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- PDB-8z5x: Cryo-EM structure of the MS ring with the proximal rod and the ex... -

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Basic information

Entry
Database: PDB / ID: 8z5x
TitleCryo-EM structure of the MS ring with the proximal rod and the export apparatus (C1) within the polar flagellar motor
Components
  • (Flagellar biosynthetic protein ...) x 4
  • Alanine-modelled FlgB-Dc loop
  • Flagellar M-ring protein
  • Flagellar basal body rod protein FlgB
  • Flagellar basal-body rod protein FlgC
  • Flagellar hook-basal body complex protein FliE
  • FliE-helix 1
KeywordsMOTOR PROTEIN / Polar flagellum / Polar flagellar motor / MS ring
Function / homology
Function and homology information


bacterial-type flagellum basal body, rod / bacterial-type flagellum organization / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / cytoskeletal motor activity / bacterial-type flagellum assembly / protein secretion / bacterial-type flagellum-dependent cell motility / protein targeting ...bacterial-type flagellum basal body, rod / bacterial-type flagellum organization / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / cytoskeletal motor activity / bacterial-type flagellum assembly / protein secretion / bacterial-type flagellum-dependent cell motility / protein targeting / structural molecule activity / plasma membrane
Similarity search - Function
Flagellar hook-basal body complex protein FliE / Flagellar hook-basal body complex protein FliE / Flagellar basal-body rod protein FlgC / Flagellar biosynthesis protein FliQ / Flagellar basal-body rod protein FlgB / Flagellar transport protein FliP / Flagellar biosynthesis protein FliR / Bacterial export protein family 3 / Bacterial export proteins, family 3 / Flagella transport protein fliP family signature 1. ...Flagellar hook-basal body complex protein FliE / Flagellar hook-basal body complex protein FliE / Flagellar basal-body rod protein FlgC / Flagellar biosynthesis protein FliQ / Flagellar basal-body rod protein FlgB / Flagellar transport protein FliP / Flagellar biosynthesis protein FliR / Bacterial export protein family 3 / Bacterial export proteins, family 3 / Flagella transport protein fliP family signature 1. / Type III secretion system inner membrane P protein / FliP family / Flagella transport protein fliP family signature 2. / Type III secretion system inner membrane R protein / Bacterial export proteins, family 1 / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal / Flagellar basal-body/hook protein, C-terminal domain / Flagella basal body rod protein / Flagellar basal body rod FlgEFG protein C-terminal / Flagellar M-ring protein FliF / Flagellar M-ring C-terminal / Flagellar M-ring protein C-terminal / Lipoprotein YscJ/Flagellar M-ring protein / Secretory protein of YscJ/FliF family / Flagellar M-ring , N-terminal / AMP-binding enzyme, C-terminal domain superfamily
Similarity search - Domain/homology
Flagellar basal body rod protein FlgB / Flagellar basal-body rod protein FlgC / Flagellar biosynthetic protein FliR / Flagellar biosynthetic protein FliQ / Flagellar biosynthetic protein FliP / Flagellar hook-basal body complex protein FliE / : / Flagellar M-ring protein
Similarity search - Component
Biological speciesVibrio alginolyticus (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.64 Å
AuthorsZhang, L. / Tan, J.X. / Zhou, Y. / Zhu, Y.Q.
Funding support China, 3items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81925024 China
National Natural Science Foundation of China (NSFC)U23A20163 China
Ministry of Science and Technology (MoST, China)2017YFA0503900 China
CitationJournal: To Be Published
Title: Cryo-EM structure of the MS ring with the proximal rod and the export apparatus (C1) within the polar flagellar motor
Authors: Zhang, L. / Tan, J.X. / Zhou, Y. / Zhu, Y.Q.
History
DepositionApr 18, 2024Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Oct 22, 2025Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
0: Flagellar biosynthetic protein FliP
1: Flagellar M-ring protein
2: Flagellar M-ring protein
3: Flagellar M-ring protein
4: Flagellar M-ring protein
5: Flagellar M-ring protein
6: Flagellar biosynthetic protein FliQ
7: Flagellar biosynthetic protein FliQ
8: Flagellar biosynthetic protein FliR
9: Flagellar biosynthetic protein FliP
A: Flagellar M-ring protein
AA: Flagellar M-ring protein
AB: Flagellar M-ring protein
AC: Flagellar M-ring protein
AD: Flagellar M-ring protein
AE: Flagellar M-ring protein
AF: Flagellar M-ring protein
AG: Flagellar M-ring protein
AH: Flagellar M-ring protein
AI: Flagellar M-ring protein
AJ: Flagellar M-ring protein
AK: Flagellar M-ring protein
AL: Flagellar M-ring protein
AM: Flagellar M-ring protein
AN: Flagellar M-ring protein
AO: Flagellar M-ring protein
AP: Flagellar biosynthetic protein FliP
AQ: Flagellar biosynthetic protein FliQ
AR: Flagellar biosynthetic protein FliQ
AS: Flagellar basal body rod protein FlgB
AT: Flagellar basal body rod protein FlgB
AU: Flagellar basal body rod protein FlgB
AV: Flagellar basal body rod protein FlgB
AW: Flagellar basal body rod protein FlgB
AX: Flagellar basal-body rod protein FlgC
AY: Flagellar basal-body rod protein FlgC
AZ: Flagellar basal-body rod protein FlgC
Aa: Flagellar basal-body rod protein FlgC
Ab: Flagellar basal-body rod protein FlgC
Ac: Flagellar basal-body rod protein FlgC
Ad: Flagellar hook-basal body complex protein FliE
Ae: Flagellar hook-basal body complex protein FliE
Af: Flagellar hook-basal body complex protein FliE
Ag: Flagellar hook-basal body complex protein FliE
Ah: Flagellar hook-basal body complex protein FliE
Ai: Flagellar hook-basal body complex protein FliE
Aj: Flagellar biosynthetic protein FlhB
Ak: Flagellar biosynthetic protein FliP
Al: Flagellar biosynthetic protein FliP
B: Flagellar M-ring protein
C: Flagellar M-ring protein
D: Flagellar M-ring protein
E: Flagellar M-ring protein
F: Flagellar M-ring protein
G: Flagellar M-ring protein
H: Flagellar M-ring protein
I: Flagellar M-ring protein
J: Flagellar M-ring protein
K: Flagellar M-ring protein
L: Flagellar M-ring protein
M: Flagellar M-ring protein
N: Flagellar M-ring protein
O: Flagellar M-ring protein
P: Flagellar M-ring protein
Q: Flagellar M-ring protein
R: Flagellar M-ring protein
S: Flagellar M-ring protein
T: Flagellar M-ring protein
U: Flagellar M-ring protein
V: Flagellar M-ring protein
W: Flagellar M-ring protein
X: Flagellar M-ring protein
Y: Flagellar M-ring protein
Z: Flagellar M-ring protein
a: Flagellar M-ring protein
b: Flagellar M-ring protein
c: Flagellar M-ring protein
d: Flagellar M-ring protein
e: Flagellar M-ring protein
f: Flagellar M-ring protein
g: Flagellar M-ring protein
h: Flagellar M-ring protein
i: Flagellar M-ring protein
j: Flagellar M-ring protein
k: Flagellar M-ring protein
l: Flagellar M-ring protein
m: Flagellar M-ring protein
n: Flagellar M-ring protein
o: Flagellar M-ring protein
p: Flagellar M-ring protein
q: Flagellar M-ring protein
r: Flagellar M-ring protein
s: Flagellar M-ring protein
t: Flagellar M-ring protein
u: Flagellar M-ring protein
v: Flagellar M-ring protein
w: Flagellar M-ring protein
x: Flagellar M-ring protein
y: Flagellar M-ring protein
z: Flagellar M-ring protein
Am: Alanine-modelled FlgB-Dc loop
An: Alanine-modelled FlgB-Dc loop
Ao: Alanine-modelled FlgB-Dc loop
Ap: Alanine-modelled FlgB-Dc loop
Aq: Alanine-modelled FlgB-Dc loop
Ar: FliE-helix 1
As: FliE-helix 1
At: FliE-helix 1
Au: FliE-helix 1
Av: FliE-helix 1
Aw: FliE-helix 1


