+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8q7z | ||||||
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タイトル | Structure of the G. gallus 80S non-rotated ribosome | ||||||
要素 |
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キーワード | RIBOSOME / translation | ||||||
機能・相同性 | 機能・相同性情報 TNFR1-mediated ceramide production / Major pathway of rRNA processing in the nucleolus and cytosol / Protein hydroxylation / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Activation of NF-kappaB in B cells / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Spry regulation of FGF signaling / Downregulation of ERBB2:ERBB3 signaling ...TNFR1-mediated ceramide production / Major pathway of rRNA processing in the nucleolus and cytosol / Protein hydroxylation / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Activation of NF-kappaB in B cells / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Spry regulation of FGF signaling / Downregulation of ERBB2:ERBB3 signaling / APC/C:Cdc20 mediated degradation of Cyclin B / Autodegradation of Cdh1 by Cdh1:APC/C / SCF-beta-TrCP mediated degradation of Emi1 / APC/C:Cdc20 mediated degradation of Securin / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / APC-Cdc20 mediated degradation of Nek2A / EGFR downregulation / SCF(Skp2)-mediated degradation of p27/p21 / Degradation of beta-catenin by the destruction complex / TCF dependent signaling in response to WNT / Downstream TCR signaling / NRIF signals cell death from the nucleus / p75NTR recruits signalling complexes / NF-kB is activated and signals survival / Activated NOTCH1 Transmits Signal to the Nucleus / Downregulation of SMAD2/3:SMAD4 transcriptional activity / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / Senescence-Associated Secretory Phenotype (SASP) / FCERI mediated NF-kB activation / Regulation of innate immune responses to cytosolic DNA / Autodegradation of the E3 ubiquitin ligase COP1 / RAD18 and ubiquitinated PCNA-mediated recruitment of translesion polymerases / Nucleotide Excision Repair / Deactivation of the beta-catenin transactivating complex / TRAF6 mediated induction of proinflammatory cytokines / TAK1 activates NFkB by phosphorylation and activation of IKKs complex / NFkB activation mediated by RIP1 complexed with activated TLR3 / Activated TAK1 mediates p38 MAP kinase phosphorylation / Activated TAK1 mediates Jun kinases (JNK) phosphorylation and activation / activated TAK1 mediates p38 MAPK activation / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / AUF1 (hnRNP D0) binds and destabilizes mRNA / Degradation of AXIN / Degradation of DVL / Regulation of FZD by ubiquitination / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Regulation of TNFR1 signaling / TNFR1-induced NF-kappa-B signaling pathway / Hedgehog ligand biogenesis / CLEC7A (Dectin-1) signaling / Degradation of GLI1 by the proteasome / GLI3 is processed to GLI3R by the proteasome / Hedgehog 'on' state / Negative regulation of FGFR1 signaling / Negative regulation of FGFR2 signaling / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / Translesion synthesis by POLK / Translesion synthesis by POLI / Termination of translesion DNA synthesis / TNFR2 non-canonical NF-kB pathway / Negative regulation of MAPK pathway / Regulation of necroptotic cell death / MAP3K8 (TPL2)-dependent MAPK1/3 activation / HDR through Homologous Recombination (HRR) / Josephin domain DUBs / Ovarian tumor domain proteases / Formation of Incision Complex in GG-NER / Gap-filling DNA repair synthesis and ligation in GG-NER / Dual Incision in GG-NER / NFkB and MAPK activation mediated by TRAF6 upon TLR7 or TLR21 stimulation / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Regulation of TP53 Activity through Phosphorylation / Regulation of TP53 Degradation / Regulation of TP53 Activity through Methylation / Negative regulation of MET activity / Assembly of the pre-replicative complex / CDK-mediated phosphorylation and removal of Cdc6 / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Downregulation of ERBB2 signaling / VLDLR internalisation and degradation / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / E3 ubiquitin ligases ubiquitinate target proteins / RUNX1 regulates transcription of genes involved in differentiation of HSCs / Regulation of RUNX2 expression and activity / Regulation of PTEN localization / Regulation of PTEN stability and activity / Neddylation / ER Quality Control Compartment (ERQC) / NOTCH3 Activation and Transmission of Signal to the Nucleus / Peroxisomal protein import / Endosomal Sorting Complex Required For Transport (ESCRT) / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) 類似検索 - 分子機能 | ||||||
生物種 | Gallus gallus (ニワトリ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.5 Å | ||||||
データ登録者 | Nurullina, L. / Jenner, L. / Yusupov, M. | ||||||
資金援助 | フランス, 1件
