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- SASDCZ4: RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex -

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Basic information

Entry
Database: SASBDB / ID: SASDCZ4
SampleRNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex
  • RNase E 603-850 (protein), RNase E 603-850, Escherichia coli
  • ATP-dependent RNA helicase RhlB (protein), RhlB, Escherichia coli
Function / homology
Function and homology information


: / RNA catabolic process / RNA helicase activity / RNA helicase / RNA binding / ATP binding / cytoplasm
Similarity search - Function
ATP-dependent RNA helicase RhlB type / DEAD-box subfamily ATP-dependent helicases signature. / RNA helicase, DEAD-box type, Q motif / ATP-dependent RNA helicase DEAD-box, conserved site / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. ...ATP-dependent RNA helicase RhlB type / DEAD-box subfamily ATP-dependent helicases signature. / RNA helicase, DEAD-box type, Q motif / ATP-dependent RNA helicase DEAD-box, conserved site / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
RNase E / ATP-dependent RNA helicase RhlB
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
CitationDate: 2017 Nov 9
Title: Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes
Authors: Bruce H / Du D / Matak-Vinkovic D / Bandyra K / Broadhurst R / Martin E / Sobott F / Shkumatov A
Contact author
  • Alexander Shkumatov (VUB, Vrije Universiteit Brussel, Pleinlaan 2 1050 Brussel)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1313
Type: dummy / Software: (2.3i r9450) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Best fitting model out of 10 independent reconstructions
Chi-square value: 16.39 / P-value: 0.000001
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex
Specimen concentration: 22.3 mg/ml / Entity id: 697 / 698
BufferName: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v)
pH: 7.5
Entity #697Name: RNase E 603-850 / Type: protein / Description: RNase E 603-850 / Formula weight: 30.127 / Num. of mol.: 1 / Source: Escherichia coli / References: UniProt: Q46977
Sequence: ERQQDRRKPR QNNRRDRNER RDTRSERTEG SDNREENRRN RRQAQQQTAE TRESRQQAEV TEKARTADEQ QAPRRERSRR RNDDKRQAQQ EAKALNVEEQ SVQETEQEER VRPVQPRRKQ RQLNQKVRYE QSVAEEAVVA PVVEETVAAE PIVQEAPAPR TELVKVPLPV ...Sequence:
ERQQDRRKPR QNNRRDRNER RDTRSERTEG SDNREENRRN RRQAQQQTAE TRESRQQAEV TEKARTADEQ QAPRRERSRR RNDDKRQAQQ EAKALNVEEQ SVQETEQEER VRPVQPRRKQ RQLNQKVRYE QSVAEEAVVA PVVEETVAAE PIVQEAPAPR TELVKVPLPV VAQTAPEQQE ENNADNRDNG GMPRRSRRSP RHLRVSGQRR RRYRDERYPT QSPMPLTVAC ASPELASGKV WIRYPIVRHH HHHH
Entity #698Name: RhlB / Type: protein / Description: ATP-dependent RNA helicase RhlB / Formula weight: 47.125 / Num. of mol.: 1 / Source: Escherichia coli / References: UniProt: W8ZZD4
Sequence: MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM ...Sequence:
MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT RPRTGNGPRR TGAPRNRRRS G

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1022 Å / Dist. spec. to detc.: 1.79 mm
DetectorName: AVIEX / Type: CCD
Scan
Title: RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex
Measurement date: Feb 11, 2016 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 1.5 sec. / Number of frames: 250 / Unit: 1/A /
MinMax
Q0.0062 0.6139
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 875 /
MinMax
Q0.0090467 0.501936
P(R) point1 875
R0 295
Result
Type of curve: other /
ExperimentalPorod
MW139 kDa107.7 kDa
Volume-183 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.217258 0.01 0.2172 0.01
Radius of gyration, Rg5.875 nm0.015 5.375 nm0.071

MinMaxError
D-29.5 1
Guinier point8 25 -

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