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- PDB-9sii: Supercoiling bacterial archaellum filament from L. aerophila -

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Basic information

Entry
Database: PDB / ID: 9sii
TitleSupercoiling bacterial archaellum filament from L. aerophila
ComponentsFlagellin
KeywordsPROTEIN FIBRIL / Helical / cell surface structure / motility / archaellum / Supercoiling / Superhelicity
Function / homologyFlagellin, archaea / Archaeal-type flagellin / Flagellin/pilin, N-terminal / archaeal or bacterial-type flagellum-dependent cell motility / structural molecule activity / membrane / Flagellin
Function and homology information
Biological speciesLitorilinea aerophila (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsSivabalasarma, S. / Taib, N. / Mollat, C.L. / Joest, M. / Steimle, S. / Gribaldo, S. / Albers, S.-V.
Funding support Germany, 1items
OrganizationGrant numberCountry
German Research Foundation (DFG)403222702 Germany
Citation
Journal: Nat Microbiol / Year: 2025
Title: Structure of a functional archaellum in Bacteria of the Chloroflexota phylum.
Authors: Shamphavi Sivabalasarma / Najwa Taib / Clara L Mollat / Marie Joest / Stefan Steimle / Simonetta Gribaldo / Sonja-Verena Albers /
Abstract: Motility in Archaea is driven by the archaellum, a rotary ATP-driven machinery unrelated to the bacterial flagellum. To date, archaella have been described exclusively in archaea; however, recent ...Motility in Archaea is driven by the archaellum, a rotary ATP-driven machinery unrelated to the bacterial flagellum. To date, archaella have been described exclusively in archaea; however, recent work reported archaellum genes in bacterial strains of the SAR202 clade (Chloroflexota). Here, using MacSyFinder, we show that bona fide archaellum gene clusters are widespread in several members of the Chloroflexota. Analysis of archaellum-encoding loci and Alphafold3-predicted structures show similarity to the archaellum machinery. Using cryo electron microscopy single-particle analysis, we solved the structure of the bacterial archaellum from Litorilinea aerophila to 2.7 Å. We also show the expression and assembly of this machinery in bacteria and its function in swimming motility. Finally, a phylogenomic analysis revealed two horizontal gene transfer events from euryarchaeal members to Chloroflexota. In summary, our study shows that a functional and assembled archaellum machinery can be exchanged between the two prokaryotic domains.
#1: Journal: To Be Published
Title: Horizontal gene transfer of the functional archaellum machinery to Bacteria
Authors: Sivabalasarma, S. / Taib, N. / Mollat, C.L. / Joest, M. / Steimle, S. / Gribaldo, S. / Albers, S.-V.
History
DepositionAug 28, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jan 28, 2026Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A0: Flagellin
A1: Flagellin
A3: Flagellin
A4: Flagellin
A5: Flagellin
A6: Flagellin
A7: Flagellin
A8: Flagellin
A9: Flagellin
Ab: Flagellin
Ac: Flagellin
Ad: Flagellin
Ae: Flagellin
Af: Flagellin
Ag: Flagellin
Ai: Flagellin
Aj: Flagellin
Ak: Flagellin
Al: Flagellin
Am: Flagellin
An: Flagellin
Ao: Flagellin
Ap: Flagellin
Aq: Flagellin
Ar: Flagellin
At: Flagellin
Au: Flagellin
Ay: Flagellin
Az: Flagellin
B: Flagellin
E: Flagellin
F: Flagellin
G: Flagellin
H: Flagellin
I: Flagellin
J: Flagellin
K: Flagellin
L: Flagellin
M: Flagellin
N: Flagellin
O: Flagellin
P: Flagellin
R: Flagellin
S: Flagellin
T: Flagellin
U: Flagellin
V: Flagellin
W: Flagellin
X: Flagellin
Y: Flagellin
Z: Flagellin
Av: Flagellin


