+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDA25 |
---|---|
試料 | fh1213
|
生物種 | Homo sapiens (ヒト) |
引用 | ジャーナル: J Mol Biol / 年: 2010 タイトル: The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. 著者: Christoph Q Schmidt / Andrew P Herbert / Haydyn D T Mertens / Mara Guariento / Dinesh C Soares / Dusan Uhrin / Arthur J Rowe / Dmitri I Svergun / Paul N Barlow / 要旨: The first eight and the last two of 20 complement control protein (CCP) modules within complement factor H (fH) encompass binding sites for C3b and polyanionic carbohydrates. These binding sites ...The first eight and the last two of 20 complement control protein (CCP) modules within complement factor H (fH) encompass binding sites for C3b and polyanionic carbohydrates. These binding sites cooperate self-surface selectively to prevent C3b amplification, thus minimising complement-mediated damage to host. Intervening fH CCPs, apparently devoid of such recognition sites, are proposed to play a structural role. One suggestion is that the generally small CCPs 10-15, connected by longer-than-average linkers, act as a flexible tether between the two functional ends of fH; another is that the long linkers induce a 180 degrees bend in the middle of fH. To test these hypotheses, we determined the NMR-derived structure of fH12-13 consisting of module 12, shown here to have an archetypal CCP structure, and module 13, which is uniquely short and features a laterally protruding helix-like insertion that contributes to a prominent electropositive patch. The unusually long fH12-13 linker is not flexible. It packs between the two CCPs that are not folded back on each other but form a shallow vee shape; analytical ultracentrifugation and X-ray scattering supported this finding. These two techniques additionally indicate that flanking modules (within fH11-14 and fH10-15) are at least as rigid and tilted relative to neighbours as are CCPs 12 and 13 with respect to one another. Tilts between successive modules are not unidirectional; their principal axes trace a zigzag path. In one of two arrangements for CCPs 10-15 that fit well with scattering data, CCP 14 is folded back onto CCP 13. In conclusion, fH10-15 forms neither a flexible tether nor a smooth bend. Rather, it is compact and has embedded within it a CCP module (CCP 13) that appears to be highly specialised given both its deviant structure and its striking surface charge distribution. A passive, purely structural role for this central portion of fH is unlikely. |
登録者 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-モデル
モデル #81 | タイプ: atomic / ソフトウェア: crysol / カイ2乗値: 2.019241 Omokage検索でこの集合体の類似形状データを探す (詳細) |
---|---|
モデル #82 | タイプ: dummy / ソフトウェア: dammif / ダミー原子の半径: 1.90 A / カイ2乗値: 2.493241 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #83 | タイプ: dummy / ソフトウェア: gasbor / ダミー原子の半径: 1.90 A / カイ2乗値: 3.0625 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: fh1213 / Sample MW: 13.6 kDa / 試料濃度: 0.70-3.50 / 濃度測定法: A280 |
---|---|
バッファ | 名称: Potassium Phosphate / 濃度: 50.00 mM / pH: 7.4 |
要素 #73 | 名称: fH1213 / タイプ: protein / 記述: Factor H CCP modules 12 to 13 / 分子量: 13.6 / 分子数: 1 / 由来: Homo sapiens 配列: EAAGTCGDIP ELEHGWAQLS SPPYYYGDSV EFNCSESFTM IGHRSITCIH GVWTQLPQCV AIDKLKKCKS SNLIILEEHL KNKKEFDHNS NIRYRCRGKE GWIHTVCING RWDPEVNCS |
-実験情報
ビーム | 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: MAR 345 Image Plate | ||||||||||||||||||||||||||||||
スキャン | タイトル: fh1213 / 測定日: 2008年12月2日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 120 sec. / フレーム数: 1 / 単位: 1/nm /
| ||||||||||||||||||||||||||||||
距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.6 / ポイント数: 400 /
| ||||||||||||||||||||||||||||||
結果 | カーブのタイプ: merged / Standard: BSA /
|