[English] 日本語
![](img/lk-miru.gif)
- PDB-8uh4: Cryo-EM structure of Maize Streak Virus (MSV) - single head Gemin... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8uh4 | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of Maize Streak Virus (MSV) - single head Geminivirus | ||||||
![]() |
| ||||||
![]() | VIRUS/DNA / geminivirus / single-head / maize / icosahedron / VIRUS-DNA complex | ||||||
Function / homology | ![]() T=1 icosahedral viral capsid / viral penetration into host nucleus / host cell / symbiont entry into host cell / host cell nucleus / structural molecule activity / DNA binding Similarity search - Function | ||||||
Biological species | Maize streak virus genotype A | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.72 Å | ||||||
![]() | Bennett, A.B. / McKenna, R. / Mietzsch, M. / Hull, J.A. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: The Two States of MSV Gemini Capsid Assembly Authors: Bennett, A.D. / McKenna, R. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 2.3 MB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 268.7 KB | Display | |
Data in CIF | ![]() | 377.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 42246MC M: map data used to model this data C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 26880.529 Da / Num. of mol.: 60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: isolate Nigeria / Gene: V1 / Production host: ![]() #2: Protein/peptide | Mass: 2755.787 Da / Num. of mol.: 60 / Source method: obtained synthetically Source: (synth.) ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-
Sample preparation
Component | Name: Maize streak virus - [Nigeria] / Type: VIRUS / Entity ID: #1 / Source: RECOMBINANT |
---|---|
Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Details of virus | Empty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION |
Natural host | Organism: Zea mays |
Buffer solution | pH: 4 |
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: DIRECT ELECTRON DE-64 (8k x 8k) |
-
Processing
EM software | Name: Leginon / Category: image acquisition | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.72 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 43111 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|