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- PDB-9yh7: Composite structure of the sheathed flagellar motor in Vibrio cho... -

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Basic information

Entry
Database: PDB / ID: 9yh7
TitleComposite structure of the sheathed flagellar motor in Vibrio cholerae adopting a higher FOMC conformation
Components
  • (Flagellar basal-body rod protein ...) x 2
  • (FlgP) x 2
  • (Sodium-type flagellar protein ...) x 2
  • Chemotaxis protein PomB
  • Flagellar L-ring protein
  • Flagellar M-ring protein
  • Flagellar P-ring protein
  • Flagellar hook protein FlgE
  • Flagellar protein FlgT
  • FlgO domain-containing protein
KeywordsMOTOR PROTEIN / Sheathed flagellar motor / Vibrio cholerae
Function / homology
Function and homology information


bacterial-type flagellum basal body, distal rod, L ring / bacterial-type flagellum stator complex / bacterial-type flagellum basal body, distal rod, P ring / bacterial-type flagellum basal body, distal rod / bacterial-type flagellum basal body, rod / bacterial-type flagellum hook / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / bacterial-type flagellum ...bacterial-type flagellum basal body, distal rod, L ring / bacterial-type flagellum stator complex / bacterial-type flagellum basal body, distal rod, P ring / bacterial-type flagellum basal body, distal rod / bacterial-type flagellum basal body, rod / bacterial-type flagellum hook / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / bacterial-type flagellum / cytoskeletal motor activity / bacterial-type flagellum-dependent cell motility / cell outer membrane / outer membrane-bounded periplasmic space / structural molecule activity / plasma membrane / cytosol
Similarity search - Function
Flagellar protein FlgO / FlgO domain / FlgO protein / Flagellar basal-body rod FlgF / Flagellar assembly protein T, N-terminal / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, C-terminal / FlgT, C-terminal domain superfamily / Flagellar assembly protein T, C-terminal domain / Flagellar assembly protein T, middle domain ...Flagellar protein FlgO / FlgO domain / FlgO protein / Flagellar basal-body rod FlgF / Flagellar assembly protein T, N-terminal / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, C-terminal / FlgT, C-terminal domain superfamily / Flagellar assembly protein T, C-terminal domain / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, N-terminal domain / FlgT, N-terminal domain superfamily / MotY N-terminal domain / MotY N-terminal domain / Flagellar protein FlgP / Flagellar L-ring protein / Flagellar L-ring protein / Flagellar P-ring protein / Flagellar P-ring protein / Flagellar basal-body rod FlgG / : / Motility protein B-like, N-terminal domain / Membrane MotB of proton-channel complex MotA/MotB / Sel1 repeat / Flagellar hook protein FlgE superfamily / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Sel1-like repeat / Sel1-like repeats. / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / : / Flagellar hook protein FlgE/F/G D1 domain / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal / Flagellar basal-body/hook protein, C-terminal domain / Flagella basal body rod protein / Flagellar basal body rod FlgEFG protein C-terminal / Flagellar M-ring protein FliF / Flagellar M-ring C-terminal / Flagellar M-ring