+
データを開く
-
基本情報
登録情報 | ![]() |
---|---|
![]() | Human Rev7 dimer in complex with Rev3 peptide @ 12.2 mg/mL
|
機能・相同性 | ![]() somatic diversification of immunoglobulins involved in immune response / DNA damage response, signal transduction resulting in transcription / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: Rev7 dimerization is important for assembly and function of the Rev1/Polζ translesion synthesis complex. 著者: Alessandro A Rizzo / Faye-Marie Vassel / Nimrat Chatterjee / Sanjay D'Souza / Yunfeng Li / Bing Hao / Michael T Hemann / Graham C Walker / Dmitry M Korzhnev / ![]() 要旨: The translesion synthesis (TLS) polymerases Polζ and Rev1 form a complex that enables replication of damaged DNA. The Rev7 subunit of Polζ, which is a multifaceted HORMA (Hop1, Rev7, Mad2) protein ...The translesion synthesis (TLS) polymerases Polζ and Rev1 form a complex that enables replication of damaged DNA. The Rev7 subunit of Polζ, which is a multifaceted HORMA (Hop1, Rev7, Mad2) protein with roles in TLS, DNA repair, and cell-cycle control, facilitates assembly of this complex by binding Rev1 and the catalytic subunit of Polζ, Rev3. Rev7 interacts with Rev3 by a mechanism conserved among HORMA proteins, whereby an open-to-closed transition locks the ligand underneath the "safety belt" loop. Dimerization of HORMA proteins promotes binding and release of this ligand, as exemplified by the Rev7 homolog, Mad2. Here, we investigate the dimerization of Rev7 when bound to the two Rev7-binding motifs (RBMs) in Rev3 by combining in vitro analyses of Rev7 structure and interactions with a functional assay in a Rev7 cell line. We demonstrate that Rev7 uses the conventional HORMA dimerization interface both to form a homodimer when tethered by the two RBMs in Rev3 and to heterodimerize with other HORMA domains, Mad2 and p31 Structurally, the Rev7 dimer can bind only one copy of Rev1, revealing an unexpected Rev1/Polζ architecture. In cells, mutation of the Rev7 dimer interface increases sensitivity to DNA damage. These results provide insights into the structure of the Rev1/Polζ TLS assembly and highlight the function of Rev7 homo- and heterodimerization. |
![]() |
|
-
構造の表示
-
ダウンロードとリンク
-Data source
SASBDBのページ | ![]() |
---|
-関連構造データ
関連構造データ | ![]() 6bc8C ![]() 6bcdC ![]() 6bi7C C: 同じ文献を引用 ( |
---|---|
類似構造データ | 類似検索 - 機能・相同性 ![]() |
-
外部リンク
「今月の分子」の関連する項目 |
---|
-モデル
-
試料
![]() | 名称: Human Rev7 dimer in complex with Rev3 peptide @ 12.2 mg/mL 試料濃度: 12.2 mg/ml / Entity id: 857 / 858 / 859 |
---|---|
バッファ | 名称: 20 mM HEPES, 10 mM DTT, 5% glycerol / pH: 8 |
要素 #857 | 名称: hRev7-WT dimer / タイプ: protein / 記述: Mitotic spindle assembly checkpoint protein MAD2B / 分子量: 24.391 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9UI95 配列: GMTTLTRQDL NFGQVVADVL CEFLEVAVHL ILYVREVYPV GIFQKRKKYN VPVQMSCHPE LNQYIQDTLH CVKPLLEKND VEKVVVVILD KEHRPVEKFV FEITQPPLLS ISSDSLLSHV EQLLRAFILK ISVCDAVLDH NPPGCTFTVL VHTREAATRN MEKIQVIKDF ...配列: GMTTLTRQDL NFGQVVADVL CEFLEVAVHL ILYVREVYPV GIFQKRKKYN VPVQMSCHPE LNQYIQDTLH CVKPLLEKND VEKVVVVILD KEHRPVEKFV FEITQPPLLS ISSDSLLSHV EQLLRAFILK ISVCDAVLDH NPPGCTFTVL VHTREAATRN MEKIQVIKDF PWILADEQDV HMHDPRLIPL KTMTSDILKM QLYVEERAHK GS |
要素 #858 | 名称: Rev3-RBM2 / タイプ: protein / 記述: DNA polymerase zeta catalytic subunit / 分子量: 3.273 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: O60673 配列: MEDKKIVIMP CKCAPSRQLV QVWLQAKE |
要素 #859 | 名称: Rev3-RBM2 / タイプ: protein / 記述: DNA polymerase zeta catalytic subunit / 分子量: 3.273 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: O60673 配列: MEDKKIVIMP CKCAPSRQLV QVWLQAKE |
-実験情報
ビーム | 設備名称: Cornell High Energy Synchrotron Source (CHESS) G1 地域: Ithaca, NY / 国: USA ![]() ![]() | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: Finger Lakes CCD / タイプ: CCD | |||||||||||||||||||||||
スキャン | 測定日: 2016年5月14日 / 保管温度: 4 °C / セル温度: 4 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/A /
| |||||||||||||||||||||||
結果 | カーブのタイプ: single_conc コメント: Intra-particle repulsion was observed. This dataset was not used for modeling
|