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- SASDE86: C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A -

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Basic information

Entry
Database: SASBDB / ID: SASDE86
SampleC. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A
  • ATP-citrate lyase (protein), ACL, Chlorobium limicola
Biological speciesChlorobium limicola (bacteria)
CitationDate: 2019 Apr 3
Title: Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle
Authors: Verschueren K / Blanchet C / Felix J / Dansercoer A / De Vos D / Bloch Y / Van Beeumen J / Svergun D / Gutsche I / Savvides S
Contact author
  • Clement Blanchet (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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Models

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Sample

SampleName: C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A
Specimen concentration: 16 mg/ml
BufferName: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA
pH: 7.2
Entity #1334Name: ACL / Type: protein / Description: ATP-citrate lyase / Formula weight: 107.314 / Num. of mol.: 4 / Source: Chlorobium limicola
Sequence: AKILEGPAMK LFNKWGIPVP NYVVIMDPDR LAQLGEANKW LRESKLVVKA HEAIGGRFKL GLVKIGLNLE QAIEAAKEML GAKVGTAEIG QVIIAEMLEH DAEFYVSIIG NKDGAELLIS KHGGVDIEDN WDSVRRIQIE LDENPTIEQL TELAKDAGFE GEIAERVGKI ...Sequence:
AKILEGPAMK LFNKWGIPVP NYVVIMDPDR LAQLGEANKW LRESKLVVKA HEAIGGRFKL GLVKIGLNLE QAIEAAKEML GAKVGTAEIG QVIIAEMLEH DAEFYVSIIG NKDGAELLIS KHGGVDIEDN WDSVRRIQIE LDENPTIEQL TELAKDAGFE GEIAERVGKI CSRLILCFDN EDAQSIEINP LVIRKSDMRF AALDAVMNVD YDARFRHADW DFKPVSEIGR PFTEAEQQIM EIDSRIKGSV KFVEVPGGEI ALLTAGGGAS VFYADAVVAR GGTIANYAEY SGDPADWAVE ALTETICRLP NIKHIIVGGA IANFTDVKAT FSGIINGFRE SKSKGYLEGV KIWVRRGGPN EAQGLAAIKQ LQEEGFDIHV YDRSMPMTDI VDLAMKSSIL ANKDTRAVII GGVAGVNAAK RMAQFDFLVN RPLTVQAFVY PPEAGQQKEI FRGGELKNVT VYDSLAPALE EHPDINTALI YLGASRAAQA AKEALESPNI QLVSMITEGV PEKDAKRLKK LAQKLGKMLN GPSSIGIMSA GECRLGVIGG EFKNLKLCNL YRQGSFGVLT KSGGLSNEAM WLCAQNGDGI TSAVAIGGDA YPGTDFVTYL EMFEKDPATK AVVMIGEVGG NLEEEAAEWL AAEPRRIKLI AAIGGTCQEV LKGAGSARSK MNALRDAGAY VPDTFGGLSK EIKKVYEELI AAGEISTEID EAVLPELPPR VQEVMKQGEV IVEPLIRTTI SDDRGEEPRY AGYAASELCS KGYGIEDVIG LLWNKKLPTR EESEIIKRIV MISADHGPAV SGAFGSILAA CAGIDMPQAV SAGMTMIGPR FGGAVTNAGK YFKMAVEDYP NDIPGFLSWM KKNVGPVPGI GHRVKSVKNP DQRVKYLVSY IKNETSLHTP CLDYALEVEK VTTAKKGNLI LNVDGTIGCI LMDLDFPVHS LNGFFVLART IGMIGHWIDQ NNQNSRLIRL YDYLINYAVK PEQEVPEK

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: C. limicola ATP-citrate lyase ACL in HBS + Citrate + Coenzyme A
Measurement date: Sep 4, 2017 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 900 / Unit: 1/nm /
MinMax
Q0.0249 5.064
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 489 /
MinMax
Q0.0580443 1.40474
P(R) point1 489
R0 17.5
Result
Type of curve: sec /
ExperimentalPorod
MW420 kDa494 kDa
Volume-791 nm3

P(R)GuinierGuinier error
Forward scattering, I0111400 110407 61
Radius of gyration, Rg5.8 nm5.69 nm0.03

MinMax
D-17.5
Guinier point13 54

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