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- SASDEA8: Full length of DnaG (DNA Primase) from Bacillus subtilis -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDEA8
SampleFull length of DnaG (DNA Primase) from Bacillus subtilis
  • DNA primase (protein), Bacillus subtilis (strain 168)
Function / homology
Function and homology information


DNA primase DnaG / primosome complex / : / DNA replication, synthesis of primer / DNA helicase activity / DNA-directed RNA polymerase complex / DNA binding / zinc ion binding / ATP binding / cytoplasm
Similarity search - Function
DNA primase, DnaB-helicase binding domain / DnaB-helicase binding domain of primase / Zinc finger, CHC2-type / DNA primase, DnaG / DNA primase, catalytic core, N-terminal / DNA primase DnaG, bacteria / Bacterial DnaG primase, TOPRIM domain / DNA primase, catalytic core, N-terminal domain superfamily / : / CHC2 zinc finger ...DNA primase, DnaB-helicase binding domain / DnaB-helicase binding domain of primase / Zinc finger, CHC2-type / DNA primase, DnaG / DNA primase, catalytic core, N-terminal / DNA primase DnaG, bacteria / Bacterial DnaG primase, TOPRIM domain / DNA primase, catalytic core, N-terminal domain superfamily / : / CHC2 zinc finger / DNA primase catalytic core, N-terminal domain / zinc finger / DNA Primase, CHC2-type zinc finger / Toprim-like / DNA helicase, DnaB-like, N-terminal domain superfamily / DNA helicase DnaB, N-terminal/DNA primase DnaG, C-terminal / TOPRIM / Toprim domain profile. / TOPRIM domain
Similarity search - Domain/homology
Biological speciesBacillus subtilis (strain 168) (bacteria)
Contact author
  • Zhongchuan Liu (Chengdu Institute of Biology, Chinese academy of Sciences, Chengdu, China)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2635
Type: mix / Radius of dummy atoms: 1.90 A / Chi-square value: 0.95 / P-value: 0.554373
Model #2638
Type: dummy / Radius of dummy atoms: 5.00 A / Chi-square value: 1.176 / P-value: 0.050515

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Sample

SampleName: Full length of DnaG (DNA Primase) from Bacillus subtilis
Specimen concentration: 1.00-7.00
BufferName: 25 mM Tris-HCl,100 mM NaCl / pH: 8 / Comment: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM DTT
Entity #1387Type: protein / Description: DNA primase / Formula weight: 68.735 / Num. of mol.: 1 / Source: Bacillus subtilis (strain 168) / References: UniProt: P05096
Sequence: MGNRIPDEIV DQVQKSADIV EVIGDYVQLK KQGRNYFGLC PFHGESTPSF SVSPDKQIFH CFGCGAGGNV FSFLRQMEGY SFAESVSHLA DKYQIDFPDD ITVHSGARPE SSGEQKMAEA HELLKKFYHH LLINTKEGQE ALDYLLSRGF TKELINEFQI GYALDSWDFI ...Sequence:
MGNRIPDEIV DQVQKSADIV EVIGDYVQLK KQGRNYFGLC PFHGESTPSF SVSPDKQIFH CFGCGAGGNV FSFLRQMEGY SFAESVSHLA DKYQIDFPDD ITVHSGARPE SSGEQKMAEA HELLKKFYHH LLINTKEGQE ALDYLLSRGF TKELINEFQI GYALDSWDFI TKFLVKRGFS EAQMEKAGLL IRREDGSGYF DRFRNRVMFP IHDHHGAVVA FSGRALGSQQ PKYMNSPETP LFHKSKLLYN FYKARLHIRK QERAVLFEGF ADVISAVSSD VKESIATMGT SLTDDHVKIL RRNVEEIILC YDSDKAGYEA TLKASELLQK KGCKVRVAMI PDGLDPDDYI KKFGGEKFKN DIIDASVTVM AFKMQYFRKG KNLSDEGDRL AYIKDVLKEI STLSGSLEQE VYVKQLASEF SLSQESLTEQ LSVFSKQNKP ADNSGETKTR RAHLTTKARQ KRLRPAYENA ERLLLAHMLR DRSVIKKVID RVGFQFNIDE HRALAAYLYA FYEEGAELTP QHLMARVTDD HISQLLSDIL MLQVNQELSE AELSDYVKKV LNQRNWSMIK EKEAERAEAE RQKDFLRAAS LAQEIVTLNR SLK

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Experimental information

BeamInstrument name: Shanghai Synchrotron Radiation Facility (SSRF) BL19U2
City: Shanghai / 国: China / Type of source: X-ray synchrotron / Wavelength: 0.154 Å / Dist. spec. to detc.: 2 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Full length of DnaG (DNA Primase) from Bacillus subtilis
Measurement date: Oct 1, 2018 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 20 / Unit: 1/A /
MinMax
Q0.0084 0.3954
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 433 /
MinMax
Q0.0112342 0.213351
P(R) point1 433
R0 145
Result
Type of curve: merged
ExperimentalStandardStandard errorPorod
MW70 kDa70 kDa2 74 kDa
Volume---119 nm3

P(R)GuinierGuinier error
Forward scattering, I090.9 89.22 0.24
Radius of gyration, Rg3.94 nm3.74 nm0.1

MinMax
D-14.5
Guinier point7 56

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