SASDBH6: Full-length human p23 (1-160) (Prostaglandin E synthase 3, PTGES,...

基本情報

登録情報

データベース: SASBDB / ID: SASDBH6

試料

Full-length human p23 (1-160)

• Prostaglandin E synthase 3 (protein), PTGES, Sba1, p23, Homo sapiens

機能・相同性

分子機能:

lung saccule development / prostaglandin-E synthase / prostaglandin-E synthase activity / nuclear receptor-mediated glucocorticoid signaling pathway / prostanoid biosynthetic process / Aryl hydrocarbon receptor signalling / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / glycogen biosynthetic process / telomerase holoenzyme complex / protein folding chaperone complex / prostaglandin biosynthetic process / skin development / telomerase holoenzyme complex assembly / : / HSF1 activation / telomere maintenance via telomerase / Attenuation phase / chaperone-mediated protein complex assembly / DNA polymerase binding / positive regulation of telomere maintenance via telomerase / telomere maintenance / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / ESR-mediated signaling / Hsp90 protein binding / telomerase activity / unfolded protein binding / protein folding / protein-folding chaperone binding / fibroblast proliferation / Estrogen-dependent gene expression / Potential therapeutics for SARS / chromosome, telomeric region / protein stabilization / signal transduction / protein-containing complex / nucleoplasm / nucleus / cytosol

ドメイン・相同性:

Co-chaperone protein p23-like / CS domain / CS domain / CS domain profile. / HSP20-like chaperone

構成要素:

Prostaglandin E synthase 3

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: Arch Biochem Biophys / 年: 2015; タイトル: The C-terminal region of the human p23 chaperone modulates its structure and function.; 著者: Thiago V Seraphim / Lisandra M Gava / David Z Mokry / Thiago C Cagliari / Leandro R S Barbosa / Carlos H I Ramos / Júlio C Borges / ; 要旨: The p23 protein is a chaperone widely involved in protein homeostasis, well known as an Hsp90 co-chaperone since it also controls the Hsp90 chaperone cycle. Human p23 includes a β-sheet domain, responsible for interacting with Hsp90; and a charged C-terminal region whose function is not clear, but seems to be natively unfolded. p23 can undergo caspase-dependent proteolytic cleavage to form p19 (p231-142), which is involved in apoptosis, while p23 has anti-apoptotic activity. To better elucidate the function of the human p23 C-terminal region, we studied comparatively the full-length human p23 and three C-terminal truncation mutants: p23₁₋₁₁₇; p23₁₋₁₃₁ and p23₁₋₁₄₂. Our data indicate that p23 and p19 have distinct characteristics, whereas the other two truncations behave similarly, with some differences to p23 and p19. We found that part of the C-terminal region can fold in an α-helix conformation and slightly contributes to p23 thermal-stability, suggesting that the C-terminal interacts with the β-sheet domain. As a whole, our results suggest that the C-terminal region of p23 is critical for its structure-function relationship. A mechanism where the human p23 C-terminal region behaves as an activation/inhibition module for different p23 activities is proposed.

登録者

• Júlio Borges (Instituto de Química de São Carlos, São Carlos - SP - Brasil)

モデル

試料

• 試料: 名称: Full-length human p23 (1-160) / 試料濃度: 1.00-2.00
• バッファ: 名称: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol / pH: 7.5

要素 #404

名称: PTGES, Sba1, p23 / タイプ: protein / 記述: Prostaglandin E synthase 3 / 分子量: 18.841 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q15185
配列:

GSMQPASAKW YDRRDYVFIE FCVEDSKDVN VNFEKSKLTF SCLGGSDNFK HLNEIDLFHC IDPNDSKHKR TDRSILCCLR KGESGQSWPR LTKERAKLNW LSVDFNNWKD WEDDSDEDMS NFDRFSEMMN NMGGDEDVDL PEVDGADDDS QDSDDEKMPD LE

実験情報

• ビーム: 設備名称: Brazilian Synchrotron Light Laboratory SAXS1 Beamline; 地域: Campinas / 国: Brazil / 線源: X-ray synchrotron / 波長: 0.1488 Å / スペクトロメータ・検出器間距離: 1 mm
• 検出器: 名称: Pilatus 300K / タイプ: 20Hz

スキャン

タイトル: Full-length human p23 (1-160) / 測定日: 2012年6月22日 / 保管温度: 4 °C / セル温度: 20 °C / 照射時間: 30 sec. / フレーム数: 2 / 単位: 1/A /

	Min	Max
Q	0.0206	0.3684

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 278 /

	Min	Max
Q	0.02059	0.3684
P(R) point	1	278
R	0	100

結果

カーブのタイプ: single_conc
コメント: Samples were measured at 1 mg/mL and 2 mg/mL in 1 mm path-length mica cells. All curves were inspected for X-ray damage and aggregation. The experimental molecular weight was determined by analytical ultracentrifugation.

	実験値	Porod
分子量	21 kDa	-
体積	-	40 nm3

	P(R) error	Guinier	Guinier error	P(R)
前方散乱 I0	4.0E-5	0.019	6.0E-5	-
慣性半径, Rg	0.01	2.53 nm	0.12	2.69 nm

	Min	Max
D	-	10
Guinier point	3	26