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- SASDB64: Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-... -
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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDB64 |
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![]() | Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)
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Function / homology | ![]() |
Biological species | ![]() |
![]() | ![]() Title: Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Authors: Fernanda A H Batista / Thiago V Seraphim / Clelton A Santos / Marisvanda R Gonzaga / Leandro R S Barbosa / Carlos H I Ramos / Júlio C Borges / ![]() Abstract: Parasites belonging to the genus Leishmania are subjected to extensive environmental changes during their life cycle; molecular chaperones/co-chaperones act as protagonists in this scenario to ...Parasites belonging to the genus Leishmania are subjected to extensive environmental changes during their life cycle; molecular chaperones/co-chaperones act as protagonists in this scenario to maintain cellular homeostasis. Hop/Sti1 is a co-chaperone that connects the Hsp90 and Hsp70 systems, modulating their ATPase activities and affecting the fate of client proteins because it facilitates their transfer from the Hsp70 to the Hsp90 chaperone. Hop/Sti1 is one of the most prevalent co-chaperones, highlighting its importance despite the relatively low sequence identity among orthologue proteins. This multi-domain protein comprises three tetratricopeptides domains (TPR1, TPR2A and TPR2B) and two Asp/Pro-rich domains. Given the importance of Hop/Sti1 for the chaperone system and for Leishmania protozoa viability, the Leishmania braziliensis Hop (LbHop) and a truncated mutant (LbHop(TPR2AB)) were characterized. Structurally, both proteins are α-helix-rich and highly elongated monomeric proteins. Functionally, they inhibited the ATPase activity of Leishmania braziliensis Hsp90 (LbHsp90) to a similar extent, and the thermodynamic parameters of their interactions with LbHsp90 were similar, indicating that TPR2A-TPR2B forms the functional center for the LbHop interaction with LbHsp90. These results highlight the structural and functional similarity of Hop/Sti1 proteins, despite their low sequence conservation compared to the Hsp70 and Hsp90 systems, which are phylogenetic highly conserved. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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External links
Related items in Molecule of the Month |
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-Models
Model #483 | ![]() Type: dummy / Software: DAMFILT / Radius of dummy atoms: 1.90 A / Chi-square value: 1.749 / P-value: 0.203609 ![]() |
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Sample
![]() | Name: Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct) Specimen concentration: 1.40-8.50 |
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Buffer | Name: Tris / Concentration: 25.00 mM / pH: 7.5 Composition: 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol |
Entity #315 | Name: Truncated Hop/Sti1 / Type: protein Description: Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) Formula weight: 43.7 / Num. of mol.: 1 / Source: Leishmania braziliensis / References: UniProt: A4H5F0 Sequence: GSHMEDQRFA LTLMYLSGMK IPNDDEDDEE ERPSAKAAAE AKAKEEKKLL TDNEKEAMAL KEEGNKLYLS KRFEEALSKY QEAQAKDPKN TLYILNVSAV YFEQRDYEKC ITECERGIEH GRENHCDYTI VAKLMTRHAF CLQKQKKYEA AIDLYKRALV EWRNPDTLKK ...Sequence: GSHMEDQRFA LTLMYLSGMK IPNDDEDDEE ERPSAKAAAE AKAKEEKKLL TDNEKEAMAL KEEGNKLYLS KRFEEALSKY QEAQAKDPKN TLYILNVSAV YFEQRDYEKC ITECERGIEH GRENHCDYTI VAKLMTRHAF CLQKQKKYEA AIDLYKRALV EWRNPDTLKK LTECEKEHQK AVEEAYIDPE IARQKKDEGN QYFKEDKFPE AVAAYTEAIK RNPAEHTSYS NRAAAYIKLG AFNDALKDAE KCIELKPDFV KGYARKGHAY FWTKQYNRAL QAYDEGLKVD PSNADCKDGR LRTIMRIQEM ASGQSADGDE AARRAMDDPE IAAIMQDSYM QLVLKEMQND PTRIQEYMKD PGISVKINKL ISAGIIRFGQ |
-Experimental information
Beam | Instrument name: Brazilian Synchrotron Light Laboratory SAXS2 Beamline City: Campinas / 国: Brazil ![]() | |||||||||||||||||||||||||||||||||
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Detector | Name: MAR 165 CCD | |||||||||||||||||||||||||||||||||
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Distance distribution function P(R) |
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Result |
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