protein C-terminal / Lipoprotein YscJ/Flagellar M-ring protein / Secretory protein of YscJ/FliF family / Flagellar M-ring , N-terminal / : / OmpA-like domain profile. / OmpA family / OmpA-like domain / OmpA-like domain superfamily / AMP-binding enzyme, C-terminal domain superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Flagellar hook protein FlgE / Sodium-type flagellar protein MotX / Flagellar protein FlgT / FlgO domain-containing protein / Lipoprotein / Flagellar basal-body rod protein FlgF / Flagellar basal-body rod protein FlgG / Flagellar L-ring protein / Flagellar P-ring protein / Flagellar M-ring protein ...Flagellar hook protein FlgE / Sodium-type flagellar protein MotX / Flagellar protein FlgT / FlgO domain-containing protein / Lipoprotein / Flagellar basal-body rod protein FlgF / Flagellar basal-body rod protein FlgG / Flagellar L-ring protein / Flagellar P-ring protein / Flagellar M-ring protein / Sodium-type flagellar protein MotY / Chemotaxis protein PomB
Similarity search - Component
Biological speciesVibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsGuo, W.B. / Yue, J.
Funding support United States, 5items
OrganizationGrant numberCountry
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)R01AI189907 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI087946 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI132818 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI132818 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R37AI102584 United States
CitationJournal: To Be Published
Title: Composite structure of the sheathed flagellar motor in Vibrio cholerae adopting a higher FOMC conformation
Authors: Guo, W.B. / Yue, J.
History
DepositionSep 30, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 10, 2026Provider: repository / Type: Initial release
Revision 1.0Jun 10, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Jun 10, 2026Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
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Revision 1.0Jun 10, 2026Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Jun 10, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
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Revision 1.0Jun 10, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Jun 10, 2026Data content type: Mask / Part number: 1 / Data content type: Mask / Provider: repository / Type: Initial release
Revision 1.0Jun 10, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
Aa: Flagellar basal-body rod protein FlgG
Ab: Flagellar basal-body rod protein FlgG
Ac: Flagellar basal-body rod protein FlgG
Ad: Flagellar basal-body rod protein FlgG
Ae: Flagellar basal-body rod protein FlgG
Af: Flagellar basal-body rod protein FlgG
Ag: Flagellar basal-body rod protein FlgG
Ah: Flagellar basal-body rod protein FlgG
Ai: Flagellar basal-body rod protein FlgG
Aj: Flagellar basal-body rod protein FlgG
Ak: Flagellar basal-body rod protein FlgG
Al: Flagellar basal-body rod protein FlgG
Am: Flagellar basal-body rod protein FlgG
An: Flagellar basal-body rod protein FlgG
Ao: Flagellar basal-body rod protein FlgG
Ap: Flagellar basal-body rod protein FlgG
Aq: Flagellar basal-body rod protein FlgG
Ar: Flagellar basal-body rod protein FlgG
As: Flagellar basal-body rod protein FlgG
At: Flagellar basal-body rod protein FlgG
