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- SASDDX9: PAS fold family protein mPAC-Δ132 with the addition of adenosine-... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDDX9
SamplePAS fold family protein mPAC-Δ132 with the addition of adenosine-5'-[(α,β)-methyleno]triphosphate in the dark state (mPAC-Δ132, dark + ApCpp)
  • PAS fold family (protein), mPAC, Coleofasciculus chthonoplastes PCC 7420
Function / homology
Function and homology information


cyclic nucleotide biosynthetic process / adenylate cyclase activity / intracellular signal transduction
Similarity search - Function
PAS fold-3 / Adenylyl cyclase class-4/guanylyl cyclase, conserved site / Guanylate cyclase signature. / PAS fold / Adenylyl- / guanylyl cyclase, catalytic domain / Adenylyl cyclase class-3/4/guanylyl cyclase / Adenylate and Guanylate cyclase catalytic domain / Guanylate cyclase domain profile. / PAS domain / PAS-associated, C-terminal ...PAS fold-3 / Adenylyl cyclase class-4/guanylyl cyclase, conserved site / Guanylate cyclase signature. / PAS fold / Adenylyl- / guanylyl cyclase, catalytic domain / Adenylyl cyclase class-3/4/guanylyl cyclase / Adenylate and Guanylate cyclase catalytic domain / Guanylate cyclase domain profile. / PAS domain / PAS-associated, C-terminal / PAC domain profile. / Nucleotide cyclase / PAC motif / Motif C-terminal to PAS motifs (likely to contribute to PAS structural domain) / PAS domain / PAS repeat profile. / PAS domain / PAS domain superfamily
Similarity search - Domain/homology
Biological speciesColeofasciculus chthonoplastes PCC 7420 (bacteria)
Contact author
  • Robert Lindner (Max Planck Institute for Medical Research, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2062
Type: atomic / Chi-square value: 16.4811533492
Model #2157
Type: dummy / Software: (2.8.1) / Radius of dummy atoms: 3.25 A / Chi-square value: 52.36
Model #2158
Type: dummy / Software: (2.8.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 52.36

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Sample

SampleName: PAS fold family protein mPAC-Δ132 with the addition of adenosine-5'-[(α,β)-methyleno]triphosphate in the dark state (mPAC-Δ132, dark + ApCpp)
Specimen concentration: 5.10-9.10
BufferName: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 5 % w/v Glycerol, 1 mM ApCpp
pH: 7.5
Entity #1098Name: mPAC / Type: protein / Description: PAS fold family / Formula weight: 39.015 / Num. of mol.: 2 / Source: Coleofasciculus chthonoplastes PCC 7420 / References: UniProt: B4VKN6
Sequence: GAMGSQAYRK GVQETVKLRD QAIAASSVGI VIADARLPDM PLIYVNPAFE EITGYSDAEV LGYNCRFLQG KDTSQPAVDQ LRAAIKAGEN CTVTLLNYRK DGTPFWNELT ISPIYDDHNN LTHFVGIQSD ISDRIKAEQA LRLEQEKSER LLLNILPKPI VDQLKQFEGS ...Sequence:
GAMGSQAYRK GVQETVKLRD QAIAASSVGI VIADARLPDM PLIYVNPAFE EITGYSDAEV LGYNCRFLQG KDTSQPAVDQ LRAAIKAGEN CTVTLLNYRK DGTPFWNELT ISPIYDDHNN LTHFVGIQSD ISDRIKAEQA LRLEQEKSER LLLNILPKPI VDQLKQFEGS LAQQFTEATI LFADIVGFTQ LSAQMSPLEL LNLLNNIFSV FDKLAEKHGI EKIKTIGDAY MAVAGLPVAN DHHAEAIANM ALDMQQAIQQ FKTPQGEPFQ IRIGINTGLV VAGVIGIKKF SYDLWGDAVN VASRMESSGL PGKIQVTAAT KERLQDKYVF EKRGAIVVKG KGEMINYWLV GKQE

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Experimental information

BeamInstrument name: Swiss Light Source cSAXS / City: Villigen / 国: Switzerland / Type of source: X-ray synchrotron / Wavelength: 0.11 Å / Dist. spec. to detc.: 2.13 mm
DetectorName: Pilatus 2M
Scan
Title: mPAC_d132 dark +ApCpp (PAS fold family with the addition of Adenosine-5'-[(α,β)-methyleno]triphosphate)
Measurement date: Mar 11, 2015 / Cell temperature: 10 °C / Exposure time: 0.5 sec. / Number of frames: 200 / Unit: 1/A /
MinMax
Q0.016 0.2744
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 525 /
MinMax
Q0.0283825 0.268039
P(R) point1 525
R0 128
Result
Type of curve: merged /
ExperimentalPorod
MW83 kDa-
Volume-99 nm3

P(R)GuinierGuinier error
Forward scattering, I07.849 8.29 0.017
Radius of gyration, Rg3.81 nm3.95 nm0.19

MinMax
D-12.8
Guinier point12 35

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