Theoretical massNumber of molelcules
Total (without water)1,012,37052
Polymers1,012,37052
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "Ab"
d_2ens_1chain "A1"
d_3ens_1chain "A3"
d_4ens_1chain "A4"
d_5ens_1chain "A5"
d_6ens_1chain "A6"
d_7ens_1chain "A7"
d_8ens_1chain "A8"
d_9ens_1chain "A9"
d_10ens_1chain "A0"
d_11ens_1chain "Ac"
d_12ens_1chain "Ad"
d_13ens_1chain "Ae"
d_14ens_1chain "Af"
d_15ens_1chain "Ag"
d_16ens_1chain "Ai"
d_17ens_1chain "Aj"
d_18ens_1chain "Ak"
d_19ens_1chain "Al"
d_20ens_1chain "Am"
d_21ens_1chain "An"
d_22ens_1chain "Ao"
d_23ens_1chain "Ap"
d_24ens_1chain "Aq"
d_25ens_1chain "Ar"
d_26ens_1chain "At"
d_27ens_1chain "Au"
d_28ens_1chain "Av"
d_29ens_1chain "Ay"
d_30ens_1chain "Az"
d_31ens_1chain "B"
d_32ens_1chain "E"
d_33ens_1chain "F"
d_34ens_1chain "G"
d_35ens_1chain "H"
d_36ens_1chain "I"
d_37ens_1chain "J"
d_38ens_1chain "K"
d_39ens_1chain "L"
d_40ens_1chain "M"
d_41ens_1chain "N"
d_42ens_1chain "O"
d_43ens_1chain "P"
d_44ens_1chain "R"
d_45ens_1chain "S"
d_46ens_1chain "T"
d_47ens_1chain "U"
d_48ens_1chain "V"
d_49ens_1chain "W"
d_50ens_1chain "X"
d_51ens_1chain "Y"
d_52ens_1chain "Z"

NCS domain segments:

Component-ID: 1 / Ens-ID: ens_1 / Beg auth comp-ID: ILE / Beg label comp-ID: ILE / End auth comp-ID: GLN / End label comp-ID: GLN / Auth seq-ID: 1 - 183 / Label seq-ID: 1 - 183

Dom-IDAuth asym-IDLabel asym-ID
d_1AbJ
d_2A1B
d_3A3C
d_4A4D
d_5A5E
d_6A6F
d_7A7G
d_8A8H
d_9A9I
d_10A0A
d_11AcK
d_12AdL
d_13AeM
d_14AfN
d_15AgO
d_16AiP
d_17AjQ
d_18AkR
d_19AlS
d_20AmT
d_21AnU
d_22AoV
d_23ApW
d_24AqX
d_25ArY
d_26AtZ
d_27AuAA
d_28AvZA
d_29AyBA
d_30AzCA
d_31BDA
d_32EEA
d_33FFA
d_34GGA
d_35HHA
d_36IIA
d_37JJA
d_38KKA
d_39LLA
d_40MMA
d_41NNA
d_42OOA
d_43PPA
d_44RQA
d_45SRA
d_46TSA
d_47UTA
d_48VUA
d_49WVA
d_50XWA
d_51YXA
d_52ZYA