Au: Flagellar basal-body rod protein FlgG
Av: Flagellar basal-body rod protein FlgG
Aw: Flagellar basal-body rod protein FlgG
Ax: Flagellar basal-body rod protein FlgG
Ay: Flagellar basal-body rod protein FlgG
Az: Flagellar basal-body rod protein FlgG
Ba: Flagellar basal-body rod protein FlgF
Bb: Flagellar basal-body rod protein FlgG
Bc: Flagellar basal-body rod protein FlgF
Bd: Flagellar basal-body rod protein FlgF
Be: Flagellar hook protein FlgE
Bf: Flagellar hook protein FlgE
Bg: Flagellar hook protein FlgE
Bh: Flagellar hook protein FlgE
Bi: Flagellar hook protein FlgE
Bj: Flagellar hook protein FlgE
Bk: Flagellar hook protein FlgE
Bl: Flagellar hook protein FlgE
Bm: Flagellar hook protein FlgE
Bn: Flagellar hook protein FlgE
Bo: Flagellar hook protein FlgE
Bp: Flagellar hook protein FlgE
Bq: Flagellar hook protein FlgE
Br: Flagellar hook protein FlgE
Bs: Flagellar hook protein FlgE
Bt: Flagellar hook protein FlgE
Bu: Flagellar hook protein FlgE
Bv: Flagellar basal-body rod protein FlgF
Bw: Flagellar basal-body rod protein FlgF
Bx: Flagellar L-ring protein
By: Flagellar L-ring protein
Bz: Flagellar L-ring protein
Ca: Flagellar L-ring protein
Cb: Flagellar L-ring protein
Cc: Flagellar L-ring protein
Cd: Flagellar L-ring protein
Ce: Flagellar L-ring protein
Cf: Flagellar L-ring protein
Cg: Flagellar L-ring protein
Ch: Flagellar L-ring protein
Ci: Flagellar L-ring protein
Cj: Flagellar L-ring protein
Ck: Flagellar L-ring protein
Cl: Flagellar L-ring protein
Cm: Flagellar L-ring protein
Cn: Flagellar L-ring protein
Co: Flagellar L-ring protein
Cp: Flagellar L-ring protein
Cq: Flagellar L-ring protein
Cr: Flagellar L-ring protein
Cs: Flagellar L-ring protein
Ct: Flagellar L-ring protein
Cu: Flagellar L-ring protein
Cv: Flagellar L-ring protein
Cw: Flagellar L-ring protein
Cx: Flagellar P-ring protein
Cy: Flagellar P-ring protein
Cz: Flagellar P-ring protein
Da: Flagellar P-ring protein
Db: Flagellar P-ring protein
Dc: Flagellar P-ring protein
Dd: Flagellar P-ring protein
De: Flagellar P-ring protein
Df: Flagellar P-ring protein
Dg: Flagellar P-ring protein
Dh: Flagellar P-ring protein
Di: Flagellar P-ring protein
Dj: Flagellar P-ring protein
Dk: Flagellar P-ring protein
Dl: Flagellar P-ring protein
Dm: Flagellar P-ring protein
Dn: Flagellar P-ring protein
Do: Flagellar P-ring protein
Dp: Flagellar P-ring protein
Dq: Flagellar P-ring protein
Dr: Flagellar P-ring protein
Ds: Flagellar P-ring protein
Dt: Flagellar P-ring protein
Du: Flagellar P-ring protein
Dv: Flagellar P-ring protein
Dw: Flagellar P-ring protein
Dx: Flagellar protein FlgT
Dy: Flagellar protein FlgT
Dz: Flagellar protein FlgT
Ea: Flagellar protein FlgT
Eb: Flagellar protein FlgT
Ec: Flagellar protein FlgT
Ed: Flagellar protein FlgT
Ee: Flagellar protein FlgT
Ef: Flagellar protein FlgT
Eg: Flagellar protein FlgT
Eh: Flagellar protein FlgT
Ei: Flagellar protein FlgT
Ej: Flagellar protein FlgT
Ek: Flagellar protein FlgT
El: Flagellar protein FlgT
Em: Flagellar protein FlgT
En: Flagellar protein FlgT
Eo: Flagellar protein FlgT
Ep: Flagellar protein FlgT
Eq: Flagellar protein FlgT
Er: Flagellar protein FlgT
Es: Flagellar protein FlgT
Et: Flagellar protein FlgT
Eu: Flagellar protein FlgT
Ev: Flagellar protein FlgT
Ew: Flagellar protein FlgT
Ex: Sodium-type flagellar protein MotY
Ey: Sodium-type flagellar protein MotY