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引用 | ジャーナル: To Be Published タイトル: Structural insights into inactive ribosome complexes derived from cold-treated chick embryo cells 著者: Nurullina, L. / Terrosu, S. / Jenner, L. / Myasnikov, A. / Yusupov, M. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8q7z.cif.gz | 4.8 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8q7z.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 8q7z.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8q7z_validation.pdf.gz | 1.5 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8q7z_full_validation.pdf.gz | 1.6 MB | 表示 | |
XML形式データ | 8q7z_validation.xml.gz | 323.9 KB | 表示 | |
CIF形式データ | 8q7z_validation.cif.gz | 594.8 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/q7/8q7z ftp://data.pdbj.org/pub/pdb/validation_reports/q7/8q7z | HTTPS FTP |
-関連構造データ
関連構造データ | 18168MC 8q87C M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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-要素
-RNA鎖 , 5種, 5分子 B5B8B7A2V
#1: RNA鎖 | 分子量: 1439503.375 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: GenBank: KT445934.2 |
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#2: RNA鎖 | 分子量: 50446.855 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: GenBank: KT445934.2 |
#3: RNA鎖 | 分子量: 38322.688 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: GenBank: 63782 |
#46: RNA鎖 | 分子量: 588302.312 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: GenBank: KT445934.2 |
#79: RNA鎖 | 分子量: 24593.699 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo |
-60S ribosomal protein ... , 16種, 16分子 AzBABCBEBHBJBMBPBTBVBbBdBfBgBkBr
#4: タンパク質・ペプチド | 分子量: 3473.451 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The protein was modelled according to the O. cuniculus sequence because we corresponding G. gallus sequence has not been published. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A087WNH4 |
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#5: タンパク質 | 分子量: 28086.889 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NIX0 |
#7: タンパク質 | 分子量: 46853.301 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q5ZII1 |
#9: タンパク質 | 分子量: 34014.535 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q8UWG7 |
#12: タンパク質 | 分子量: 21871.422 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A1D5NVI1 |
#14: タンパク質 | 分子量: 20269.396 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A1D5P3B1 |
#16: タンパク質 | 分子量: 15514.575 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: Q98TF9 |
#19: タンパク質 | 分子量: 21444.221 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: A0A1D5PR40 |
#23: タンパク質 | 分子量: 18622.045 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: R4GIQ2 |
#25: タンパク質 | 分子量: 14892.505 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the E1BY89 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZNU5 sequence.,We modelled the ...詳細: We modelled the protein according to the E1BY89 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZNU5 sequence.,We modelled the protein according to the E1BY89 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZNU5 sequence.,We modelled the protein according to the E1BY89 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZNU5 sequence.,We modelled the protein according to the E1BY89 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZNU5 sequence. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0ZNU5 |
#31: タンパク質 | 分子量: 8433.918 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A1L1RNZ2 |
#33: タンパク質 | 分子量: 19836.283 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A1L1RT94 |
#35: タンパク質 | 分子量: 12609.809 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the F1NQ35 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0YS49 sequence. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0YS49 |
#36: タンパク質 | 分子量: 13340.059 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A1L1S0X5 |
#40: タンパク質 | 分子量: 8238.948 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: E1BU66 |
#45: タンパク質 | 分子量: 15920.725 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q5F346 |
-Ribosomal protein ... , 19種, 19分子 BBBIBNBOBQBRBSBWBXBYBaBeBjBoADAnApAeAj
#6: タンパク質 | 分子量: 46201.055 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q5ZJZ2 |
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#13: タンパク質 | 分子量: 24506.912 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The N terminal of the protein have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N terminal of the protein ...詳細: The N terminal of the protein have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N terminal of the protein have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N terminal of the protein have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N terminal of the protein have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q08200 |
#17: タンパク質 | 分子量: 24193.258 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NQG5 |
#18: タンパク質 | 分子量: 23533.299 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The protein was modelled according to the O. cuniculus sequence from PDB entry 7O7Z (XP_002723961.2 ) because we corresponding G. gallus sequence has not been published. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo |
#20: タンパク質 | 分子量: 21411.389 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been ...詳細: The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete.,The N and C terminals have been modelled in the density according to the O. cuniculus sequence, as the G. gallus sequence in the database was incomplete. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q6EE60 |
#21: タンパク質 | 分子量: 23481.229 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: Q5ZKK8 |
#22: タンパク質 | 分子量: 20856.602 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NPD3 |
#26: タンパク質 | 分子量: 17837.100 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0XRE7 |
#27: タンパク質 | 分子量: 17701.158 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the E1BS06 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V1AGP8 and two more sequences. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo |
#28: タンパク質 | 分子量: 17303.363 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F2Z4K6 |
#30: タンパク質 | 分子量: 16851.850 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the F1NBX4 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZL98 sequence. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo |
#34: タンパク質 | 分子量: 15955.035 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: E1BVB1 |
#39: タンパク質 | 分子量: 11103.011 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: E1C810 |
#43: タンパク質 | 分子量: 12335.784 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the F1NIK4 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0YUB1 sequence. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0YUB1 |
#52: タンパク質 | 分子量: 14536.841 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the A0A1D5PQV entry although it is an obsoleted entry, because no more entry in database. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo |
#60: タンパク質 | 分子量: 16302.772 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q5ZHW8 |
#67: タンパク質 | 分子量: 16445.377 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: R4GGJ0 |
#69: タンパク質 | 分子量: 22913.453 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The protein was modelled according to the O. cuniculus sequence because we corresponding G. gallus sequence has not been published. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: G1TFM5 |
#72: タンパク質 | 分子量: 18945.898 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: E1C4N0 |
-Large ribosomal subunit protein ... , 11種, 11分子 BDBFBGBLBUBZBcBhBiBlBp
#8: タンパク質 | 分子量: 34156.391 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P22451 |
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#10: タンパク質 | 分子量: 28844.486 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q5ZJ56 |
#11: タンパク質 | 分子量: 30064.742 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P32429 |
#15: タンパク質 | 分子量: 24455.928 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P41125 |
#24: タンパク質 | 分子量: 14754.892 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q98TF8 |
#29: タンパク質 | 分子量: 15835.831 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P61355 |
#32: タンパク質 | 分子量: 12835.117 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: P67883 |
#37: タンパク質 | 分子量: 14593.624 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q98TF7 |
#38: タンパク質 | 分子量: 12331.951 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q98TF6 |
#41: タンパク質 | 分子量: 6398.745 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: Q98TF5 |
#44: タンパク質 | 分子量: 10271.335 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P32046 |
-タンパク質 , 3種, 3分子 BmACAc
#42: タンパク質 | 分子量: 14769.417 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: O42388 |
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#68: タンパク質 | 分子量: 18018.066 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / 参照: UniProt: P79781 |
#78: タンパク質 | 分子量: 26760.383 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo 参照: UniProt: F1NPA9, DNA-(apurinic or apyrimidinic site) lyase |
-40S ribosomal protein ... , 18種, 18分子 AaAAABAbAEAgAhAiAkAtAuAvAwAxAGArAsAy
#47: タンパク質 | 分子量: 29923.994 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the F2Z4K7 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XXX0 sequence.,We modelled the ...詳細: We modelled the protein according to the F2Z4K7 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XXX0 sequence.,We modelled the protein according to the F2Z4K7 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XXX0 sequence.,We modelled the protein according to the F2Z4K7 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XXX0 sequence.,We modelled the protein according to the F2Z4K7 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XXX0 sequence. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0XXX0 |
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#48: タンパク質 | 分子量: 9566.283 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A3Q2TV39 |
#49: タンパク質 | 分子量: 7855.052 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A3Q2U825 |