NCS oper:
IDCodeMatrixVector
1given(-0.261435851692, 0.964840285926, 0.0271019944506), (-0.965151920704, -0.260978541418, -0.0192865466187), (-0.0115353981729, -0.0311997367404, 0.999446602384)8.35938230523, 122.29672904, 108.01285107
2given(0.775650614463, -0.631082666679, -0.0100395268969), (0.631097118826, 0.77524314561, 0.026729979335), (-0.00908575222705, -0.0270690413947, 0.999592274932)48.2908463137, -26.0509675373, 95.6402198506
3given(0.747782845634, -0.663925689239, -0.00484715828827), (0.663200869801, 0.746581493356, 0.0527321540803), (-0.0313914330694, -0.0426468398274, 0.998596928186)51.0879039189, -28.9873516201, 207.830993715
4given(-0.236979124508, 0.971012132929, 0.0312463158102), (-0.971511165656, -0.236942924991, -0.00490971513094), (0.00263620050188, -0.0315196446885, 0.999499656051)6.25656319195, 119.451603751, 162.375920864
5given(-0.78089548747, 0.624512655496, 0.0136448075958), (-0.624399021534, -0.779747467113, -0.0460407367227), (-0.0181135185896, -0.0444728080583, 0.998846369462)56.9501658535, 136.999103645, 71.1910881176
6given(0.800466497222, 0.599318725742, 0.00839355707779), (-0.599095688472, 0.800441061057, -0.0194541468139), (-0.0183777822123, 0.0105438489003, 0.999775516989)-23.6604819602, 46.9015226334, -38.0736125432
7given(0.999698127031, -0.0243943739165, 0.00292734211001), (0.0243548442554, 0.999620729172, 0.0128545467078), (-0.00323981047346, -0.0127793713064, 0.999913091872)0.587070720333, -4.40097264307, 55.9497231584
8given(-0.998212926496, 0.0595682666595, -0.00475131380624), (-0.0592655512042, -0.997042584072, -0.0489252489785), (-0.00765165447265, -0.0485562267307, 0.998791141846)102.592689455, 119.40076633, 87.8767588231
9given(0.801058390202, -0.598353178041, -0.0167011920104), (0.598453051697, 0.801156884298, 0.00126160902303), (0.0126253871867, -0.0110055018186, 0.999859729426)46.7107051909, -19.7181370975, -16.3809237325
10given(0.761308549045, -0.648353109293, -0.0068948401971), (0.648040817078, 0.760506977585, 0.0408929877303), (-0.021269521664, -0.03560031903, 0.999139742345)49.5982339074, -28.0725461337, 151.844060975
11given(0.824121665676, 0.566219768672, 0.0147869445342), (-0.566357232884, 0.824125665549, 0.00750813852444), (-0.00793504404725, -0.0145623126161, 0.999862477607)-25.1128129243, 37.1228318534, 73.0171360578
12given(-0.765748405077, 0.642900210791, 0.0175698345776), (-0.643107215921, -0.765146408028, -0.0310496877947), (-0.00651835501153, -0.0350755363063, 0.999363406275)54.4818215797, 134.736935035, 124.960720545
13given(-0.996412837884, 0.0845797516562, 0.00277886859061), (-0.0846252296547, -0.995809837322, -0.0346603288934), (-0.000164337330971, -0.0347711590673, 0.999395286906)100.051487833, 118.547575876, 141.570877585
14given(0.995567090304, -0.0932177446316, 0.0125148227592), (0.0924060693587, 0.994224843982, 0.0545717688444), (-0.0175296049174, -0.0531734115413, 0.998431420407)3.56277963085, -12.7794445092, 224.500930456
15given(0.233878074566, -0.971861632043, -0.0280359483631), (0.972222554074, 0.233497998936, 0.0161861002644), (-0.00918431197828, -0.031042755288, 0.999475860518)98.1867726676, -11.439937409, 79.7316583766
16given(0.851823907902, 0.523253409438, 0.0245336389218), (-0.52382408978, 0.851068930327, 0.035916553262), (-0.00208635888464, -0.0434458898352, 0.999053603048)-25.3202882014, 29.6122092616, 184.776014086
17given(0.388999225085, 0.920582771613, 0.0347413801211), (-0.921201326253, 0.388370138686, 0.0235955904238), (0.00822917941442, -0.0411824718337, 0.99911775313)-24.4573423539, 78.5426242221, 200.947702665
