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Open data
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Basic information
Entry | Database: PDB / ID: 8k7t | ||||||
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Title | Mouse Fc epsilon RI in complex with mIgE Fc | ||||||
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![]() | IMMUNE SYSTEM / IgE / high-affinity IgE receptor / allergy | ||||||
Function / homology | ![]() Platelet Adhesion to exposed collagen / IgE receptor activity / Fc-epsilon receptor I complex / serotonin secretion / Fc epsilon receptor (FCERI) signaling / Dectin-2 family / Fc receptor mediated stimulatory signaling pathway / Role of LAT2/NTAL/LAB on calcium mobilization / T cell differentiation involved in immune response / negative regulation of mast cell apoptotic process ...Platelet Adhesion to exposed collagen / IgE receptor activity / Fc-epsilon receptor I complex / serotonin secretion / Fc epsilon receptor (FCERI) signaling / Dectin-2 family / Fc receptor mediated stimulatory signaling pathway / Role of LAT2/NTAL/LAB on calcium mobilization / T cell differentiation involved in immune response / negative regulation of mast cell apoptotic process / high-affinity IgE receptor activity / GPVI-mediated activation cascade / FCERI mediated MAPK activation / Fc-gamma receptor III complex / mast cell apoptotic process / mast cell activation / FCERI mediated Ca+2 mobilization / serotonin secretion by platelet / positive regulation of interleukin-3 production / FCERI mediated NF-kB activation / Cell surface interactions at the vascular wall / neutrophil activation involved in immune response / positive regulation of mast cell cytokine production / Fc-gamma receptor signaling pathway / positive regulation of mast cell degranulation / regulation of platelet activation / positive regulation of type III hypersensitivity / IgE binding / positive regulation of type IIa hypersensitivity / regulation of release of sequestered calcium ion into cytosol / positive regulation of protein localization to cell surface / : / leukotriene biosynthetic process / positive regulation of type I hypersensitivity / interleukin-3-mediated signaling pathway / positive regulation of granulocyte macrophage colony-stimulating factor production / IgG binding / mast cell degranulation / phagocytosis, engulfment / positive regulation of interleukin-4 production / antigen processing and presentation of exogenous peptide antigen via MHC class I / Fc-epsilon receptor signaling pathway / positive regulation of interleukin-10 production / cellular response to low-density lipoprotein particle stimulus / regulation of immune response / immunoglobulin mediated immune response / positive regulation of phagocytosis / positive regulation of calcium-mediated signaling / neutrophil chemotaxis / Neutrophil degranulation / SH2 domain binding / osteoclast differentiation / establishment of localization in cell / protein localization to plasma membrane / integrin-mediated signaling pathway / phosphoprotein binding / calcium-mediated signaling / receptor internalization / positive regulation of interleukin-6 production / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of immune response / positive regulation of tumor necrosis factor production / cell surface receptor signaling pathway / endosome / defense response to bacterium / immune response / protein heterodimerization activity / membrane raft / external side of plasma membrane / innate immune response / protein kinase binding / cell surface / signal transduction / protein homodimerization activity / identical protein binding / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.71 Å | ||||||
![]() | Zhang, Z. / Yui, M. / Ohto, U. / Shimizu, T. | ||||||
Funding support | 1items
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![]() | ![]() Title: Mouse Fc epsilon RI in complex withMouse Fc epsilon RI in complex with mIgE Fc mIgE Fc Authors: Zhang, Z. / Yui, M. / Ohto, U. / Shimizu, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 215.7 KB | Display | ![]() |
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PDB format | ![]() | 169.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 36941MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
-High affinity immunoglobulin epsilon receptor subunit ... , 3 types, 4 molecules AGHB
#1: Protein | Mass: 31646.197 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Protein | Mass: 11890.856 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Protein | | Mass: 27166.141 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Protein , 1 types, 2 molecules EF
#4: Protein | Mass: 40369.723 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 3 types, 11 molecules ![](data/chem/img/NAG.gif)
#5: Polysaccharide | Source method: isolated from a genetically manipulated source #6: Polysaccharide | Source method: isolated from a genetically manipulated source #7: Sugar | ChemComp-NAG / |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Mouse Fc epsilon RI in complex with mIgE Fc / Type: COMPLEX / Entity ID: #1-#4 / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: TFS KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 61 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.71 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 91911 / Symmetry type: POINT | ||||||||||||||||||||||||
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