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- SASDEA9: Calredoxin with Calcium (Calredoxin, Redox protein from Chlamydom... -

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Basic information

Entry
Database: SASBDB / ID: SASDEA9
SampleCalredoxin with Calcium
  • Calredoxin, Redox protein from Chlamydomonas reinhardtii (protein), CRX, Chlamydomonas reinhardtii
Function / homology
Function and homology information


calcium ion binding
Similarity search - Function
Thioredoxin / Thioredoxin domain profile. / Thioredoxin domain / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Thioredoxin-like superfamily
Similarity search - Domain/homology
Thioredoxin domain-containing protein
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Contact author
  • Ratana Charoenwattanasatien (Synchrotron Light Research Institue)

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Structure visualization

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Models

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Sample

SampleName: Calredoxin with Calcium / Specimen concentration: 5 mg/ml
BufferName: 20 mM Tris, 150 mM NaCl, 1 mM DTT, 5 mM Ca / pH: 8
Entity #1414Name: CRX / Type: protein
Description: Calredoxin, Redox protein from Chlamydomonas reinhardtii
Formula weight: 39.901 / Num. of mol.: 1 / Source: Chlamydomonas reinhardtii / References: UniProt: A0A2K3DZB3
Sequence: MCSARSVRVV PRAATFEGLM DDASKAKMEE LERRFKMADV DGNGHIDREE LRNLLESMES GEVYMMSQHW LPEDELERCM EQYDVNKDGV ISFEEFKQII YDGLLLEGTL AEYESAFKAV DKSGNGTIGA TELSKLFASL GNPVSLEKLV DLMQMYDKDD SGQIEFPEFL ...Sequence:
MCSARSVRVV PRAATFEGLM DDASKAKMEE LERRFKMADV DGNGHIDREE LRNLLESMES GEVYMMSQHW LPEDELERCM EQYDVNKDGV ISFEEFKQII YDGLLLEGTL AEYESAFKAV DKSGNGTIGA TELSKLFASL GNPVSLEKLV DLMQMYDKDD SGQIEFPEFL LMFRNSLLDL KDMTTYMTLD EAGAGSSGSL VDAVEGDMTL IFSEEELDAL ISANPDKLVV VFGALTWCRP CKGMQRPVQK LAEHYKDHIV FVKLFGNANK QTKRIFKERF QIRSTPCFIT LRKGEPVYTQ TGSNKEKLEA GLRSLIANPP VGMIYPSAEA LAALQDPNSS SVDKLAAALE HHHHHH

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Experimental information

BeamInstrument name: Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University Rigaku BioSAXS-1000
City: Yokohama / : Japan / Type of source: X-ray in house / Wavelength: 0.15418 Å
DetectorName: Pilatus 100K / Pixsize x: 172 mm
Scan
Title: Calredoxin with Calcium / Measurement date: Nov 17, 2015 / Cell temperature: 20 °C / Exposure time: 120 sec. / Number of frames: 2 / Unit: 1/A /
MinMax
Q0.0033 0.6681
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 319 /
MinMax
Q0.0135901 0.230859
P(R) point1 319
R0 115.6
Result
Type of curve: single_conc /
ExperimentalPorod
MW42.05 kDa42 kDa
Volume-68 nm3

P(R)GuinierGuinier error
Forward scattering, I00.7076 0.7 0.003
Radius of gyration, Rg3.19 nm3.06 nm0.08

MinMax
D-11.56
Guinier point16 58

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