18given(-0.993837501588, 0.11068539116, 0.00598035285511), (-0.110784698893, -0.99363994093, -0.0201598184687), (0.00371092006476, -0.0206981152097, 0.999778884103)98.1844251773, 118.47804202, 195.441793932
19given(-0.842074155942, -0.538266996033, -0.0343475891003), (0.539354226816, -0.840689937951, -0.048347143069), (-0.00285200108301, -0.0592373970543, 0.998239849375)131.635434949, 79.5520990141, 50.1522517219
20given(-0.86794667487, -0.496289353933, -0.0191166617388), (0.496629318556, -0.86766373857, -0.0227806216146), (-0.00528105420997, -0.0292662594742, 0.999557700447)128.428525279, 79.1903961074, 158.195176084
21given(-0.748948765946, 0.662259403294, 0.0220958986521), (-0.662608621158, -0.748764892691, -0.0173479289617), (0.00505580410055, -0.0276336429267, 0.999605332431)52.0277447195, 133.3449559, 178.924652011
22given(-0.999164211631, 0.0396562795804, -0.00991250145838), (-0.0389529517773, -0.997240513671, -0.0631983025123), (-0.0123913575996, -0.0627593609148, 0.997951760795)104.644004048, 121.207491866, 34.1466208859
23given(-0.999780591449, 0.0161058557304, -0.0133929224674), (-0.0151209316194, -0.997386844877, -0.070645871028), (-0.0144957368901, -0.070427857255, 0.997411545218)106.972530151, 122.670607572, -20.2212083009
24given(0.789180352108, -0.614032854267, -0.0125708284394), (0.614158416265, 0.789076100094, 0.012974898524), (0.00195232630537, -0.0179600150712, 0.999836799823)47.1750163555, -23.1916609006, 39.4925811202
25given(0.324206602743, 0.945685346625, 0.023860090457), (-0.945627427182, 0.324675193923, -0.0193594269694), (-0.0260547059002, -0.0162863019024, 0.999527842869)-20.8571509494, 89.5112578993, 35.8559211394
26given(0.346633376646, 0.937600210561, 0.0274070675384), (-0.937895081169, 0.34688481705, -0.00487241406509), (-0.0140754720623, -0.0240160124937, 0.99961248103)-22.3264330958, 84.9721629908, 90.7575371518
27given(0.258971974857, -0.965357635046, -0.0319085051033), (0.965853509295, 0.259088291994, 0.000505504642225), (0.00777912732135, -0.0309498531658, 0.999490666173)97.3352475239, -9.42136453944, 23.9911554139
28given(0.998915602167, -0.0462214996908, 0.00558504371995), (0.0460543763212, 0.998572572213, 0.0270520321888), (-0.0068274569708, -0.0267654813185, 0.999618424621)1.27372274851, -8.04375106074, 112.068354286
29given(-0.282855304577, 0.958901051856, 0.0223975316165), (-0.958804977817, -0.282035153568, -0.0338996558322), (-0.026189524366, -0.0310635622802, 0.999174241017)10.6677049225, 125.753551482, 53.713774664
30given(0.366841082436, 0.929783704829, 0.0304939743675), (-0.930282277591, 0.366699177544, 0.0103245914677), (-0.00158247841489, -0.0321554882371, 0.999481625813)-23.4444744977, 81.3277520961, 145.736358278
31given(0.809677045984, 0.586710031843, 0.0139434479903), (-0.586700367873, 0.809787379079, -0.0052037508215), (-0.0143443210136, -0.00396726847213, 0.999889244484)-25.076443923, 41.8684471775, 17.6988752366
32given(-0.335335916004, -0.940380251605, -0.0568753534483), (0.942015724818, -0.33389393062, -0.0334845828663), (0.0124979051424, -0.0648060605704, 0.997819611393)131.085365967, 31.304960817, -43.7126305491
33given(-0.385456328928, -0.921380860827, -0.0498069050532), (0.922720741738, -0.385075762409, -0.0174094793416), (-0.00313867087425, -0.05266845837, 0.998607121063)130.547167764, 29.8828842036, 66.2431449156
34given(-0.365205462583, -0.929420452539, -0.0529395173923), (0.930896683201, -0.364144111563, -0.0288172035352), (0.00750568482327, -0.0598054212982, 0.998181835278)130.658545388, 31.1644088437, 11.2433089368
