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Yorodumi- SASDDU5: Nonstructural protein sigma NS - apoprotein (Nonstructural protei... -
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-Basic information
Entry | Database: SASBDB / ID: SASDDU5 |
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Sample | Nonstructural protein sigma NS - apoprotein
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Function / homology | Reovirus non-structural protein sigma NS / Sigma NS protein / single-stranded RNA binding / RNA-dependent RNA polymerase activity / Nonstructural protein sigma NS Function and homology information |
Biological species | Avian orthoreovirus |
Citation | Journal: Nucleic Acids Res / Year: 2018 Title: Stability of local secondary structure determines selectivity of viral RNA chaperones. Authors: Jack P K Bravo / Alexander Borodavka / Anders Barth / Antonio N Calabrese / Peter Mojzes / Joseph J B Cockburn / Don C Lamb / Roman Tuma / Abstract: To maintain genome integrity, segmented double-stranded RNA viruses of the Reoviridae family must accurately select and package a complete set of up to a dozen distinct genomic RNAs. It is thought ...To maintain genome integrity, segmented double-stranded RNA viruses of the Reoviridae family must accurately select and package a complete set of up to a dozen distinct genomic RNAs. It is thought that the high fidelity segmented genome assembly involves multiple sequence-specific RNA-RNA interactions between single-stranded RNA segment precursors. These are mediated by virus-encoded non-structural proteins with RNA chaperone-like activities, such as rotavirus (RV) NSP2 and avian reovirus σNS. Here, we compared the abilities of NSP2 and σNS to mediate sequence-specific interactions between RV genomic segment precursors. Despite their similar activities, NSP2 successfully promotes inter-segment association, while σNS fails to do so. To understand the mechanisms underlying such selectivity in promoting inter-molecular duplex formation, we compared RNA-binding and helix-unwinding activities of both proteins. We demonstrate that octameric NSP2 binds structured RNAs with high affinity, resulting in efficient intramolecular RNA helix disruption. Hexameric σNS oligomerizes into an octamer that binds two RNAs, yet it exhibits only limited RNA-unwinding activity compared to NSP2. Thus, the formation of intersegment RNA-RNA interactions is governed by both helix-unwinding capacity of the chaperones and stability of RNA structure. We propose that this protein-mediated RNA selection mechanism may underpin the high fidelity assembly of multi-segmented RNA genomes in Reoviridae. |
Contact author |
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-Structure visualization
-Downloads & links
-Data source
SASBDB page | SASDDU5 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Models
-Sample
Sample | Name: Nonstructural protein sigma NS - apoprotein |
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Buffer | Name: 25 mM HEPES, 150 mM NaCl / pH: 7.5 |
Entity #1026 | Type: protein / Description: Nonstructural protein sigma NS / Formula weight: 40.586 / Num. of mol.: 6 / Source: Avian orthoreovirus / References: UniProt: Q9DH28 Sequence: MDNTVRVGVS RNTSGAAGQT LFRNFYLLRC NISADGRNAT KAVQSHFPFL SRAVRCLSPL AAHCADRTLR RDNVKQILTR ELPFSSDLIN YAHHVNSSSL TTSQGVEAAR LVAQVYGEQV PFDHIYPTGS ATYCPGAIAN AISRIMAGFV PREGDDFAPS GPIDYLAADL ...Sequence: MDNTVRVGVS RNTSGAAGQT LFRNFYLLRC NISADGRNAT KAVQSHFPFL SRAVRCLSPL AAHCADRTLR RDNVKQILTR ELPFSSDLIN YAHHVNSSSL TTSQGVEAAR LVAQVYGEQV PFDHIYPTGS ATYCPGAIAN AISRIMAGFV PREGDDFAPS GPIDYLAADL IAYKFVLPYM LDMVDGRPQI VLPSHTVEEM LTNTSLLNSI DASFGIEARS DQRMTRDAAE MSSRSLNELE DHDQRGRMPW KIMLAMMAAQ LKVELDALAD ERTESQANAH VTSFGSRLFN QMSAFVTIDR ELMELALLIK EQGFAMNPGQ IASKWSLIRR SGPTRPLSGA RLEIRNGNWM IREGDQTLLS VSPARMA |
-Experimental information
Beam | Instrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 3.9 mm | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||||||
Scan | Title: Nonstructural protein sigma NS - apoprotein / Measurement date: Feb 25, 2017 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 1081 /
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Result | Type of curve: single_conc Comments: Sample concentration, UNKNOWN; Sample temperature, UNKNOWN; Exposure time; UNKNOWN.
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