Ez: Sodium-type flagellar protein MotY
Fa: Sodium-type flagellar protein MotY
Fb: Sodium-type flagellar protein MotY
Fc: Sodium-type flagellar protein MotY
Fd: Sodium-type flagellar protein MotY
Fe: Sodium-type flagellar protein MotY
Ff: Sodium-type flagellar protein MotY
Fg: Sodium-type flagellar protein MotY
Fh: Sodium-type flagellar protein MotY
Fi: Sodium-type flagellar protein MotY
Fj: Sodium-type flagellar protein MotY
Fk: Sodium-type flagellar protein MotY
Fl: Sodium-type flagellar protein MotY
Fm: Sodium-type flagellar protein MotY
Fn: Sodium-type flagellar protein MotY
Fo: Sodium-type flagellar protein MotY
Fp: Sodium-type flagellar protein MotY
Fq: Sodium-type flagellar protein MotY
Fr: Sodium-type flagellar protein MotY
Fs: Sodium-type flagellar protein MotY
Ft: Sodium-type flagellar protein MotY
Fu: Sodium-type flagellar protein MotY
Fv: Sodium-type flagellar protein MotY
Fw: Sodium-type flagellar protein MotY
Fx: Sodium-type flagellar protein MotX
Fy: Sodium-type flagellar protein MotX
Fz: Sodium-type flagellar protein MotX
Ga: Sodium-type flagellar protein MotX
Gb: Sodium-type flagellar protein MotX
Gc: Sodium-type flagellar protein MotX
Gd: Sodium-type flagellar protein MotX
Ge: Sodium-type flagellar protein MotX
Gf: Sodium-type flagellar protein MotX
Gg: Sodium-type flagellar protein MotX
Gh: Sodium-type flagellar protein MotX
Gi: Sodium-type flagellar protein MotX
Gj: Sodium-type flagellar protein MotX
Gk: Sodium-type flagellar protein MotX
Gl: Sodium-type flagellar protein MotX
Gm: Sodium-type flagellar protein MotX
Gn: Sodium-type flagellar protein MotX
Go: Sodium-type flagellar protein MotX
Gp: Sodium-type flagellar protein MotX
Gq: Sodium-type flagellar protein MotX
Gr: Sodium-type flagellar protein MotX
Gs: Sodium-type flagellar protein MotX
Gt: Sodium-type flagellar protein MotX
Gu: Sodium-type flagellar protein MotX
Gv: Sodium-type flagellar protein MotX
Gw: Sodium-type flagellar protein MotX
Gx: FlgP
Gy: FlgP
Gz: FlgP
Ha: FlgP
Hb: FlgP
Hc: FlgP
Hd: FlgP
He: FlgP
Hf: FlgP
Hg: FlgP
Hh: FlgP
Hi: FlgP
Hj: FlgP
Hk: FlgP
Hl: FlgP
Hm: FlgP
Hn: FlgP
Ho: FlgP
Hp: FlgP
Hq: FlgP
Hr: FlgP
Hs: FlgP
Ht: FlgP
Hu: FlgP
Hv: FlgP
Hw: FlgP
Hx: FlgP
Hy: FlgP
Hz: FlgP
Ia: FlgP
Ib: FlgP
Ic: FlgP
Id: FlgP
Ie: FlgP
If: FlgP
Ig: FlgP
Ih: FlgP
Ii: FlgP
Ij: FlgP
Ik: FlgP
Il: FlgP
Im: FlgP
In: FlgP
Io: FlgP
Ip: FlgP
Iq: FlgP
Ir: FlgP
Is: FlgP
It: FlgP
Iu: FlgP
Iv: FlgP
Iw: FlgP
Ix: Chemotaxis protein PomB
Iy: Chemotaxis protein PomB
Iz: Chemotaxis protein PomB
Ja: Chemotaxis protein PomB
Jb: Chemotaxis protein PomB
Jc: Chemotaxis protein PomB
Jd: Chemotaxis protein PomB
Je: Chemotaxis protein PomB
Jf: Chemotaxis protein PomB
Jg: Chemotaxis protein PomB
Jh: Chemotaxis protein PomB
Ji: Chemotaxis protein PomB
Jj: Chemotaxis protein PomB
Jk: Chemotaxis protein PomB
Jl: Chemotaxis protein PomB
Jm: Chemotaxis protein PomB
Jn: Chemotaxis protein PomB
Jo: Chemotaxis protein PomB
Jp: Chemotaxis protein PomB
Jq: Chemotaxis protein PomB
Jr: Chemotaxis protein PomB
Js: Chemotaxis protein PomB
Jt: Chemotaxis protein PomB
Ju: Chemotaxis protein PomB
Jv: Chemotaxis protein PomB
Jw: Chemotaxis protein PomB
Jx: FlgP
Jy: FlgP
Jz: FlgP
Ka: FlgP
Kb: FlgP
Kc: FlgP
Kd: FlgP
Ke: FlgP
Kf: FlgP
Kg: FlgP
Kh: FlgP
Ki: FlgP
Kj: FlgP
Kk: FlgP
Kl: FlgP
Km: FlgP
Kn: FlgP
Ko: FlgP
Kp: FlgP
Kq: FlgP
Kr: FlgP
Ks: FlgP
Kt: FlgP
