+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8ssa | |||||||||||||||
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タイトル | Structure of AMPA receptor GluA2 complex with auxiliary subunits TARP gamma-5 and cornichon-2 bound to glutamate and channel blocker spermidine (desensitized state) | |||||||||||||||
要素 |
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キーワード | SIGNALING PROTEIN / AMPA receptor / spermidine / TARP gamma-5 / cornichon-2 | |||||||||||||||
機能・相同性 | 機能・相同性情報 neurotransmitter receptor transport, postsynaptic endosome to lysosome / regulation of AMPA receptor activity / Cargo concentration in the ER / neurotransmitter receptor internalization / postsynaptic neurotransmitter receptor diffusion trapping / COPII-mediated vesicle transport / spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors ...neurotransmitter receptor transport, postsynaptic endosome to lysosome / regulation of AMPA receptor activity / Cargo concentration in the ER / neurotransmitter receptor internalization / postsynaptic neurotransmitter receptor diffusion trapping / COPII-mediated vesicle transport / spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / transmission of nerve impulse / ligand-gated monoatomic cation channel activity / channel regulator activity / response to lithium ion / Trafficking of GluR2-containing AMPA receptors / extracellularly glutamate-gated ion channel activity / immunoglobulin binding / AMPA glutamate receptor complex / kainate selective glutamate receptor activity / ionotropic glutamate receptor complex / cellular response to glycine / asymmetric synapse / regulation of receptor recycling / Unblocking of NMDA receptors, glutamate binding and activation / voltage-gated calcium channel activity / glutamate receptor binding / positive regulation of synaptic transmission / extracellular ligand-gated monoatomic ion channel activity / glutamate-gated receptor activity / response to fungicide / glutamate-gated calcium ion channel activity / presynaptic active zone membrane / vesicle-mediated transport / regulation of synaptic transmission, glutamatergic / ionotropic glutamate receptor binding / somatodendritic compartment / dendrite membrane / cellular response to brain-derived neurotrophic factor stimulus / endoplasmic reticulum-Golgi intermediate compartment membrane / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / cytoskeletal protein binding / dendrite cytoplasm / ionotropic glutamate receptor signaling pathway / positive regulation of synaptic transmission, glutamatergic / SNARE binding / dendritic shaft / synaptic membrane / synaptic transmission, glutamatergic / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / PDZ domain binding / protein tetramerization / postsynaptic density membrane / establishment of protein localization / modulation of chemical synaptic transmission / ER to Golgi transport vesicle membrane / Schaffer collateral - CA1 synapse / terminal bouton / receptor internalization / cerebral cortex development / synaptic vesicle membrane / synaptic vesicle / presynapse / signaling receptor activity / presynaptic membrane / amyloid-beta binding / growth cone / scaffold protein binding / chemical synaptic transmission / perikaryon / postsynaptic membrane / dendritic spine / postsynaptic density / neuron projection / axon / neuronal cell body / glutamatergic synapse / dendrite / synapse / endoplasmic reticulum membrane / protein-containing complex binding / protein kinase binding / cell surface / endoplasmic reticulum / protein-containing complex / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 | |||||||||||||||
生物種 | Rattus norvegicus (ドブネズミ) Homo sapiens (ヒト) | |||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.88 Å | |||||||||||||||
データ登録者 | Gangwar, S.P. / Yen, L.Y. / Yelshanskaya, M.V. / Sobolevsky, A.I. | |||||||||||||||
資金援助 | 米国, 4件
