SASDEV4: ACA8 protein (apo) in stealth nanodisc (Membrane scaffold protein...

Basic information

Entry

Database: SASBDB / ID: SASDEV4

Sample

ACA8 protein (apo) in stealth nanodisc

• Membrane scaffold protein 1D1 (deuterated, 75%) (protein), d75-msp1d1
• 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) (other), d(78,92)-POPC, Escherichia coli. (AL95 strain)
• Calcium-transporting ATPase 8, plasma membrane-type (protein), ACA8, Arabidopsis thaliana

Function / homology

Function:

response to nematode / P-type Ca2+ transporter / plasmodesma / P-type calcium transporter activity / plastid / : / calmodulin binding / ATP hydrolysis activity / ATP binding / metal ion binding / plasma membrane

Domain/homology:

Calcium-transporting P-type ATPase, N-terminal autoinhibitory domain / Ca2+-ATPase N terminal autoinhibitory domain / P-type ATPase, subfamily IIB / Cation-transporting P-type ATPase, C-terminal / Cation transporting ATPase, C-terminus / Cation transporter/ATPase, N-terminus / Cation-transporting P-type ATPase, N-terminal / Cation transporter/ATPase, N-terminus / Cation transport ATPase (P-type) / E1-E2 ATPase / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily

Component:

Calcium-transporting ATPase 8, plasma membrane-type

• Biological species: Escherichia coli. (AL95 strain); Arabidopsis thaliana (thale cress)
• Citation: Date: 2018 Dec; Title: Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin; Authors: Nitsche J / Josts I / Heidemann J / Mertens H / Maric S / Moulin M / Haertlein M / Busch S / Forsyth V / Svergun D / Uetrecht C

Contact author

• Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

Sample

• Sample: Name: ACA8 protein (apo) in stealth nanodisc / Contrast: 1.808 / Specific vol: 0.816 / Specimen concentration: 1.00-8.00 / Concentration method: A280 / Entity id: 1017 / 1018 / 1312
• Buffer: Name: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2 / pH: 7.5

Entity #1017

Name: d75-msp1d1 / Type: protein
Description: Membrane scaffold protein 1D1 (deuterated, 75%)
Formula weight: 24.661 / Num. of mol.: 2
Sequence:

GHHHHHHHDY DIPTTENLYF QGSTFSKLRE QLGPVTQEFW DNLEKETEGL RQEMSKDLEE VKAKVQPYLD DFQKKWQEEM ELYRQKVEPL RAELQEGARQ KLHELQEKLS PLGEEMRDRA RAHVDALRTH LAPYSDELRQ RLAARLEALK ENGGARLAEY HAKATEHLST LSEKAKPALE DLRQGLLPVL ESFKVSFLSA LEEYTKKLNT Q

Entity #1018

Name: d(78,92)-POPC / Type: other
Description: 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)
Formula weight: 0.76 / Source: Escherichia coli. (AL95 strain)
Sequence:

C42H82NO8P

Entity #1312

Name: ACA8 / Type: protein
Description: Calcium-transporting ATPase 8, plasma membrane-type
Formula weight: 118.209 / Num. of mol.: 1 / Source: Arabidopsis thaliana / References: UniProt: Q9LF79
Sequence:

HHHHHHHHEN LYFQGSMTSL LKSSPGRRRG GDVESGKSEH ADSDSDTFYI PSKNASIERL QQWRKAALVL NASRRFRYTL DLKKEQETRE MRQKIRSHAH ALLAANRFMD MGRESGVEKT TGPATPAGDF GITPEQLVIM SKDHNSGALE QYGGTQGLAN LLKTNPEKGI SGDDDDLLKR KTIYGSNTYP RKKGKGFLRF LWDACHDLTL IILMVAAVAS LALGIKTEGI KEGWYDGGSI AFAVILVIVV TAVSDYKQSL QFQNLNDEKR NIHLEVLRGG RRVEISIYDI VVGDVIPLNI GNQVPADGVL ISGHSLALDE SSMTGESKIV NKDANKDPFL MSGCKVADGN GSMLVTGVGV NTEWGLLMAS ISEDNGEETP LQVRLNGVAT FIGSIGLAVA AAVLVILLTR YFTGHTKDNN GGPQFVKGKT KVGHVIDDVV KVLTVAVTIV VVAVPEGLPL AVTLTLAYSM RKMMADKALV RRLSACETMG SATTICSDKT GTLTLNQMTV VESYAGGKKT DTEQLPATIT SLVVEGISQN TTGSIFVPEG GGDLEYSGSP TEKAILGWGV KLGMNFETAR SQSSILHAFP FNSEKKRGGV AVKTADGEVH VHWKGASEIV LASCRSYIDE DGNVAPMTDD KASFFKNGIN DMAGRTLRCV ALAFRTYEAE KVPTGEELSK WVLPEDDLIL LAIVGIKDPC RPGVKDSVVL CQNAGVKVRM VTGDNVQTAR AIALECGILS SDADLSEPTL IEGKSFREMT DAERDKISDK ISVMGRSSPN DKLLLVQSLR RQGHVVAVTG DGTNDAPALH EADIGLAMGI AGTEVAKESS DIIILDDNFA SVVKVVRWGR SVYANIQKFI QFQLTVNVAA LVINVVAAIS SGDVPLTAVQ LLWVNLIMDT LGALALATEP PTDHLMGRPP VGRKEPLITN IMWRNLLIQA IYQVSVLLTL NFRGISILGL EHEVHEHATR VKNTIIFNAF VLCQAFNEFN ARKPDEKNIF KGVIKNRLFM GIIVITLVLQ VIIVEFLGKF ASTTKLNWKQ WLICVGIGVI SWPLALVGKF IPVPAAPISN KLKVLKFWGK KKNSSGEGSL

Experimental information

• Beam: Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.3 mm
• Detector: Name: Pilatus 2M

Scan

Title: ACA8 protein (apo) in stealth nanodisc / Measurement date: Sep 8, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.0249	5.064

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1069 /

	Min	Max
Q	0.110477	3.05776
P(R) point	1	1069
R	0	20

Result

Type of curve: single_conc
Comments: 'ACA8 protein (apo) encapsulated in a partially deuterated

	Experimental	Experimental error	Porod	Porod error
MW	259 kDa	25	391 kDa	40
Volume	-	-	626 nm3	50

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.093	0.001	0.094	0.001
Radius of gyration, Rg	5.38 nm	0.01	5.3 nm	0.01

	Min	Max	Error
D	-	20	0.5
Guinier point	32	78	-