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- SASDEP3: Senescence-associated E3 ubiquitin ligase 1, 7.3 mg/ml -

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Basic information

Entry
Database: SASBDB / ID: SASDEP3
SampleSenescence-associated E3 ubiquitin ligase 1, 7.3 mg/ml
  • Senescence-associated E3 ubiquitin ligase 1 (protein), SAUL1, Arabidopsis thaliana
Function / homology
Function and homology information


regulation of abscisic acid biosynthetic process / regulation of chlorophyll catabolic process / negative regulation of abscisic acid biosynthetic process / transmembrane receptor protein serine/threonine kinase binding / regulation of chlorophyll biosynthetic process / leaf senescence / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / protein ubiquitination / nucleus ...regulation of abscisic acid biosynthetic process / regulation of chlorophyll catabolic process / negative regulation of abscisic acid biosynthetic process / transmembrane receptor protein serine/threonine kinase binding / regulation of chlorophyll biosynthetic process / leaf senescence / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / protein ubiquitination / nucleus / plasma membrane / cytoplasm
Similarity search - Function
: / PUB protein, U-box domain, plant / U-box domain / U-box domain profile. / Modified RING finger domain / U-box domain / Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeat / Armadillo/beta-catenin-like repeats / Armadillo ...: / PUB protein, U-box domain, plant / U-box domain / U-box domain profile. / Modified RING finger domain / U-box domain / Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeat / Armadillo/beta-catenin-like repeats / Armadillo / Armadillo-like helical / Armadillo-type fold / Zinc finger, RING/FYVE/PHD-type
Similarity search - Domain/homology
U-box domain-containing protein 44
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
Contact author
  • Al Kikhney (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1242
Type: atomic / Radius of dummy atoms: 1.90 A / Symmetry: P222 / Chi-square value: 1.21
Model #1287
Type: dummy / Software: (r9217) / Radius of dummy atoms: 5.00 A / Symmetry: P222 / Chi-square value: 1.032 / P-value: 0.725552

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Sample

SampleName: Senescence-associated E3 ubiquitin ligase 1, 7.3 mg/ml
Specimen concentration: 7.3 mg/ml
BufferName: 50 mM Tris, 250 mM NaCl / pH: 9
Entity #670Name: SAUL1 / Type: protein / Description: Senescence-associated E3 ubiquitin ligase 1 / Formula weight: 88.79 / Num. of mol.: 4 / Source: Arabidopsis thaliana / References: UniProt: Q9LM76
Sequence: GPLGSMVGSS DGDQSDDSSH FERGVDHIYE AFICPLTKEV MHDPVTLENG RTFEREAIEK WFKECRDSGR PPSCPLTSQE LTSTDVSASI ALRNTIEEWR SRNDAAKLDI ARQSLFLGNA ETDILQALMH VRQICRTIRS NRHGVRNSQL IHMIIDMLKS TSHRVRYKAL ...Sequence:
GPLGSMVGSS DGDQSDDSSH FERGVDHIYE AFICPLTKEV MHDPVTLENG RTFEREAIEK WFKECRDSGR PPSCPLTSQE LTSTDVSASI ALRNTIEEWR SRNDAAKLDI ARQSLFLGNA ETDILQALMH VRQICRTIRS NRHGVRNSQL IHMIIDMLKS TSHRVRYKAL QTLQVVVEGD DESKAIVAEG DTVRTLVKFL SHEPSKGREA AVSLLFELSK SEALCEKIGS IHGALILLVG LTSSNSENVS IVEKADRTLE NMERSEEIVR QMASYGRLQP LLGKLLEGSP ETKLSMASFL GELPLNNDVK VLVAQTVGSS LVDLMRSGDM PQREAALKAL NKISSFEGSA KVLISKGILP PLIKDLFYVG PNNLPIRLKE VSATILANIV NIGYDFDKAT LVSENRVENL LHLISNTGPA IQCKLLEVLV GLTSCPKTVP KVVYAIKTSG AIISLVQFIE VRENDDLRLA SIKLLHNLSP FMSEELAKAL CGTAGQLGSL VAIISEKTPI TEEQAAAAGL LAELPDRDLG LTQEMLEVGA FEKIISKVFG IRQGDIKGMR FVNPFLEGLV RILARITFVF NKEARAINFC REHDVASLFL HLLQSNGQDN IQMVSAMALE NLSLESIKLT RMPDPPPVNY CGSIFSCVRK PHVVNGLCKI HQGICSLRET FCLVEGGAVE KLVALLDHEN VKVVEAALAA LSSLLEDGLD VEKGVKILDE ADGIRHILNV LRENRTERLT RRAVWMVERI LRIEDIAREV AEEQSLSAAL VDAFQNADFR TRQIAENALK HIDKIPNFSS IFPNIA

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.123987 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 2M
Scan
Title: Senescence-associated E3 ubiquitin ligase 1, 7.3 mg/ml
Measurement date: Jun 2, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.027 4.8012
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 510 /
MinMax
Q0.0612974 1.40612
P(R) point1 510
R0 24
Result
Type of curve: single_conc
Comments: Monomer:dimer:tetramer fractions by volume: 5:3:92.
ExperimentalPorod
MW310 kDa310 kDa

GuinierP(R)
Forward scattering, I010367.8 -
Radius of gyration, Rg6.54 nm6.558 nm

MinMax
D-24
Guinier point14 54

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