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- SASDAQ7: PBX1-PREB1-DNA complex (Pre-B-cell leukemia transcription factor ... -

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Basic information

Entry
Database: SASBDB / ID: SASDAQ7
SamplePBX1-PREB1-DNA complex
  • Pre-B-cell leukemia transcription factor 1 (protein), PBX1, Homo sapiens
  • Homeobox protein PKNOX1 (protein), PREP1, Homo sapiens
  • DNA 22mer (DNA)
Biological speciesHomo sapiens (human)

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Structure visualization

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Models

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Sample

SampleName: PBX1-PREB1-DNA complex / Specimen concentration: 3.8 mg/ml / Entity id: 160 / 161 / 162
BufferName: Tris-HCl / Concentration: 20.00 mM / pH: 7.4 / Composition: 150 mM NaCl, 5% glycerol, 1 mM DTT
Entity #160Name: PBX1 / Type: protein / Description: Pre-B-cell leukemia transcription factor 1 / Formula weight: 34.455 / Num. of mol.: 1 / Source: Homo sapiens
Sequence: MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY RAKLSQIRQI YHTELEKYEQ ...Sequence:
MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM NLLREQSRTR PISPKEIERM VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF NKQATEILNE YFYSHLSNPY PSEEAKEELA KKCGITVSQV SNWFGNKRIR YKKNIGKFQE EANIYAAK
Entity #161Name: PREP1 / Type: protein / Description: Homeobox protein PKNOX1 / Formula weight: 38.339 / Num. of mol.: 1 / Source: Homo sapiens
Sequence: MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTEMNSETLL SGEPGSPYSP ...Sequence:
MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTEMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRF
Entity #162Type: DNA / Description: DNA 22mer / Formula weight: 11.9 / Num. of mol.: 1
Sequence:
5GCCTAGTGA TTGACAGATT CCT3

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
ScanTitle: PREP1-PBX1-DNA / Measurement date: Dec 6, 2013 / Unit: 1/nm /
MinMax
Q0.0275 4.4504
ResultType of curve: single_conc
ExperimentalStandardPorod
MW80 kDa80 kDa136 kDa
Volume--173 nm3

P(R)Guinier
Forward scattering, I01613 1640
Radius of gyration, Rg4.8 nm4.8 nm

MinMax
D-16.3
Guinier point15 94

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