Theoretical massNumber of molelcules
Total (without water)5,115,989111
Polymers5,115,989111
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Flagellar biosynthetic protein ... , 4 types, 11 molecules 09APAkAl67AQAR8Aj

#1: Protein
Flagellar biosynthetic protein FliP


Mass: 31314.904 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A1W6TTE6
#3: Protein
Flagellar biosynthetic protein FliQ


Mass: 10329.615 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0T7EAU9
#4: Protein Flagellar biosynthetic protein FliR


Mass: 28905.535 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0YFV7
#8: Protein Flagellar biosynthetic protein FlhB


Mass: 42197.297 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0AAE4M6S6

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Protein , 4 types, 89 molecules 12345AAAABACADAEAFAGAHAIAJAKALAMANAOBCDEFGHIJ...

#2: Protein ...
Flagellar M-ring protein


Mass: 63915.605 Da / Num. of mol.: 72 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: Q75N27
#5: Protein
Flagellar basal body rod protein FlgB


Mass: 14239.950 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0G9LZG6
#6: Protein
Flagellar basal-body rod protein FlgC


Mass: 14812.569 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0G9LZH2
#7: Protein
Flagellar hook-basal body complex protein FliE


Mass: 11135.679 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A2L2K9J6

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Protein/peptide , 2 types, 11 molecules AmAnAoApAqArAsAtAuAvAw

#9: Protein/peptide
Alanine-modelled FlgB-Dc loop


Mass: 1395.589 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148
#10: Protein/peptide
FliE-helix 1


Mass: 1867.152 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148

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Details

Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: The polar flagellar motor-hook complex / Type: COMPLEX / Entity ID: all / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Vibrio alginolyticus (bacteria) / Strain: KK148
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 105000 X / Nominal defocus max: 1800 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)
EM imaging opticsEnergyfilter name: TFS Selectris / Energyfilter slit width: 10 eV

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Processing

EM software
IDNameVersionCategory
1cryoSPARCparticle selection
2EPUimage acquisition
4cryoSPARCCTF correction
7Cootmodel fitting
8UCSF ChimeraXmodel fitting
10cryoSPARCinitial Euler assignment
11cryoSPARCfinal Euler assignment
13cryoSPARC3D reconstruction
14PHENIX1.20.1_4487:model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 329955
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 3.64 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 58418 / Symmetry type: POINT
Atomic model buildingProtocol: FLEXIBLE FIT / Space: REAL
Atomic model building
ID 3D fitting-IDSource nameTypeDetails
11AlphaFoldin silico model
21Otherin silico modelModelAngelo

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