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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 9qqb | ||||||||||||
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タイトル | Quaternary complex of a translating ribosome, NAC, NMT1, and NatA | ||||||||||||
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![]() | RIBOSOME / Translation / co-translational protein processing | ||||||||||||
機能・相同性 | ![]() negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / [histone H4]-N-methyl-L-lysine20 N-methyltransferase / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis / N-terminal protein amino acid acetylation ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / [histone H4]-N-methyl-L-lysine20 N-methyltransferase / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis / N-terminal protein amino acid acetylation / myristoyltransferase activity / N-terminal peptidyl-glycine N-myristoylation / peptidyl-lysine N6-myristoyltransferase activity / [histone H4]-lysine20 N-methyltransferase / NatA complex / positive regulation of skeletal muscle tissue growth / protein N-terminal-serine acetyltransferase activity / Late Phase of HIV Life Cycle / protein-N-terminal-alanine acetyltransferase activity / ketone metabolic process / histone H4K20 methyltransferase activity / regulation of opsin-mediated signaling pathway / cardiac ventricle development / protein-N-terminal amino-acid acetyltransferase activity / internal protein amino acid acetylation / Activation, myristolyation of BID and translocation to mitochondria / positive regulation of establishment of protein localization to mitochondrion / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / N-acetyltransferase activity / heart trabecula morphogenesis / skeletal muscle tissue regeneration / acetyltransferase activator activity / S-adenosyl-L-methionine binding / protein localization to membrane / ribosomal subunit / laminin receptor activity / protein acetylation / positive regulation of double-strand break repair via nonhomologous end joining / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / Response of EIF2AK4 (GCN2) to amino acid deficiency / positive regulation of signal transduction by p53 class mediator / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / 90S preribosome / chromosome organization / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / phagocytic cup / pericentric heterochromatin / protein-RNA complex assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / eNOS activation / rough endoplasmic reticulum / laminin binding / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / cellular response to interleukin-4 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / wound healing / spindle / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / rRNA processing / antimicrobial humoral immune response mediated by antimicrobial peptide / rhythmic process / positive regulation of canonical Wnt signaling pathway / Inactivation, recovery and regulation of the phototransduction cascade / protein transport / heparin binding / large ribosomal subunit / regulation of translation / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() ![]() | ||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.43 Å | ||||||||||||
![]() | Echeverria, B. / Jaskolowski, M. / Scaiola, A. / Ban, N. | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Mechanism of cotranslational protein N-myristoylation in human cells. 著者: Martin Gamerdinger / Blanca Echeverria / Alfred M Lentzsch / Nicolas Burg / Ziyi Fan / Mateusz Jaskolowski / Alain Scaiola / Selina Piening / Shu-Ou Shan / Nenad Ban / Elke Deuerling / ![]() ![]() ![]() 要旨: N-myristoyltransferases (NMTs) cotranslationally transfer the fatty acid myristic acid to the N terminus of newly synthesized proteins, regulating their function and cellular localization. These ...N-myristoyltransferases (NMTs) cotranslationally transfer the fatty acid myristic acid to the N terminus of newly synthesized proteins, regulating their function and cellular localization. These enzymes are important drug targets for the treatment of cancer and viral infections. N-myristoylation of nascent proteins occurs specifically on N-terminal glycine residues after the excision of the initiator methionine by methionine aminopeptidases (METAPs). How NMTs interact with ribosomes and gain timely and specific access to their substrates remains unknown. Here, we show that human NMT1 exchanges with METAP1 at the ribosomal tunnel exit to form an active cotranslational complex together with the nascent polypeptide-associated complex (NAC). NMT1 binding is sequence selective and specifically triggered by methionine excision, which exposes the N-myristoylation motif in the nascent chain. The revealed mode of interaction of NMT1 with NAC and the methionine-cleaved nascent protein elucidates how a specific subset of proteins can be efficiently N-myristoylated in human cells. | ||||||||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 6.6 MB | 表示 | ![]() |
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PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
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-検証レポート
文書・要旨 | ![]() | 2.7 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 3 MB | 表示 | |
XML形式データ | ![]() | 454.1 KB | 表示 | |
CIF形式データ | ![]() | 750.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 53296MC ![]() 9qqaC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
+RNA鎖 , 5種, 5分子 B5B7B8A2AT
+Large ribosomal subunit protein ... , 10種, 10分子 BABCBDBLBOBPBSBXBoBs
+Ribosomal protein ... , 10種, 10分子 BBBFBNBRBVBWBYBeBjBv
+60S ribosomal protein ... , 21種, 21分子 BEBGBHBIBJBMBTBUBZBaBbBcBdBfBgBhBiBkBlBpBt
+タンパク質・ペプチド , 1種, 1分子 BK
+タンパク質 , 8種, 8分子 BQBrNtNuMAADAEAj
+Ubiquitin-ribosomal protein ... , 2種, 2分子 BmAC
+N-alpha-acetyltransferase ... , 2種, 2分子 XAXB
+Small ribosomal subunit protein ... , 20種, 20分子 AAABAFAGAZAeAiAkAlAmAnAoApAqArAtAuAvAyAz
+40S ribosomal protein ... , 10種, 10分子 AaAbAcAdAfAgAhAsAwAx
+非ポリマー , 7種, 2696分子 












+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Quaternary complex of translating ribosome, NAC, NMT1 and NatA タイプ: RIBOSOME / Entity ID: #1-#13, #15-#89 / 由来: NATURAL |
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由来(天然) | 生物種: ![]() ![]() |
緩衝液 | pH: 7.6 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE-PROPANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: TFS KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2700 nm / 最小 デフォーカス(公称値): 700 nm |
撮影 | 電子線照射量: 60 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
EMソフトウェア | 名称: PHENIX / バージョン: 1.21.2_5419 / カテゴリ: モデル精密化 |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3次元再構成 | 解像度: 3.43 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 15709 / 対称性のタイプ: POINT |
精密化 | 交差検証法: NONE |