL CWRPDGKAIA VGLEDGTISL HDVENGKLLR NLKPHDVAVV CLNWEEDGQS NTDESGNFSV YEDRTSRFFP PAPRPPKMPG LVAGDSSFMD DGEDSLAELS ...Sequence:
MSEMASDEEE NIIPFQLQFD KPIPFQIKIA EWNPEKDLLA MVTEDSKILL HRFNWQRLWT ISPGKPVTSL CWRPDGKAIA VGLEDGTISL HDVENGKLLR NLKPHDVAVV CLNWEEDGQS NTDESGNFSV YEDRTSRFFP PAPRPPKMPG LVAGDSSFMD DGEDSLAELS NTSFRKFNIL CTGDRDGNIC FSIFGIFQIG KINIHELSLP VPHLDEHASC KLFNASIYKV ALSKDLCRLV VMCTGELKDC DIKPREEKIN VQDLPGLHCL AMDTSIFWKR KYELHQVAQQ ASNIEDLTEV IRASLSVMNK QWADAMKTFH EKFHSLSTLI IDNGLESSPQ EEFLSLLGGA RISPALNQFL VNSLGEVGVK RVLKSVCGTG KELQQVVLDH LQPAAEIIGF RIGELRGLSR WRARYQGIGL DEMLLNEATE NTGLLLVQVQ RFMMVLSSVV QQFSNFFNWL VRSIKYLMQE PNDQLLSYNS ELLVVFLKFL YDQDPVKDLL ELSEAGDDIE IDLKTIGRVK ELLQFGGFSE CDFLQRTLAK EFQHMESSFK MAFQMPFTTI SRKISCMKLL PLCPLQLSTT QTPTTIPMSL SFYKNELSDD TPCQSGYTDY ISFQVPDETF PEISNCIGIA KGYKQNSNNE KNGYTSLEAV LLSVPNGYTC VDLSLYKDKE LVLLLNKTNT DSEGSGEACM MVVQTGDLAF ISISGSSSLN QWELEDLKGS IVNLEMENEK VRKVPHSVIA PLAVSASRGV ACVFAERRRA LVYILEEDED EEISDEKDYD IPTTENLYFQ GSAWSHPQFE KGGGSGGGSG GGSWSHPQFE K

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / : France / Type of source: X-ray synchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Arabidopsis thaliana anaphase promoting complex/cyclosome Subunit 4 (APC4-strep)
Measurement date: Mar 6, 2017 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 69 / Unit: 1/nm /
MinMax
Q0.0356 3.5101
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 716 /
MinMax
Q0.120876 3.51013
P(R) point1 716
R0 16.2
Result
Type of curve: sec /
ExperimentalPorod
MW192 kDa162 kDa
Volume-259 nm3

P(R)GuinierGuinier error
Forward scattering, I050.1 49.9 0.042
Radius of gyration, Rg4.702 nm4.64 nm0.01

MinMax
D-16.2
Guinier point19 53

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