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引用 | ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Modulation of GluA2-γ5 synaptic complex desensitization, polyamine block and antiepileptic perampanel inhibition by auxiliary subunit cornichon-2. 著者: Shanti Pal Gangwar / Laura Y Yen / Maria V Yelshanskaya / Aryeh Korman / Drew R Jones / Alexander I Sobolevsky / 要旨: Synaptic complexes of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors (AMPARs) with auxiliary subunits mediate most excitatory neurotransmission and can be targeted to treat ...Synaptic complexes of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors (AMPARs) with auxiliary subunits mediate most excitatory neurotransmission and can be targeted to treat neuropsychiatric and neurological disorders, including epilepsy. Here we present cryogenic-electron microscopy structures of rat GluA2 AMPAR complexes with inhibitory mouse γ5 and potentiating human cornichon-2 (CNIH2) auxiliary subunits. CNIH2 appears to destabilize the desensitized state of the complex by reducing the separation of the upper lobes in ligand-binding domain dimers. At the same time, CNIH2 stabilizes binding of polyamine spermidine to the selectivity filter of the closed ion channel. Nevertheless, CNIH2, and to a lesser extent γ5, attenuate polyamine block of the open channel and reduce the potency of the antiepileptic drug perampanel that inhibits the synaptic complex allosterically by binding to sites in the ion channel extracellular collar. These findings illustrate the fine-tuning of synaptic complex structure and function in an auxiliary subunit-dependent manner, which is critical for the study of brain region-specific neurotransmission and design of therapeutics for disease treatment. | |||||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8ssa.cif.gz | 688.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8ssa.ent.gz | 560.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8ssa.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8ssa_validation.pdf.gz | 2.2 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8ssa_full_validation.pdf.gz | 2.3 MB | 表示 | |
XML形式データ | 8ssa_validation.xml.gz | 126.7 KB | 表示 | |
CIF形式データ | 8ssa_validation.cif.gz | 182.9 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ss/8ssa ftp://data.pdbj.org/pub/pdb/validation_reports/ss/8ssa | HTTPS FTP |
-関連構造データ
関連構造データ | 40749MC 8bhfC 8ss2C 8ss3C 8ss4C 8ss5C 8ss6C 8ss7C 8ss8C 8ss9C 8ssbC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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-要素
#1: タンパク質 | 分子量: 114978.859 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 遺伝子: Gria2, Glur2, Cacng5 / プラスミド: pEG BacMam / 細胞株 (発現宿主): HEK293S-GnTi / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P19491, UniProt: Q8VHW8 #2: タンパク質 | 分子量: 18948.420 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 細胞株: HEK293S-GnTi / 遺伝子: CNIH2, CNIL / 細胞株 (発現宿主): HEK293S-GnTi / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: Q6PI25 #3: 化合物 | ChemComp-PCW / #4: 化合物 | ChemComp-GLU / #5: 化合物 | ChemComp-SPD / | 研究の焦点であるリガンドがあるか | Y | |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: GluA2-TARP gamma5-cornichon-2 / タイプ: COMPLEX 詳細: Structure of AMPA receptor GluA2 complex with auxiliary subunits TARP gamma-5 and cornichon-2 bound to competitive antagonist ZK and channel blocker spermidine (closed state) Entity ID: #1-#2 / 由来: MULTIPLE SOURCES | |||||||||||||||||||||||||
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由来(天然) |
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由来(組換発現) |
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緩衝液 | pH: 8 | |||||||||||||||||||||||||
緩衝液成分 |
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試料 | 濃度: 4.5 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES / 詳細: Protein extracted and purified in detergent micelle | |||||||||||||||||||||||||
試料支持 | グリッドの材料: GOLD / グリッドのサイズ: 300 divisions/in. / グリッドのタイプ: UltrAuFoil | |||||||||||||||||||||||||
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277 K / 詳細: vitrification carried out in nitrogen atmosphere |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2000 nm / 最小 デフォーカス(公称値): 1000 nm / Cs: 2.7 mm / C2レンズ絞り径: 100 µm |
試料ホルダ | 凍結剤: NITROGEN 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
撮影 | 電子線照射量: 58 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
-解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
対称性 | 点対称性: C2 (2回回転対称) | ||||||||||||||||||||||||
3次元再構成 | 解像度: 3.88 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 58186 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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