[English] 日本語
![](img/lk-miru.gif)
- SASDCM3: Atg17-Atg31-Atg29 Subcomplex (Autophagy-related protein 17, Atg17... -
+
Open data
-
Basic information
Entry | ![]() |
---|---|
![]() | Atg17-Atg31-Atg29 Subcomplex
|
Function / homology | ![]() positive regulation of autophagosome size / autophagy of peroxisome / : / vacuole-isolation membrane contact site / Atg1/ULK1 kinase complex / positive regulation of autophagosome assembly / autophagy of mitochondrion / protein localization to phagophore assembly site / phagophore assembly site membrane / piecemeal microautophagy of the nucleus ...positive regulation of autophagosome size / autophagy of peroxisome / : / vacuole-isolation membrane contact site / Atg1/ULK1 kinase complex / positive regulation of autophagosome assembly / autophagy of mitochondrion / protein localization to phagophore assembly site / phagophore assembly site membrane / piecemeal microautophagy of the nucleus / phagophore assembly site / protein kinase activator activity / SNARE binding / autophagy / protein transport / molecular adaptor activity Similarity search - Function |
Biological species | ![]() |
![]() | ![]() Title: Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Authors: Jürgen Köfinger / Michael J Ragusa / Il-Hyung Lee / Gerhard Hummer / James H Hurley / ![]() ![]() Abstract: The biogenesis of autophagosomes commences at the phagophore assembly site (PAS), a protein-vesicle ultrastructure that is organized by the Atg1 complex. The Atg1 complex consists of the Atg1 ...The biogenesis of autophagosomes commences at the phagophore assembly site (PAS), a protein-vesicle ultrastructure that is organized by the Atg1 complex. The Atg1 complex consists of the Atg1 protein kinase, the intrinsically disordered region-rich Atg13, and the dimeric double crescent-shaped Atg17-Atg31-Atg29 subcomplex. We show that the PAS contains a relatively uniform ∼28 copies of Atg17, and upon autophagy induction, similar numbers of Atg1 and Atg13 molecules. We then apply ensemble refinement of small-angle X-ray scattering to determine the solution structures of the Atg1-Atg13 and Atg17-Atg31-Atg29 subcomplexes and the Atg1 complex, using a trimmed minipentamer tractable to biophysical studies. We observe tetramers of Atg1 pentamers that assemble via Atg17-Atg31-Atg29. This leads to a model for the higher organization of the Atg1 complex in PAS scaffolding. |
![]() |
|
-
Structure visualization
-
Downloads & links
-Data source
SASBDB page | ![]() |
---|
-Related structure data
Related structure data | C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
External links
Related items in Molecule of the Month |
---|
-Models
-
Sample
![]() | Name: Atg17-Atg31-Atg29 Subcomplex / Specimen concentration: 1.00-5.30 / Entity id: 635 / 636 / 637 |
---|---|
Buffer | Name: 20 mM Tris, 200 mM NaCl, 2% glycerol / pH: 8 |
Entity #635 | Name: Atg17 / Type: protein / Description: Autophagy-related protein 17 / Formula weight: 49.864 / Num. of mol.: 2 Source: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) References: UniProt: Q6CS99 Sequence: MSDAATTIPS DEIDKLWNKA RNQLVEAQVE CEESLKILSK VRTEMDSSQK SRFKLKFILN CLVNQVEFFK NIMLEKCIST ELIDNEWSKL VLVEIVNDVS YWQNEITTKM KILQGTKYDL TDDHSSLSDF ICMDHVDILQ QKIDEIPIIK QQVTNIRQHY KSIKDRIENQ ...Sequence: MSDAATTIPS DEIDKLWNKA RNQLVEAQVE CEESLKILSK VRTEMDSSQK SRFKLKFILN CLVNQVEFFK NIMLEKCIST ELIDNEWSKL VLVEIVNDVS YWQNEITTKM KILQGTKYDL TDDHSSLSDF ICMDHVDILQ QKIDEIPIIK QQVTNIRQHY KSIKDRIENQ LVAVKLKKLR TYFDSHFSRD SKNNLFKLLE SDYVTELNEF ENELADFLRS ITDHFDKCTI LKEQQIPLPD LKELFQIVKK DDTQLENIRE LVFETGLEVK AFSKKVNETI SEIMDKIGGF HLLASKIVTE LEKCEEYLSI FQKIANLVFV YKESCIKKIE QVQQLCEFYD KFKLGYKNLL RERDRRKATA LQMEKILKEC QEKLQALSDA DLDQRQQFLL ENGDYLPENI WPGYIDDMES MYSFEYSIHN VPN |
Entity #636 | Type: protein / Description: Autophagy-related protein 29 / Formula weight: 10.031 / Num. of mol.: 2 Source: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) References: UniProt: Q6CTU8 Sequence: MNNDNTKVYV RVAGKRPENF VETKPFIWDS RRDKILWTKI SKIDSLEDMD WQELGADLGA PEPFLKKRSY TLFQNQLKVL SNQID |
Entity #637 | Name: Atg31 / Type: protein / Description: KLLA0A10637p / Formula weight: 16.071 / Num. of mol.: 2 Source: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) References: UniProt: Q6CX74 Sequence: MESPVLLVKN ANAATNASNL GLLGQKQNSY TVDENTWFLN NISFIFEDDD PSQHDEDQLD YENVIIIDSE PSGTVNNVEM LSERWQLLAF DQNPRDNCIS LTVMNELSAD LGQNTDISGL DNLSKLYHER NAQIRKLLDS IAP |
-Experimental information
Beam | Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS) City: Berkeley, CA / 国: USA ![]() | |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Pilatus3 X 2M / Pixsize x: 172 mm | |||||||||||||||||||||||||||||||||
Scan |
| |||||||||||||||||||||||||||||||||
Distance distribution function P(R) |
| |||||||||||||||||||||||||||||||||
Result |
|