Ku: FlgP
Kv: FlgP
Kw: FlgP
Kx: FlgP
Ky: FlgP
Kz: FlgP
La: FlgP
Lb: FlgP
Lc: FlgP
Ld: FlgP
Le: FlgP
Lf: FlgP
Lg: FlgP
Lh: FlgP
Li: FlgP
Lj: FlgP
Lk: FlgP
Ll: FlgP
Lm: FlgP
Ln: FlgP
Lo: FlgP
Lp: FlgP
Lq: FlgP
Lr: FlgP
Ls: FlgP
Lt: FlgP
Lu: FlgP
Lv: FlgP
Lw: FlgP
Lx: FlgP
Ly: FlgP
Lz: FlgP
Ma: FlgP
Mb: FlgP
Mc: FlgP
Md: FlgO domain-containing protein
Me: FlgO domain-containing protein
Mf: FlgO domain-containing protein
Mg: FlgO domain-containing protein
Mh: FlgO domain-containing protein
Mi: FlgO domain-containing protein
Mj: FlgO domain-containing protein
Mk: FlgO domain-containing protein
Ml: FlgO domain-containing protein
Mm: FlgO domain-containing protein
Mn: FlgO domain-containing protein
Mo: FlgO domain-containing protein
Mp: FlgO domain-containing protein
Mq: FlgO domain-containing protein
Mr: FlgO domain-containing protein
Ms: FlgO domain-containing protein
Mt: FlgO domain-containing protein
Mu: FlgO domain-containing protein
Mv: FlgO domain-containing protein
Mw: FlgO domain-containing protein
Mx: FlgO domain-containing protein
My: FlgO domain-containing protein
Mz: FlgO domain-containing protein
Na: FlgO domain-containing protein
Nb: FlgO domain-containing protein
Nc: FlgO domain-containing protein
Nd: FlgO domain-containing protein
Ne: FlgO domain-containing protein
Nf: FlgO domain-containing protein
Ng: FlgO domain-containing protein
Nh: FlgO domain-containing protein
Ni: FlgO domain-containing protein
Nj: FlgO domain-containing protein
Nk: FlgO domain-containing protein
Nl: FlgO domain-containing protein
Nm: FlgO domain-containing protein
Nn: FlgO domain-containing protein
No: FlgO domain-containing protein
Np: FlgO domain-containing protein
Nq: FlgO domain-containing protein
Nr: FlgO domain-containing protein
Ns: FlgO domain-containing protein
Nt: FlgO domain-containing protein
Nu: FlgO domain-containing protein
Nv: FlgO domain-containing protein
Nw: FlgO domain-containing protein
Nx: FlgO domain-containing protein
Ny: FlgO domain-containing protein
Nz: FlgO domain-containing protein
Oa: FlgO domain-containing protein
Ob: FlgO domain-containing protein
Oc: FlgO domain-containing protein
Od: FlgO domain-containing protein
Oe: FlgO domain-containing protein
Of: FlgO domain-containing protein
Og: FlgO domain-containing protein
Oh: FlgO domain-containing protein
Oi: FlgO domain-containing protein
Oj: Flagellar M-ring protein
Ok: Flagellar M-ring protein
Ol: Flagellar M-ring protein
Om: Flagellar M-ring protein
On: Flagellar M-ring protein
Oo: Flagellar M-ring protein
Op: Flagellar M-ring protein
Oq: Flagellar M-ring protein
Or: Flagellar M-ring protein
Os: Flagellar M-ring protein
Ot: Flagellar M-ring protein
Ou: Flagellar M-ring protein
Ov: Flagellar M-ring protein
Ow: Flagellar M-ring protein
Ox: Flagellar M-ring protein
Oy: Flagellar M-ring protein
Oz: Flagellar M-ring protein
Pa: Flagellar M-ring protein
Pb: Flagellar M-ring protein
Pc: Flagellar M-ring protein
Pd: Flagellar M-ring protein
Pe: Flagellar M-ring protein
Pf: Flagellar M-ring protein
Pg: Flagellar M-ring protein
Ph: Flagellar M-ring protein
Pi: Flagellar M-ring protein
Pj: Flagellar M-ring protein
Pk: Flagellar M-ring protein
Pl: Flagellar M-ring protein
Pm: Flagellar M-ring protein
Pn: Flagellar M-ring protein
Po: Flagellar M-ring protein
Pp: Flagellar M-ring protein
Pq: Flagellar M-ring protein