35given(0.195087777671, -0.980555215617, -0.0212656561225), (0.980017071881, 0.194030764083, 0.0438018425473), (-0.0388239336572, -0.0293859101653, 0.998813881791)100.104980463, -13.3312921756, 190.81146138
36given(-0.810246626572, 0.586077339668, 0.00370891573418), (-0.5848803183, -0.808153153285, -0.0693072442213), (-0.0376220333671, -0.0583252326425, 0.997588467176)62.965655746, 142.898673132, -36.79027491
37given(0.283640465517, -0.958444016195, -0.0305475717883), (0.958798856814, 0.283985763559, -0.00753911587346), (0.0159008959955, -0.0271505785731, 0.999504881224)95.9132563839, -7.83122894824, -31.4920524852
38given(-0.827725586104, -0.55982712541, -0.0382615180579), (0.561132427819, -0.825692748161, -0.0579817564879), (0.00086750206685, -0.0694627618922, 0.997584167953)133.095250138, 80.3587915269, -4.31074998687
39given(0.999641804621, 0.0260612147906, -0.0060889685566), (-0.0261057091777, 0.999632197366, -0.00734587898447), (0.00589528648789, 0.00750220456689, 0.999954480226)-0.194397594989, 4.47829326822, -55.5360293277
40given(-0.431483301243, -0.901196217258, -0.0408355084274), (0.901760640474, -0.432148057161, 0.00870654828132), (-0.0254932940066, -0.0330671240387, 0.999127948397)130.611393953, 29.9953081781, 176.050353858
41given(-0.214145725307, 0.97616031962, 0.0353926366947), (-0.976668290079, -0.214574751387, 0.00875940765066), (0.0161449523916, -0.0326910762574, 0.999335095974)4.47785402681, 117.53761213, 216.892115253
42given(-0.406779720567, -0.912639874114, -0.0402333084932), (0.91346309079, -0.406874901926, -0.00616408530974), (-0.0107443334062, -0.0392590672287, 0.999171299097)129.774426375, 29.5676307247, 120.674150933
43given(-0.795335706204, 0.606100989034, 0.00909425804781), (-0.605470065717, -0.793608604928, -0.059928137839), (-0.029105222173, -0.0531692888475, 0.998161265911)59.6677063724, 139.912712465, 17.3887471
44given(-0.856327372763, -0.515625501043, -0.0288751334189), (0.516392473126, -0.855623763522, -0.0353098993989), (-0.0064995657587, -0.0451477349428, 0.998959177181)130.107272826, 79.0931374676, 104.351424513
45given(-0.333206631162, 0.942810362065, 0.00905329397914), (-0.941662128069, -0.332287524752, -0.0534550040124), (-0.04738963504, -0.0263367058802, 0.998529218608)17.2158224328, 134.128013913, -55.4762570671
46given(-0.877745649995, -0.478805984904, -0.0175329043405), (0.479004648041, -0.877760531215, -0.00953923454774), (-0.0108222488348, -0.0167713643013, 0.999800780291)128.141454908, 79.8269018923, 212.686656188
47given(0.838354237322, 0.544729562997, 0.0207816255437), (-0.545099580331, 0.838078598328, 0.0221519874597), (-0.00534979315819, -0.0298992679144, 0.999538600301)-25.5126390701, 32.8763523066, 128.975496122
48given(0.216067245505, -0.976056240256, -0.0250830874881), (0.976051499824, 0.215258790566, 0.0314185100733), (-0.0252668777402, -0.0312708960924, 0.999191531162)99.0073141851, -13.1546355532, 135.48274572
49given(0.997574755014, -0.0689904965757, 0.00921517989223), (0.0685607156076, 0.996811775943, 0.040813130434), (-0.0120015179695, -0.0400823492662, 0.999124300997)2.18599792941, -10.7768764042, 168.243509973
50given(0.300553597128, 0.953561442305, 0.0197005330697), (-0.953078086972, 0.301056836629, -0.0317323376625), (-0.036189713835, -0.00923887814061, 0.99930223043)-18.9940710919, 94.6504853139, -19.0381174471
51given(-0.306315706792, 0.95176272321, 0.0178439479844), (-0.951082047233, -0.305198071759, -0.0479278251787), (-0.0401699788922, -0.0316521042243, 0.998691402333)13.3580706181, 130.047736771, -0.501689959107

