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- SASDBN9: Putative DNA binding protein YeeF-CT in complex with the cognate ... -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDBN9
SamplePutative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis
  • Putative DNA binding protein (protein), YeeF, Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
  • YezG, cognate immunity protein of YeeF (protein), YezG, Bacillus subtilis subsp. spizizenii str. W23
Function / homology
Function and homology information


LXG domain of WXG superfamily / Immunity protein YezG-like / LXG domain / Immunity protein YezG-like superfamily / Type VII secretion system protein EssD-like / Immunity protein YezG-like / LXG domain profile. / DNA/RNA non-specific endonuclease / DNA/RNA non-specific endonuclease superfamily
Similarity search - Domain/homology
Immunity protein YezG / Putative DNA binding protein
Similarity search - Component
Biological speciesBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) (bacteria)
Bacillus subtilis subsp. spizizenii str. W23
Contact author
  • Krishan Gopal (CSIR-INSTITUTE OF MICROBIAL TECHNOLOGY, Chandigarh, India)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1104
Type: dummy / Software: DAMMIN and DAMAVER (2.7.2) / Radius of dummy atoms: 4.00 A / Chi-square value: 0.812

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Sample

SampleName: Putative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis
Specimen concentration: 7.5 mg/ml / Entity id: 573 / 574
BufferName: 20 mM HEPES 150 mM NaCl / pH: 7.5
Entity #573Name: YeeF / Type: protein / Description: Putative DNA binding protein / Formula weight: 18.402 / Num. of mol.: 2
Source: Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
References: UniProt: E0TU96_BAC
Sequence:
MGSSHHHHHH SSGLVPRSHM ASIYKTKEGY TYTTDNYGRI TSVKADLQLG EAKRNQYAQS HAGKPQDRKP DDDGGALIAT QFKGSGQFDN IVPMNSQINR SGGRWYEMEQ EWAKALKEEP PQKVNVNIKA IYKGDSLRPD KFIVKFRIGD ADFEKVTIKN QSGG
Entity #574Name: YezG / Type: protein / Description: YezG, cognate immunity protein of YeeF / Formula weight: 19.234 / Num. of mol.: 1 / Source: Bacillus subtilis subsp. spizizenii str. W23 / References: UniProt: E0TU95
Sequence:
MDTPKMGDLY QRIANQINEM IPSEWENVYL YAEILDDSSE VYFYFNIPGK NEFLYSHNIP EHFNVSEDIY DDLLIELQES FEELREEYEK NNPETWTNLT LKLDRTGQFS IDYNYEDVIA SELNGSQRKA VWVYKNLGLM PKRKTVRDFL EDYIKTNEGK I

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Experimental information

BeamInstrument name: IMTECH / City: Chandigarh / 国: India / Type of source: X-ray in house / Wavelength: 0.15418 Å
DetectorName: 1D CMOS Mythen detector / Type: Diode
Scan
Title: Putative DNA binding protein YeeF-CT (528-669) in complex with the cognate immunity protein YezG from Bacillus subtilis
Measurement date: Aug 18, 2016 / Storage temperature: 4 °C / Cell temperature: 25 °C / Exposure time: 1800 sec. / Number of frames: 1 / Unit: 1/nm /
MinMax
Q0.1206 7.0014
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 290 /
MinMax
Q0.133373 1.98015
P(R) point1 290
R0 14.1
Result
Type of curve: single_conc /
ExperimentalPorod
MW72.03 kDa72.03 kDa
Volume-115.26 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I072150 1510 72749 2036
Radius of gyration, Rg4.15 nm0.09 4.04 nm0.18

MinMax
D-14.1
Guinier point2 32

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