SASDEC2: Cell wall synthesis protein Wag31: Polymer tbWag31 T73A

基本情報

登録情報

データベース: SASBDB / ID: SASDEC2

試料

Cell wall synthesis protein Wag31: Polymer tbWag31 T73A

• Cell wall synthesis protein Wag31 (protein), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

機能・相同性

分子機能:

peptidoglycan-based cell wall biogenesis / regulation of growth rate / cell pole / peptidoglycan-based cell wall / regulation of cell shape / protein stabilization / 細胞周期 / 細胞分裂 / 細胞膜 / 細胞質

ドメイン・相同性:

DivIVA family / DivIVA domain / DivIVA protein

構成要素:

Cell wall synthesis protein Wag31

• 生物種: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (結核菌)
• 引用: 日付: 2018 Aug 18; タイトル: Higher order assembling of the mycobacterial essential polar growth factor DivIVA/Wag31; 著者: Choukate K / Gupta A / Basu B / Virk K / Ganguli M

登録者

• Komal Choukate (CSIR, CSIR-Institute of Microbial Technology, Chandigarh, India)

モデル

試料

• 試料: 名称: Cell wall synthesis protein Wag31: Polymer tbWag31 T73A; 試料濃度: 2.7 mg/ml
• バッファ: 名称: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME); pH: 7.5

要素 #1213

タイプ: protein / 記述: Cell wall synthesis protein Wag31 / 分子量: 29.922
由来: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
参照: UniProt: P9WMU1
配列:

MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAAQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ADAMLADAQS RSEAQLRQAQ EKADALQADA ERKHSEIMGT INQQRAVLEG RLEQLRTFER EYRTRLKTYL ESQLEELGQR GSAAPVDSNA DAGGFDQFNR GKN

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotronシンクロトロン / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 2.87 mm
• 検出器: 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm

スキャン

タイトル: Cell wall synthesis protein Wag31:Polymer tbWag31 T73A
測定日: 2017年12月15日 / 保管温度: 4 °C / セル温度: 4 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0385	4.9335

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 367 /

	Min	Max
Q	0.08783	3.524
P(R) point	11	377
R	0	13.55

結果

Experimental MW: 29.9 kDa / カーブのタイプ: single_conc
コメント: The cell wall synthesis protein Wag31: Polymer tbWag31 T73A forms a large super-macromolecular assembly (supported by atomic force microscopy, AFM) and as a result there is a very limited angular range to estimate the radius of gyration, Rg, using the Guinier approximation. In addition, the quoted monomer molecular weight does not apply to this sample. The probable distribution of real-space scattering pair vector lengths (p(r) vs r profile or PDDF) represents the cross-sectional pair distribution function for rod-like particle (option 4 in GNOM) and the subsequent data validation tools for this entry are based on the corresponding cross section parameters (Rg of cross section and Dmax of cross section). The I(s) is recorded in a relative scale in kDa units assuming a 1 mg/ml sample concentration. However the experimental concentration was found to be 2.7 mg/ml (assessed using a Bradford assay).

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	34.66	0.08	990.33	17.9
慣性半径, Rg	3.32 nm	0.021	21.8 nm	0.409

	Min	Max
D	-	13.55
Guinier point	1	6