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- SASDDY9: Protein translocase subunit SecA (full length, amino acids 1-901) -

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Basic information

Entry
Database: SASBDB / ID: SASDDY9
SampleProtein translocase subunit SecA (full length, amino acids 1-901)
  • Protein translocase subunit SecA (protein), Escherichia coli (strain K12)
Function / homology
Function and homology information


preprotein binding / protein-exporting ATPase activity / cell envelope Sec protein transport complex / protein-secreting ATPase / protein transport by the Sec complex / intracellular protein transmembrane transport / protein import / protein targeting to membrane / protein secretion / ribonucleoprotein complex binding ...preprotein binding / protein-exporting ATPase activity / cell envelope Sec protein transport complex / protein-secreting ATPase / protein transport by the Sec complex / intracellular protein transmembrane transport / protein import / protein targeting to membrane / protein secretion / ribonucleoprotein complex binding / protein targeting / : / cytoplasmic side of plasma membrane / protein transport / ribosome binding / zinc ion binding / ATP binding / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
SEC-C motif / SEC-C motif / Protein translocase subunit SecA / SecA DEAD-like, N-terminal / SecA Wing/Scaffold / SecA, preprotein cross-linking domain / SecA motor DEAD / SecA conserved site / SecA, Wing/Scaffold superfamily / SecA, preprotein cross-linking domain superfamily ...SEC-C motif / SEC-C motif / Protein translocase subunit SecA / SecA DEAD-like, N-terminal / SecA Wing/Scaffold / SecA, preprotein cross-linking domain / SecA motor DEAD / SecA conserved site / SecA, Wing/Scaffold superfamily / SecA, preprotein cross-linking domain superfamily / SecA, C-terminal helicase domain / SecA preprotein cross-linking domain / SecA Wing and Scaffold domain / SecA DEAD-like domain / SecA P-loop domain / SecA family signature. / SecA family profile. / SecA DEAD-like domain / SecA preprotein cross-linking domain / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Protein translocase subunit SecA
Similarity search - Component
Biological speciesEscherichia coli (strain K12) (bacteria)
CitationDate: 2019 Jun 27
Title: The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity
Authors: Jamshad M / Knowles T / White S / Ward D / Mohammed F / Rahman K / Wynne M / Hughes G / Kramer G / Bukau B
Contact author
  • Timothy Knowles (University of Birmingham, Birmingham, UK)

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Structure visualization

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Models

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Sample

SampleName: Protein translocase subunit SecA (full length, amino acids 1-901)
Specimen concentration: 5 mg/ml
BufferName: 20mM HEPES, 100mM NaCl, 1mM TCEP / pH: 8
Entity #1174Type: protein / Description: Protein translocase subunit SecA / Formula weight: 102.022 / Num. of mol.: 2 / Source: Escherichia coli (strain K12) / References: UniProt: P10408
Sequence: MLIKLLTKVF GSRNDRTLRR MRKVVNIINA MEPEMEKLSD EELKGKTAEF RARLEKGEVL ENLIPEAFAV VREASKRVFG MRHFDVQLLG GMVLNERCIA EMRTGEGKTL TATLPAYLNA LTGKGVHVVT VNDYLAQRDA ENNRPLFEFL GLTVGINLPG MPAPAKREAY ...Sequence:
MLIKLLTKVF GSRNDRTLRR MRKVVNIINA MEPEMEKLSD EELKGKTAEF RARLEKGEVL ENLIPEAFAV VREASKRVFG MRHFDVQLLG GMVLNERCIA EMRTGEGKTL TATLPAYLNA LTGKGVHVVT VNDYLAQRDA ENNRPLFEFL GLTVGINLPG MPAPAKREAY AADITYGTNN EYGFDYLRDN MAFSPEERVQ RKLHYALVDE VDSILIDEAR TPLIISGPAE DSSEMYKRVN KIIPHLIRQE KEDSETFQGE GHFSVDEKSR QVNLTERGLV LIEELLVKEG IMDEGESLYS PANIMLMHHV TAALRAHALF TRDVDYIVKD GEVIIVDEHT GRTMQGRRWS DGLHQAVEAK EGVQIQNENQ TLASITFQNY FRLYEKLAGM TGTADTEAFE FSSIYKLDTV VVPTNRPMIR KDLPDLVYMT EAEKIQAIIE DIKERTAKGQ PVLVGTISIE KSELVSNELT KAGIKHNVLN AKFHANEAAI VAQAGYPAAV TIATNMAGRG TDIVLGGSWQ AEVAALENPT AEQIEKIKAD WQVRHDAVLE AGGLHIIGTE RHESRRIDNQ LRGRSGRQGD AGSSRFYLSM EDALMRIFAS DRVSGMMRKL GMKPGEAIEH PWVTKAIANA QRKVESRNFD IRKQLLEYDD VANDQRRAIY SQRNELLDVS DVSETINSIR EDVFKATIDA YIPPQSLEEM WDIPGLQERL KNDFDLDLPI AEWLDKEPEL HEETLRERIL AQSIEVYQRK EEVVGAEMMR HFEKGVMLQT LDSLWKEHLA AMDYLRQGIH LRGYAQKDPK QEYKRESFSM FAAMLESLKY EVISTLSKVQ VRMPEEVEEL EQQRRMEAER LAQMQQLSHQ DDDSAAAAAL AAQTGERKVG RNDPCPCGSG KKYKQCHGRL Q

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.0919 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Protein translocase subunit SecA (full length, amino acids 1-901)
Measurement date: Jul 18, 2016 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 45 / Unit: 1/nm /
MinMax
Q0.0278 4.9518
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 366 /
MinMax
Q0.1696 1.89442
P(R) point1 366
R0 14.89
Result
Type of curve: sec
Comments: Additional SEC parameters: Column type, S200 10/300 GL (GE Lifesciences); Flow rate, 1.0mL/min; Sample injection concentration, 5mg/mL; Injection volume, 0.5mL
ExperimentalStandardPorod
MW232.267 kDa224.7 kDa-
Volume--424 nm3

P(R)GuinierGuinier error
Forward scattering, I0144 144.47 0.076
Radius of gyration, Rg4.186 nm4.22 nm-

MinMax
D-14.89
Guinier point23 60

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