SASDED2: Polyglutamine tract-binding protein 1 (PQBP-1) (Polyglutamine-bin...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDED2

試料

Polyglutamine tract-binding protein 1 (PQBP-1)

• Polyglutamine-binding protein 1 (protein), PQBP-1, Homo sapiens

機能・相同性

分子機能:

neuronal ribonucleoprotein granule / alternative mRNA splicing, via spliceosome / regulation of dendrite morphogenesis / cellular response to exogenous dsRNA / regulation of RNA splicing / positive regulation of type I interferon production / ribonucleoprotein complex binding / positive regulation of defense response to virus by host / activation of innate immune response / mRNA Splicing - Major Pathway / cytoplasmic stress granule / neuron projection development / double-stranded DNA binding / defense response to virus / transcription coactivator activity / nuclear body / nuclear speck / innate immune response / regulation of DNA-templated transcription / DNA binding / nucleoplasm / nucleus / cytoplasm

ドメイン・相同性:

WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain

構成要素:

Polyglutamine-binding protein 1

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: Biophys J / 年: 2012; タイトル: Solution model of the intrinsically disordered polyglutamine tract-binding protein-1.; 著者: Martin Rees / Christian Gorba / Cesira de Chiara / Tam T T Bui / Mitla Garcia-Maya / Alex F Drake / Hitoshi Okazawa / Annalisa Pastore / Dmitri Svergun / Yu Wai Chen / ; 要旨: Polyglutamine tract-binding protein-1 (PQBP-1) is a 265-residue nuclear protein that is involved in transcriptional regulation. In addition to its role in the molecular pathology of the polyglutamine expansion diseases, mutations of the protein are associated with X-linked mental retardation. PQBP-1 binds specifically to glutamine repeat sequences and proline-rich regions, and interacts with RNA polymerase II and the spliceosomal protein U5-15kD. In this work, we obtained a biophysical characterization of this protein by employing complementary structural methods. PQBP-1 is shown to be a moderately compact but largely disordered molecule with an elongated shape, having a Stokes radius of 3.7 nm and a maximum molecular dimension of 13 nm. The protein is monomeric in solution, has residual β-structure, and is in a premolten globule state that is unaffected by natural osmolytes. Using small-angle x-ray scattering data, we were able to generate a low-resolution, three-dimensional model of PQBP-1.

登録者

• Yu Wai Chen (King's College London)

モデル

• モデル #2194: ; タイプ: dummy / ソフトウェア: (1.1.0 r980) / ダミー原子の半径: 2.70 A / カイ2乗値: 1.018081 / P-value: 0.058031; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Polyglutamine tract-binding protein 1 (PQBP-1) / 試料濃度: 7.00-13.00
• バッファ: 名称: 20 mM Tris, 150 mM NaCl, 1mM DTT, / pH: 7

要素 #1201

名称: PQBP-1 / タイプ: protein / 記述: Polyglutamine-binding protein 1 / 分子量: 30.626 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: O60828
配列:

GPMPLPVALQ TRLAKRGILK HLEPEPEEEI IAEDYDDDPV DYEATRLEGL PPSWYKVFDP SCGLPYYWNA DTDLVSWLSP HDPNSVVTKS AKKLRSSNAD AEEKLDRSHD KSDRGHDKSD RSHEKLDRGH DKSDRGHDKS DRDRERGYDK VDRERERDRE RDRDRGYDKA DREEGKERRH HRREELAPYP KSKKAVSRKD EELDPMDPSS YSDAPRGTWS TGLPKRNEAK TGADTTAAGP LFQQRPYPSP GAVLRANAEA SRTKQQD

実験情報

• ビーム: 設備名称: DORIS III EMBL X33 / 地域: Hamburg / 国: Germany / 形状: 0.6 / 線源: X-ray synchrotron / 波長: 0.15 Å / スペクトロメータ・検出器間距離: 2.7 mm
• 検出器: 名称: Pilatus 1M-W / Pixsize x: 0.172 mm

スキャン

タイトル: Polyglutamine tract-binding protein 1 (PQBP-1)
測定日: 2009年11月18日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 15 sec. / フレーム数: 8 / 単位: 1/nm /

	Min	Max
Q	0.0775	6.2077

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 500 /

	Min	Max
Q	0.07879	2.741
P(R) point	1	500
R	0	13

結果

カーブのタイプ: merged

	実験値	Standard	Standard error	Porod
分子量	30.6 kDa	30 kDa	3	35 kDa
体積	-	-	-	51 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	40.75	40.49	-
慣性半径, Rg	3.82 nm	3.72 nm	0.2

	Min	Max
D	-	13
Guinier point	30	97