SASDAF8: Human Filamin A Ig-like domains 20-21 truncation (2151-2329)

基本情報

登録情報

データベース: SASBDB / ID: SASDAF8

試料

Human Filamin A Ig-like domains 20-21 truncation (2151-2329)

• Human Filamin A Ig-like domains 20-21 (protein), FilaminA, Homo sapiens

機能・相同性

分子機能:

regulation of membrane repolarization during atrial cardiac muscle cell action potential / regulation of membrane repolarization during cardiac muscle cell action potential / establishment of Sertoli cell barrier / formation of radial glial scaffolds / Myb complex / adenylate cyclase-inhibiting dopamine receptor signaling pathway / protein localization to bicellular tight junction / positive regulation of integrin-mediated signaling pathway / positive regulation of neuron migration / blood coagulation, intrinsic pathway / OAS antiviral response / actin crosslink formation / positive regulation of actin filament bundle assembly / tubulin deacetylation / megakaryocyte development / Cell-extracellular matrix interactions / positive regulation of platelet activation / positive regulation of potassium ion transmembrane transport / protein localization to cell surface / apical dendrite / positive regulation of neural precursor cell proliferation / Fc-gamma receptor I complex binding / podosome / negative regulation of transcription by RNA polymerase I / wound healing, spreading of cells / GP1b-IX-V activation signalling / receptor clustering / SMAD binding / cortical cytoskeleton / RHO GTPases activate PAKs / semaphorin-plexin signaling pathway / mitotic spindle assembly / potassium channel regulator activity / positive regulation of substrate adhesion-dependent cell spreading / cilium assembly / release of sequestered calcium ion into cytosol / regulation of cell migration / protein localization to plasma membrane / dendritic shaft / actin filament / establishment of protein localization / negative regulation of protein catabolic process / positive regulation of protein import into nucleus / cerebral cortex development / protein sequestering activity / platelet aggregation / mRNA transcription by RNA polymerase II / G protein-coupled receptor binding / kinase binding / small GTPase binding / Z disc / cell-cell junction / actin filament binding / actin cytoskeleton organization / actin cytoskeleton / Platelet degranulation / growth cone / GTPase binding / DNA-binding transcription factor binding / perikaryon / transmembrane transporter binding / positive regulation of canonical NF-kappaB signal transduction / postsynapse / protein stabilization / cadherin binding / focal adhesion / negative regulation of apoptotic process / nucleolus / perinuclear region of cytoplasm / glutamatergic synapse / protein homodimerization activity / RNA binding / extracellular exosome / extracellular region / membrane / nucleus / plasma membrane / cytosol / cytoplasm

ドメイン・相同性:

Filamin family / Filamin/ABP280 repeat / Filamin-type immunoglobulin domains / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. / Actinin-type actin-binding domain, conserved site / Calponin homology domain / Calponin homology (CH) domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Immunoglobulin E-set / Immunoglobulin-like fold

構成要素:

Filamin-A

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: PLoS One / 年: 2015; タイトル: Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21.; 著者: Jonne Seppälä / Helena Tossavainen / Nebojsa Rodic / Perttu Permi / Ulla Pentikäinen / Jari Ylänne / ; 要旨: Filamins (FLNs) are large, multidomain actin cross-linking proteins with diverse functions. Besides regulating the actin cytoskeleton, they serve as important links between the extracellular matrix and the cytoskeleton by binding cell surface receptors, functioning as scaffolds for signaling proteins, and binding several other cytoskeletal proteins that regulate cell adhesion dynamics. Structurally, FLNs are formed of an amino terminal actin-binding domain followed by 24 immunoglobulin-like domains (IgFLNs). Recent studies have demonstrated that myosin-mediated contractile forces can reveal hidden protein binding sites in the domain pairs IgFLNa18-19 and 20-21, enabling FLNs to transduce mechanical signals in cells. The atomic structures of these mechanosensor domain pairs in the resting state are known, as well as the structures of individual IgFLN21 with ligand peptides. However, little experimental data is available on how interacting protein binding deforms the domain pair structures. Here, using small-angle x-ray scattering-based modelling, x-ray crystallography, and NMR, we show that the adaptor protein migfilin-derived peptide-bound structure of IgFLNa20-21 is flexible and adopts distinctive conformations depending on the presence or absence of the interacting peptide. The conformational changes reported here may be common for all peptides and may play a role in the mechanosensor function of the site.

登録者

• Jonne Seppälä (University of Jyväskylä, Jyväskylän, Finland)

モデル

試料

• 試料: 名称: Human Filamin A Ig-like domains 20-21 truncation (2151-2329); 試料濃度: 1.00-4.00
• バッファ: 名称: Tris / 濃度: 20.00 mM / pH: 8 / 組成: 50 mM NaCl, 10mM DTT

要素 #188

名称: FilaminA / タイプ: protein / 記述: Human Filamin A Ig-like domains 20-21 / 分子量: 18.7 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P21333
配列:

SVANVGSHCD LSLKIPEISI QDMTAQVTSP SGKTHEAEIV EGENHTYCIR FVPAEMGTHT VSVKYKGQHV PGSPFQFTVG PLGEGGAHKV RAGGPGLERA EAGVPAEFSI WTREAGAGGL AIAVEGPSKA EISFEDRKDG SCGVAYVVQE PGDYEVSVKF NEEHIPDSPF VVPVASPS

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.93 Å / スペクトロメータ・検出器間距離: 2.43 mm
• 検出器: 名称: Pilatus 1M

スキャン

タイトル: Human Filamin A Ig-like domains 20-21 / 測定日: 2014年2月1日 / 保管温度: 20 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0649	4.61

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 260 /

	Min	Max
Q	0.1775	2.66
P(R) point	24	283
R	0	8.5

結果

カーブのタイプ: merged
コメント: Filamin A fragment residues 2151-2329 (Ig-like domains). NOTE: The displayed SAXS data and data used for P(r) vs r determination are on different I(s) scales.

	実験値	Porod	Estimated method
分子量	30 kDa	17 kDa	-
体積	-	28.9 nm3	Datporod

	P(R)	P(R) error	Guinier
前方散乱 I0	29.9	0.044	27
慣性半径, Rg	2.45 nm	0.006	2.43 nm

	Min	Max
D	-	8.5
Guinier point	22	76