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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 8zhc | |||||||||||||||||||||
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タイトル | pre-frameshift complex of yeast 80S ribosome with eRF1 and mRNA of WNV | |||||||||||||||||||||
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![]() | RIBOSOME / 80S ribosome | |||||||||||||||||||||
機能・相同性 | ![]() RNA 5'-cap (guanine-N7)-methylation / RNA stabilization / translation termination factor activity / DNA/DNA annealing activity / translation release factor complex / cytoplasmic translational termination / RNA strand annealing activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of JAK1 activity / regulation of translational termination / RNA folding chaperone ...RNA 5'-cap (guanine-N7)-methylation / RNA stabilization / translation termination factor activity / DNA/DNA annealing activity / translation release factor complex / cytoplasmic translational termination / RNA strand annealing activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of JAK1 activity / regulation of translational termination / RNA folding chaperone / symbiont-mediated suppression of host apoptosis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / translation release factor activity, codon specific / protein methylation / translation release factor activity / Negative regulators of DDX58/IFIH1 signaling / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / sequence-specific mRNA binding / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / peptidyl-tRNA hydrolase activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / regulation of polysaccharide biosynthetic process / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / nonfunctional rRNA decay / transporter complex / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / lipopolysaccharide transport / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of translational frameshifting / Protein hydroxylation / Formation of a pool of free 40S subunits / positive regulation of protein kinase activity / preribosome, large subunit precursor / flavivirin / L13a-mediated translational silencing of Ceruloplasmin expression / Gram-negative-bacterium-type cell outer membrane assembly / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational elongation / Eukaryotic Translation Termination / ribosomal large subunit export from nucleus / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / G-protein alpha-subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / 90S preribosome / Ub-specific processing proteases / ribosomal subunit export from nucleus / regulation of translational fidelity / positive regulation of viral genome replication / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translational termination / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / translation regulator activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / cytosolic ribosome / rescue of stalled ribosome / cellular response to amino acid starvation / protein kinase C binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / macroautophagy / translational initiation / small-subunit processome / modification-dependent protein catabolic process / protein tag activity / Regulation of expression of SLITs and ROBOs / protein-DNA complex / maintenance of translational fidelity / cell outer membrane / cytoplasmic stress granule / rRNA processing / viral capsid / ribosome biogenesis / peptidase activity / double-stranded RNA binding / nucleoside-triphosphate phosphatase 類似検索 - 分子機能 | |||||||||||||||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.3 Å | |||||||||||||||||||||
![]() | Wu, M. / Yuan, S. | |||||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Flexibilities and conservations in the structural basis of viral -1 programmed ribosomal frameshifting 著者: Wu, M. / Wan, X. / Zhou, H. / Yuan, S. | |||||||||||||||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 4.9 MB | 表示 | ![]() |
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PDB形式 | ![]() | 表示 | ![]() | |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 2.1 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 2.6 MB | 表示 | |
XML形式データ | ![]() | 394.5 KB | 表示 | |
CIF形式データ | ![]() | 652.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 60098MC ![]() 8zgrC ![]() 8zgyC ![]() 8zh3C ![]() 8zhbC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
-RNA鎖 , 6種, 6分子 LALBLCS2TamR
#1: RNA鎖 | 分子量: 1096497.250 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() ![]() 参照: GenBank: 1262303 |
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#2: RNA鎖 | 分子量: 38951.105 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() ![]() 参照: GenBank: 834774822 |
#3: RNA鎖 | 分子量: 50682.922 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() ![]() 参照: GenBank: 1531397035 |
#45: RNA鎖 | 分子量: 579432.750 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() ![]() 参照: GenBank: 831416138 |
#79: RNA鎖 | 分子量: 24880.865 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() ![]() |
#81: RNA鎖 | 分子量: 7975.765 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
+Large ribosomal subunit protein ... , 41種, 41分子 LDLELFLGLHLILJLKLLLMLNLOLPLQLRLSLTLULVLWLXLYLZLaLbLcLdLeLfLg...
+Small ribosomal subunit protein ... , 33種, 33分子 SASBSCSDSESFSGSHSISJSKSLSMSNSOSPSQSRSSSTSUSVSWSXSYSZSaSbScSd...
-タンパク質 / タンパク質・ペプチド , 2種, 2分子 eRpp
#80: タンパク質 | 分子量: 50224.996 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: P62495 |
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#82: タンパク質・ペプチド | 分子量: 3813.258 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) ![]() 発現宿主: ![]() ![]() |
-詳細
Has protein modification | N |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: 80S ribosome / タイプ: RIBOSOME / Entity ID: all / 由来: NATURAL |
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由来(天然) | 生物種: ![]() ![]() |
緩衝液 | pH: 7.5 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
顕微鏡 | モデル: JEOL CRYO ARM 300 |
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電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 1000 nm |
撮影 | 電子線照射量: 50 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3次元再構成 | 解像度: 2.3 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 460403 / 対称性のタイプ: POINT |