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- SASDBR6: Nucleolysin TIA-1 isoform p40 in complex with UUUUUACUCC RNA -

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Basic information

Entry
Database: SASBDB / ID: SASDBR6
SampleNucleolysin TIA-1 isoform p40 in complex with UUUUUACUCC RNA
  • Nucleolysin TIA-1 isoform p40 (protein), TIA-1, Homo sapiens
  • RNA (UUUUUACU) (RNA)
Function / homologyIsoform Short of Cytotoxic granule associated RNA binding protein TIA1
Function and homology information
Biological speciesHomo sapiens (human)
CitationDate: 2017 Feb 10
Title: TIA-1 RRM23 binding and recognition of target oligonucleotides
Authors: Waris S / García-Mauriño S / Sivakumaran A / Beckham S / Loughlin F / Gorospe M / Díaz-Moreno I / Wilce M
Contact author
  • Matthew Wilce (Monash University, Melbourne, Australia)

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Structure visualization

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Models

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Sample

SampleName: Nucleolysin TIA-1 isoform p40 in complex with UUUUUACUCC RNA
Specimen concentration: 2.50-2.50 / Entity id: 414 / 417
BufferName: 20 mM HEPES, 100mM NaCl, 3% v/v glycerol / pH: 7
Entity #414Name: TIA-1 / Type: protein / Description: Nucleolysin TIA-1 isoform p40 / Formula weight: 21.325 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: p31483-2
Sequence: ENLYFQGAMG NHFHVFVGDL SPEITTEDIK AAFAPFGRIS DARVVKDMAT GKSKGYGFVS FFNKWDAENA IQQMGGQWLG GRQIRTNWAT RKPPAPKSTY ESNTKQLSYD EVVNQSSPSN CTVYCGGVTS GLTEQLMRQT FSPFGQIMEI RVFPDKGYSF VRFNSHESAA ...Sequence:
ENLYFQGAMG NHFHVFVGDL SPEITTEDIK AAFAPFGRIS DARVVKDMAT GKSKGYGFVS FFNKWDAENA IQQMGGQWLG GRQIRTNWAT RKPPAPKSTY ESNTKQLSYD EVVNQSSPSN CTVYCGGVTS GLTEQLMRQT FSPFGQIMEI RVFPDKGYSF VRFNSHESAA HAIVSVNGTT IEGHVVKCYW GK
Entity #417Type: RNA / Description: RNA (UUUUUACU) / Formula weight: 0.942 / Num. of mol.: 1
Sequence:
UUUUUACUCC

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Experimental information

BeamInstrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / : Australia / Shape: Point / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 1.6 mm
DetectorName: Pilatus 1M
Scan
Title: Nucleolysin TIA-1 isoform p40 in complex with UUUU / Measurement date: May 27, 2016 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 1 sec. / Number of frames: 30 / Unit: 1/A /
MinMax
Q0.0064 0.3615
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 457 /
MinMax
Q0.0232222 0.201113
P(R) point1 457
R0 65
Result
Type of curve: single_conc / Comments: TIA-1 RRM23/UCrich RNA /
ExperimentalPorod
MW23.77 kDa-
Volume-26.48 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.05606 0.0001 0.056 0.00013
Radius of gyration, Rg2.1 nm0.004 2.09 nm-

MinMax
D-6.5
Guinier point44 131

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