Theoretical massNumber of molelcules
Total (without water)8,841,086407
Polymers8,841,086407
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Flagellar basal-body rod protein ... , 2 types, 32 molecules AaAbAcAdAeAfAgAhAiAjAkAlAmAnAoApAqArAsAtAuAvAwAxAyAzBbBaBcBdBvBw

#1: Protein ...
Flagellar basal-body rod protein FlgG / Distal rod protein


Mass: 28114.350 Da / Num. of mol.: 27
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2195
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ12
#2: Protein
Flagellar basal-body rod protein FlgF


Mass: 26778.270 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2196
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ11

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Protein , 8 types, 271 molecules BeBfBgBhBiBjBkBlBmBnBoBpBqBrBsBtBuBxByBzCaCbCcCdCeCfCgChCiCj...

#3: Protein
Flagellar hook protein FlgE


Mass: 46880.289 Da / Num. of mol.: 17
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: flgE, D6U24_04930, ERS013165_00512, ERS013200_03135, EYB64_08495, F0M16_14475, KIN13_20260
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: A0A085QTL5
#4: Protein ...
Flagellar L-ring protein / Basal body L-ring protein


Mass: 24256.520 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: flgH, VC_2194
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ13
#5: Protein ...
Flagellar P-ring protein / Basal body P-ring protein


Mass: 35702.562 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: flgI, VC_2193
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ14
#6: Protein ...
Flagellar protein FlgT


Mass: 39808.223 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2208
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KPZ9
#10: Protein ...
Chemotaxis protein PomB


Mass: 17542.775 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_0893
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KTK9
#11: Protein ...
FlgP / Lipoprotein


Mass: 11848.299 Da / Num. of mol.: 58
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2206
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ01
#12: Protein ...
FlgO domain-containing protein


Mass: 21395.104 Da / Num. of mol.: 58
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2207
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ00
#13: Protein ...
Flagellar M-ring protein


Mass: 21495.785 Da / Num. of mol.: 34
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2133
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ69

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Sodium-type flagellar protein ... , 2 types, 52 molecules ExEyEzFaFbFcFdFeFfFgFhFiFjFkFlFmFnFoFpFqFrFsFtFuFvFwFxFyFzGa...

#7: Protein ...
Sodium-type flagellar protein MotY


Mass: 31207.955 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_1008
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KT95
#8: Protein ...
Sodium-type flagellar protein MotX


Mass: 21244.137 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2601
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KNX9

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Protein/peptide , 1 types, 52 molecules GxGyGzHaHbHcHdHeHfHgHhHiHjHkHlHmHnHoHpHqHrHsHtHuHvHwHxHyHzIa...

#9: Protein/peptide ...
FlgP / Lipoprotein


Mass: 1506.766 Da / Num. of mol.: 52
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Gene: VC_2206
Production host: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
References: UniProt: Q9KQ01

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: CELL / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Vibrio cholerae DflhG / Type: CELL / Entity ID: all / Source: NATURAL
Source (natural)Organism: Vibrio cholerae O1 biovar El Tor str. N16961 (bacteria)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 1600 nm / Cs: 2.7 mm / C2 aperture diameter: 100 µm
Image recordingElectron dose: 70 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameVersionCategory
1Topazparticle selection
2PHENIX1.21.2_5419model refinement
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C26 (26 fold cyclic)
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 5068 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 150.29 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0017583596
ELECTRON MICROSCOPYf_angle_d0.4146789390
ELECTRON MICROSCOPYf_chiral_restr0.041189430
ELECTRON MICROSCOPYf_plane_restr0.0032104331
ELECTRON MICROSCOPYf_dihedral_angle_d10.5191218100

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