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- SASDEV4: ACA8 protein (apo) in stealth nanodisc (Membrane scaffold protein... -

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Basic information

Entry
Database: SASBDB / ID: SASDEV4
SampleACA8 protein (apo) in stealth nanodisc
  • Membrane scaffold protein 1D1 (deuterated, 75%) (protein), d75-msp1d1
  • 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) (other), d(78,92)-POPC, Escherichia coli. (AL95 strain)
  • Calcium-transporting ATPase 8, plasma membrane-type (protein), ACA8, Arabidopsis thaliana
Function / homology
Function and homology information


P-type Ca2+ transporter / P-type calcium transporter activity / plasmodesma / response to nematode / plastid / calmodulin binding / ATP hydrolysis activity / ATP binding / metal ion binding / plasma membrane
Similarity search - Function
Calcium-transporting P-type ATPase, N-terminal autoinhibitory domain / Ca2+-ATPase N terminal autoinhibitory domain / P-type ATPase, subfamily IIB / Cation-transporting P-type ATPase, C-terminal / Cation transporting ATPase, C-terminus / Cation transporter/ATPase, N-terminus / Cation-transporting P-type ATPase, N-terminal / Cation transporter/ATPase, N-terminus / P-type ATPase, cytoplasmic domain N / P-type ATPase actuator domain ...Calcium-transporting P-type ATPase, N-terminal autoinhibitory domain / Ca2+-ATPase N terminal autoinhibitory domain / P-type ATPase, subfamily IIB / Cation-transporting P-type ATPase, C-terminal / Cation transporting ATPase, C-terminus / Cation transporter/ATPase, N-terminus / Cation-transporting P-type ATPase, N-terminal / Cation transporter/ATPase, N-terminus / P-type ATPase, cytoplasmic domain N / P-type ATPase actuator domain / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily
Similarity search - Domain/homology
Calcium-transporting ATPase 8, plasma membrane-type
Similarity search - Component
Biological speciesEscherichia coli. (AL95 strain)
Arabidopsis thaliana (thale cress)
CitationDate: 2018 Dec
Title: Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin
Authors: Nitsche J / Josts I / Heidemann J / Mertens H / Maric S / Moulin M / Haertlein M / Busch S / Forsyth V / Svergun D / Uetrecht C
Contact author
  • Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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Models

