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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDAB7 |
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![]() | Complex ComE-comcde
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機能・相同性 | ![]() |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. 著者: Dyana Sanchez / Marion Boudes / Herman van Tilbeurgh / Dominique Durand / Sophie Quevillon-Cheruel / ![]() 要旨: The ComD-ComE two-component system controls the competence state of Streptococcus pneumoniae via the phospho-regulation of ComE, which fluctuates between monomeric and dimeric states. We previously ...The ComD-ComE two-component system controls the competence state of Streptococcus pneumoniae via the phospho-regulation of ComE, which fluctuates between monomeric and dimeric states. We previously showed that the non-phosphorylatable ComE(D) (58A) mutant is monomeric in solution, whereas the ComE(D) (58E) active mimic mutant dimerizes via its REC domains. The crystal structure of ComE(D) (58A) revealed an asymmetric dimer that may represent the activated form of ComE. Here, we investigated the binding between the catalytic domain of ComD, ComE and the promoter region comcde, using small angle X-ray scattering. ComD(catdom) is a dimer that adapts two monomers of ComE, one on each side, placing (Com) (E) D58 residue in front of (Com) (D) H248, a location that is convenient for the intermolecular transfer reaction of the phosphoryl group. The LytTR, ComE(D) (58A) and ComE(D) (58E) complexed with comcde are composed of two protein molecules per DNA duplex. Modeling the complexes against small angle X-ray scattering data indicated that ComE(D) (58E) bound to comcde forms a compact dimer similar to the crystal structure, whereas ComE(D) (58A) -comcde adopts more than one conformation with or without dimer contacts. The various oligomeric states of ComE induce different bending angles of the promoter, which provides a mechanistic scenario for the activation of ComE: the phosphorylation of ComE forces additional bending of comcde, and the release of this bending strain on DNA via the disruption of the ComE dimer may signal the shut-off of the competence state. データベース: The molecular models and experimental SAXS data have been deposited on SASBDB (Small Angle Scattering Biological Data Bank) (see http://www.sasbdb.org/aboutSASBDB/) under the SAS ...データベース: The molecular models and experimental SAXS data have been deposited on SASBDB (Small Angle Scattering Biological Data Bank) (see http://www.sasbdb.org/aboutSASBDB/) under the SAS codes SASDAA7, SASDAB7 and SASDAC7. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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-モデル
モデル #253 | ![]() タイプ: atomic / カイ2乗値: 1.473796 ![]() |
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試料
![]() | 名称: Complex ComE-comcde / 試料濃度: 0.8 mg/ml / Entity id: 152 / 155 |
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バッファ | 名称: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol 濃度: 50.00 mM / pH: 6.2 組成: 500 mM NaCl, 5% (vol/vol) glycerol, 5 mM β-mercaptoethanol |
要素 #152 | タイプ: DNA / 記述: comcde / 分子量: 23.5 / 由来: Streptococcus pneumoniae 配列: 5AAGTACACT TTGGGAGAAA AAAATGACAG TTGAGAGAA3 3TTCATGTGA AACCCTCTTT TTTTACTGTC AACTCTCTT5 |
要素 #155 | 名称: ComE / タイプ: protein / 記述: Response regulator / 分子量: 30.736 / 分子数: 2 / 由来: Streptococcus pneumoniae / 参照: UniProt: Q8DMW5 配列: MKVLILEDVI EHQVRLERIL DEISKESNIP ISYKTTGKVR EFEEYIENDE VNQLYFLAID IHGIEKKGFE VAQLIRHYNP YAIIVFITSR SEFATLTYKY QVSALDFVDK DINDEMFKKR IEQNIFYTKS MLLENEDVVD YFDYNYKGND LKIPYHDILY IETTGVSHKL ...配列: MKVLILEDVI EHQVRLERIL DEISKESNIP ISYKTTGKVR EFEEYIENDE VNQLYFLAID IHGIEKKGFE VAQLIRHYNP YAIIVFITSR SEFATLTYKY QVSALDFVDK DINDEMFKKR IEQNIFYTKS MLLENEDVVD YFDYNYKGND LKIPYHDILY IETTGVSHKL RIIGKNFAKE FYGTMTDIQE KDKHTQRFYS PHKSFLVNIG NIREIDRKNL EIVFYEDHRC PISRLKIRKL KDILEKKSQK HHHHHH |
-実験情報
ビーム | 設備名称: SOLEIL SWING ![]() ![]() | ||||||||||||||||||
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検出器 | 名称: AVIEX / タイプ: CCD | ||||||||||||||||||
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距離分布関数 P(R) |
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結果 | コメント: Use of SAXS to describe the interaction between the phosphorylated mimetic mutant ComED58E of the response regulator ComE from S. pneumonia and its promoter region comcde. The complex ...コメント: Use of SAXS to describe the interaction between the phosphorylated mimetic mutant ComED58E of the response regulator ComE from S. pneumonia and its promoter region comcde. The complex is constituted of one dimer of ComE and one monomer of comcde. The SAXS data were collected directly after elution through an on-line size-exclusion high-performance liquid chromatography (SEC-HPLC) column. The molecular weight is not estimated from I(0) because the concentration of the complex cannot be accurately determined. It is obtained from the macromolecule volume using the method developed by Craievich’s team (the SAXS Mow program).
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