#50: タンパク質 | 分子量: 28875.881 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0Z891 |
#53: タンパク質 | 分子量: 13063.532 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q5ZM66 |
#55: タンパク質 | 分子量: 22153.943 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NN16 |
#56: タンパク質 | 分子量: 24112.203 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NB02 |
#57: タンパク質 | 分子量: 22641.564 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The protein was modelled according to the O. cuniculus sequence because we corresponding G. gallus sequence has not been published. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: B7NZS8 |
#58: タンパク質 | 分子量: 18384.668 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q98TH5 |
#61: タンパク質 | 分子量: 13399.751 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NH93 |
#62: タンパク質 | 分子量: 9173.397 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the E1BSJ2 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0ZQ38 and A0A8V0ZC45 sequences. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0ZQ38 |
#63: タンパク質 | 分子量: 14881.555 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A3Q3A7P6 |
#64: タンパク質 | 分子量: 15844.666 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the F1NDC2 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XRS9 sequence.,We modelled the ...詳細: We modelled the protein according to the F1NDC2 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XRS9 sequence.,We modelled the protein according to the F1NDC2 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XRS9 sequence.,We modelled the protein according to the F1NDC2 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XRS9 sequence.,We modelled the protein according to the F1NDC2 entry although it is an obsoleted entry, because the sequence fits the electron density contrary to the A0A8V0XRS9 sequence. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0XRS9 |
#65: タンパク質 | 分子量: 15237.104 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: F1NF89 |
#71: タンパク質 | 分子量: 18662.643 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V0Z8V3 |
#75: タンパク質 | 分子量: 17759.777 Da / 分子数: 1 / 由来タイプ: 天然 詳細: The protein was modelled according to the O. cuniculus sequence because we corresponding G. gallus sequence has not been published. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: G1TPG3 |
#76: タンパク質 | 分子量: 16118.546 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the A0A1D5PDV6 entry although it is an obsoleted entry, because no more sequence in database. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo |
#77: タンパク質 | 分子量: 13746.198 Da / 分子数: 1 / 由来タイプ: 天然 詳細: We modelled the protein according to the F1NU56 entry although it is an obsoleted entry, because no more sequence in database. 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: A0A8V1A7V4 |
-Small ribosomal subunit protein ... , 8種, 8分子 AdAfAmAZAFAlAoAq
#51: タンパク質 | 分子量: 29652.877 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P47836 |
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#54: タンパク質 | 分子量: 28723.727 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P47838 |
#59: タンパク質 | 分子量: 17259.389 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: Q6ITC7 |
#66: タンパク質 | 分子量: 33093.180 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P50890 |
#70: タンパク質 | 分子量: 35115.652 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P63247 |
#73: タンパク質 | 分子量: 14569.013 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P84175 |
#74: タンパク質 | 分子量: 17076.207 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P62846 |
#80: タンパク質 | 分子量: 15566.146 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Gallus gallus (ニワトリ) / Plasmid details: embryo / 参照: UniProt: P08636 |
-非ポリマー , 3種, 6407分子
#81: 化合物 | ChemComp-MG / #82: 化合物 | ChemComp-ZN / #83: 水 | ChemComp-HOH / | |
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-詳細
研究の焦点であるリガンドがあるか | N |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: Gallus gallus 80S ribosome / タイプ: RIBOSOME / 詳細: Contains endogenous E-site tRNA / Entity ID: #1-#80 / 由来: NATURAL |
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分子量 | 値: 4.2 MDa / 実験値: NO |
由来(天然) | 生物種: Gallus gallus (ニワトリ) |
緩衝液 | pH: 7.5 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE / 湿度: 95 % |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD / 倍率(公称値): 96000 X / 最大 デフォーカス(公称値): 1500 nm / 最小 デフォーカス(公称値): 300 nm |
撮影 | 電子線照射量: 40 e/Å2 フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) |
-解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3次元再構成 | 解像度: 2.5 Å / 解像度の算出法: FSC 3 SIGMA CUT-OFF / 粒子像の数: 122598 / アルゴリズム: FOURIER SPACE / クラス平均像の数: 1 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
原子モデル構築 | プロトコル: RIGID BODY FIT / 空間: REAL / Target criteria: Cross-correlation coefficient | ||||||||||||||||||||||||
原子モデル構築 | PDB-ID: 7O7Z Accession code: 7O7Z / Source name: PDB / タイプ: experimental model | ||||||||||||||||||||||||
拘束条件 |
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