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- SASDEC2: Cell wall synthesis protein Wag31: Polymer tbWag31 T73A -

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Basic information

Entry
Database: SASBDB / ID: SASDEC2
SampleCell wall synthesis protein Wag31: Polymer tbWag31 T73A
  • Cell wall synthesis protein Wag31 (protein), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function / homology
Function and homology information


peptidoglycan-based cell wall biogenesis / regulation of growth rate / cell pole / peptidoglycan-based cell wall / regulation of cell shape / protein stabilization / cell cycle / cell division / plasma membrane / cytoplasm
Similarity search - Function
DivIVA family / DivIVA domain / DivIVA protein
Similarity search - Domain/homology
Cell wall synthesis protein Wag31
Similarity search - Component
Biological speciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
CitationDate: 2018 Aug 18
Title: Higher order assembling of the mycobacterial essential polar growth factor DivIVA/Wag31
Authors: Choukate K / Gupta A / Basu B / Virk K / Ganguli M
Contact author
  • Komal Choukate (CSIR, CSIR-Institute of Microbial Technology, Chandigarh, India)

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Structure visualization

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Models

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Sample

SampleName: Cell wall synthesis protein Wag31: Polymer tbWag31 T73A
Specimen concentration: 2.7 mg/ml
BufferName: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)
pH: 7.5
Entity #1213Type: protein / Description: Cell wall synthesis protein Wag31 / Formula weight: 29.922
Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
References: UniProt: P9WMU1
Sequence: MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAAQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ...Sequence:
MHHHHHHENL YFQGPLTPAD VHNVAFSKPP IGKRGYNEDE VDAFLDLVEN ELTRLIEENS DLRQRINELD QELAAGGGAG VTPQAAQAIP AYEPEPGKPA PAAVSAGMNE EQALKAARVL SLAQDTADRL TNTAKAESDK MLADARANAE QILGEARHTA DATVAEARQR ADAMLADAQS RSEAQLRQAQ EKADALQADA ERKHSEIMGT INQQRAVLEG RLEQLRTFER EYRTRLKTYL ESQLEELGQR GSAAPVDSNA DAGGFDQFNR GKN

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 2.87 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Cell wall synthesis protein Wag31:Polymer tbWag31 T73A
Measurement date: Dec 15, 2017 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0385 4.9335
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 367 /
MinMax
Q0.08783 3.524
P(R) point11 377
R0 13.55
Result
Experimental MW: 29.9 kDa / Type of curve: single_conc
Comments: The cell wall synthesis protein Wag31: Polymer tbWag31 T73A forms a large super-macromolecular assembly (supported by atomic force microscopy, AFM) and as a result there is a very limited ...Comments: The cell wall synthesis protein Wag31: Polymer tbWag31 T73A forms a large super-macromolecular assembly (supported by atomic force microscopy, AFM) and as a result there is a very limited angular range to estimate the radius of gyration, Rg, using the Guinier approximation. In addition, the quoted monomer molecular weight does not apply to this sample. The probable distribution of real-space scattering pair vector lengths (p(r) vs r profile or PDDF) represents the cross-sectional pair distribution function for rod-like particle (option 4 in GNOM) and the subsequent data validation tools for this entry are based on the corresponding cross section parameters (Rg of cross section and Dmax of cross section). The I(s) is recorded in a relative scale in kDa units assuming a 1 mg/ml sample concentration. However the experimental concentration was found to be 2.7 mg/ml (assessed using a Bradford assay).
P(R)P(R) errorGuinierGuinier error
Forward scattering, I034.66 0.08 990.33 17.9
Radius of gyration, Rg3.32 nm0.021 21.8 nm0.409

MinMax
D-13.55
Guinier point1 6

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