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Components

#1: Protein ...
Flagellin


Mass: 19468.656 Da / Num. of mol.: 52 / Source method: isolated from a natural source / Source: (natural) Litorilinea aerophila (bacteria) / References: UniProt: A0A540VDN8
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: supercoiling archaellum filament of L. aerophila / Type: ORGANELLE OR CELLULAR COMPONENT
Details: Archaellum filament isolated from the cell surface of L. aerophila
Entity ID: all / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Litorilinea aerophila (bacteria) / Strain: DSM:25763 / Cellular location: cell surface
Buffer solutionpH: 7.4 / Details: 2% NaCl in 1x PBS, pH 7.4
Buffer component
IDConc.NameFormulaBuffer-ID
12 %sodium chlorideNaCl1
21 xphosphate buffered salinePBS1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: sheared and purified archaella filament from L. aerophila
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 295.15 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Calibrated magnification: 130000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 500 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)
EM imaging opticsEnergyfilter name: TFS Selectris / Energyfilter slit width: 10 eV

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Processing

EM software
IDNameVersionCategory
1cryoSPARCparticle selection
2PHENIX1.21.2_5419model refinement
13cryoSPARC3D reconstruction
CTF correctionType: NONE
3D reconstructionResolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 130246 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 153.51 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.009372436
ELECTRON MICROSCOPYf_angle_d0.950699320
ELECTRON MICROSCOPYf_chiral_restr0.053112740
ELECTRON MICROSCOPYf_plane_restr0.003912636
ELECTRON MICROSCOPYf_dihedral_angle_d19.246325012
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2JAbELECTRON MICROSCOPYNCS constraints0.000703753713333
ens_1d_3JAbELECTRON MICROSCOPYNCS constraints0.000714326420558
ens_1d_4JAbELECTRON MICROSCOPYNCS constraints0.000721661642666
ens_1d_5JAbELECTRON MICROSCOPYNCS constraints0.000702404815763
ens_1d_6JAbELECTRON MICROSCOPYNCS constraints0.000713037507156
ens_1d_7JAbELECTRON MICROSCOPYNCS constraints0.000689382043833
ens_1d_8JAbELECTRON MICROSCOPYNCS constraints0.000715302652264
ens_1d_9JAbELECTRON MICROSCOPYNCS constraints0.000703021031304
ens_1d_10JAbELECTRON MICROSCOPYNCS constraints0.000499008899356
ens_1d_11JAbELECTRON MICROSCOPYNCS constraints0.000708959075276
ens_1d_12JAbELECTRON MICROSCOPYNCS constraints0.000702068436678
ens_1d_13JAbELECTRON MICROSCOPYNCS constraints0.000702714980795
ens_1d_14JAbELECTRON MICROSCOPYNCS constraints0.000711274128634
ens_1d_15JAbELECTRON MICROSCOPYNCS constraints0.00070011395701
ens_1d_16JAbELECTRON MICROSCOPYNCS constraints0.000713091130844
ens_1d_17JAbELECTRON MICROSCOPYNCS constraints0.000698225158369
ens_1d_18JAbELECTRON MICROSCOPYNCS constraints0.000704185718122
ens_1d_19JAbELECTRON MICROSCOPYNCS constraints0.000704545326401
ens_1d_20JAbELECTRON MICROSCOPYNCS constraints0.000703533599712
ens_1d_21JAbELECTRON MICROSCOPYNCS constraints0.000708632843782
ens_1d_22JAbELECTRON MICROSCOPYNCS constraints0.000710201448292
ens_1d_23JAbELECTRON MICROSCOPYNCS constraints0.000700396188509
ens_1d_24JAbELECTRON MICROSCOPYNCS constraints0.000702312837374
ens_1d_25JAbELECTRON MICROSCOPYNCS constraints0.000703477242799
ens_1d_26JAbELECTRON MICROSCOPYNCS constraints0.000708076965281
ens_1d_27JAbELECTRON MICROSCOPYNCS constraints0.000706131167543
ens_1d_28JAbELECTRON MICROSCOPYNCS constraints0.309456905981
ens_1d_29JAbELECTRON MICROSCOPYNCS constraints0.000708387292555
ens_1d_30JAbELECTRON MICROSCOPYNCS constraints0.000704105499206
ens_1d_31JAbELECTRON MICROSCOPYNCS constraints0.000718429497572
ens_1d_32JAbELECTRON MICROSCOPYNCS constraints0.000709581752857
ens_1d_33JAbELECTRON MICROSCOPYNCS constraints0.000713564447871
ens_1d_34JAbELECTRON MICROSCOPYNCS constraints0.141186255826
ens_1d_35JAbELECTRON MICROSCOPYNCS constraints0.190575871597
ens_1d_36JAbELECTRON MICROSCOPYNCS constraints0.142124735008
ens_1d_37JAbELECTRON MICROSCOPYNCS constraints0.000705580975307
ens_1d_38JAbELECTRON MICROSCOPYNCS constraints0.000705847146284
ens_1d_39JAbELECTRON MICROSCOPYNCS constraints0.000696632342423
ens_1d_40JAbELECTRON MICROSCOPYNCS constraints0.000714459423283
ens_1d_41JAbELECTRON MICROSCOPYNCS constraints0.124010128012
ens_1d_42JAbELECTRON MICROSCOPYNCS constraints0.000711777371341
ens_1d_43JAbELECTRON MICROSCOPYNCS constraints0.170657761464
ens_1d_44JAbELECTRON MICROSCOPYNCS constraints0.000696556174972
ens_1d_45JAbELECTRON MICROSCOPYNCS constraints0.000706107129177
ens_1d_46JAbELECTRON MICROSCOPYNCS constraints0.000689053796672
ens_1d_47JAbELECTRON MICROSCOPYNCS constraints0.000696004383869
ens_1d_48JAbELECTRON MICROSCOPYNCS constraints0.000710576611662
ens_1d_49JAbELECTRON MICROSCOPYNCS constraints0.000704757407146
ens_1d_50JAbELECTRON MICROSCOPYNCS constraints0.000698613664774
ens_1d_51JAbELECTRON MICROSCOPYNCS constraints0.000697170612073
ens_1d_52JAbELECTRON MICROSCOPYNCS constraints0.000697591611917

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