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Sample

SampleName: ACA8 protein (apo) in stealth nanodisc / Contrast: 1.808 / Specific vol: 0.816 / Specimen concentration: 1.00-8.00 / Concentration method: A280 / Entity id: 1017 / 1018 / 1312
BufferName: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2 / pH: 7.5
Entity #1017Name: d75-msp1d1 / Type: protein
Description: Membrane scaffold protein 1D1 (deuterated, 75%)
Formula weight: 24.661 / Num. of mol.: 2
Sequence: GHHHHHHHDY DIPTTENLYF QGSTFSKLRE QLGPVTQEFW DNLEKETEGL RQEMSKDLEE VKAKVQPYLD DFQKKWQEEM ELYRQKVEPL RAELQEGARQ KLHELQEKLS PLGEEMRDRA RAHVDALRTH LAPYSDELRQ RLAARLEALK ENGGARLAEY HAKATEHLST ...Sequence:
GHHHHHHHDY DIPTTENLYF QGSTFSKLRE QLGPVTQEFW DNLEKETEGL RQEMSKDLEE VKAKVQPYLD DFQKKWQEEM ELYRQKVEPL RAELQEGARQ KLHELQEKLS PLGEEMRDRA RAHVDALRTH LAPYSDELRQ RLAARLEALK ENGGARLAEY HAKATEHLST LSEKAKPALE DLRQGLLPVL ESFKVSFLSA LEEYTKKLNT Q
Entity #1018Name: d(78,92)-POPC / Type: other
Description: 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)
Formula weight: 0.76 / Source: Escherichia coli. (AL95 strain)
Sequence:
C42H82NO8P
Entity #1312Name: ACA8 / Type: protein
Description: Calcium-transporting ATPase 8, plasma membrane-type
Formula weight: 118.209 / Num. of mol.: 1 / Source: Arabidopsis thaliana / References: UniProt: Q9LF79
Sequence: HHHHHHHHEN LYFQGSMTSL LKSSPGRRRG GDVESGKSEH ADSDSDTFYI PSKNASIERL QQWRKAALVL NASRRFRYTL DLKKEQETRE MRQKIRSHAH ALLAANRFMD MGRESGVEKT TGPATPAGDF GITPEQLVIM SKDHNSGALE QYGGTQGLAN LLKTNPEKGI ...Sequence:
HHHHHHHHEN LYFQGSMTSL LKSSPGRRRG GDVESGKSEH ADSDSDTFYI PSKNASIERL QQWRKAALVL NASRRFRYTL DLKKEQETRE MRQKIRSHAH ALLAANRFMD MGRESGVEKT TGPATPAGDF GITPEQLVIM SKDHNSGALE QYGGTQGLAN LLKTNPEKGI SGDDDDLLKR KTIYGSNTYP RKKGKGFLRF LWDACHDLTL IILMVAAVAS LALGIKTEGI KEGWYDGGSI AFAVILVIVV TAVSDYKQSL QFQNLNDEKR NIHLEVLRGG RRVEISIYDI VVGDVIPLNI GNQVPADGVL ISGHSLALDE SSMTGESKIV NKDANKDPFL MSGCKVADGN GSMLVTGVGV NTEWGLLMAS ISEDNGEETP LQVRLNGVAT FIGSIGLAVA AAVLVILLTR YFTGHTKDNN GGPQFVKGKT KVGHVIDDVV KVLTVAVTIV VVAVPEGLPL AVTLTLAYSM RKMMADKALV RRLSACETMG SATTICSDKT GTLTLNQMTV VESYAGGKKT DTEQLPATIT SLVVEGISQN TTGSIFVPEG GGDLEYSGSP TEKAILGWGV KLGMNFETAR SQSSILHAFP FNSEKKRGGV AVKTADGEVH VHWKGASEIV LASCRSYIDE DGNVAPMTDD KASFFKNGIN DMAGRTLRCV ALAFRTYEAE KVPTGEELSK WVLPEDDLIL LAIVGIKDPC RPGVKDSVVL CQNAGVKVRM VTGDNVQTAR AIALECGILS SDADLSEPTL IEGKSFREMT DAERDKISDK ISVMGRSSPN DKLLLVQSLR RQGHVVAVTG DGTNDAPALH EADIGLAMGI AGTEVAKESS DIIILDDNFA SVVKVVRWGR SVYANIQKFI QFQLTVNVAA LVINVVAAIS SGDVPLTAVQ LLWVNLIMDT LGALALATEP PTDHLMGRPP VGRKEPLITN IMWRNLLIQA IYQVSVLLTL NFRGISILGL EHEVHEHATR VKNTIIFNAF VLCQAFNEFN ARKPDEKNIF KGVIKNRLFM GIIVITLVLQ VIIVEFLGKF ASTTKLNWKQ WLICVGIGVI SWPLALVGKF IPVPAAPISN KLKVLKFWGK KKNSSGEGSL

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.3 mm
DetectorName: Pilatus 2M
Scan
Title: ACA8 protein (apo) in stealth nanodisc / Measurement date: Sep 8, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0249 5.064
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1069 /
MinMax
Q0.110477 3.05776
P(R) point1 1069
R0 20
Result
Type of curve: single_conc
Comments: 'ACA8 protein (apo) encapsulated in a partially deuterated
ExperimentalExperimental errorPorodPorod error
MW259 kDa25 391 kDa40
Volume--626 nm350

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.093 0.001 0.094 0.001
Radius of gyration, Rg5.38 nm0.01 5.3 nm0.01

MinMaxError
D-20 0.5
Guinier point32 78 -

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